Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme
Abstract
Cytochrome P450 CYP153A6 from Myobacterium sp. strain HXN1500 was engineered using in-vivo directed evolution to hydroxylate small-chain alkanes regioselectively. Mutant CYP153A6-BMO1 selectively hydroxylates butane and pentane at the terminal carbon to form 1-butanol and 1-pentanol, respectively, at rates greater than wild-type CYP153A6 enzymes. This biocatalyst is highly active for small-chain alkane substrates and the regioselectivity is retained in whole-cell biotransformations.
- Inventors:
- Issue Date:
- Research Org.:
- California Institute of Technology (CalTech), Pasadena, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1082682
- Patent Number(s):
- 8361769
- Application Number:
- 12/619,033
- Assignee:
- U.S. Department of Energy (Washington, DC)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG02-06ER15762
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2009 Nov 16
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Koch, Daniel J., and Arnold, Frances H. Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme. United States: N. p., 2013.
Web.
Koch, Daniel J., & Arnold, Frances H. Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme. United States.
Koch, Daniel J., and Arnold, Frances H. Tue .
"Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme". United States. https://www.osti.gov/servlets/purl/1082682.
@article{osti_1082682,
title = {Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme},
author = {Koch, Daniel J. and Arnold, Frances H.},
abstractNote = {Cytochrome P450 CYP153A6 from Myobacterium sp. strain HXN1500 was engineered using in-vivo directed evolution to hydroxylate small-chain alkanes regioselectively. Mutant CYP153A6-BMO1 selectively hydroxylates butane and pentane at the terminal carbon to form 1-butanol and 1-pentanol, respectively, at rates greater than wild-type CYP153A6 enzymes. This biocatalyst is highly active for small-chain alkane substrates and the regioselectivity is retained in whole-cell biotransformations.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2013},
month = {1}
}
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Works referenced in this record:
Engineered Alkane-Hydroxylating Cytochrome P450BM3 Exhibiting Nativelike Catalytic Properties
journal, November 2007
- Fasan, Rudi; Chen, Mike M.; Crook, Nathan C.
- Angewandte Chemie International Edition, Vol. 46, Issue 44, p. 8414-8418
Engineering cytochrome P450cam into an alkane hydroxylase
journal, January 2003
- Bell, Stephen G.; Orton, Erica; Boyd, Helen
- Dalton Transactions, Issue 11
Alkane hydroxylases involved in microbial alkane degradation
journal, January 2007
- van Beilen, Jan B.; Funhoff, Enrico G.
- Applied Microbiology and Biotechnology, Vol. 74, Issue 1
Regio- and Enantioselective Alkane Hydroxylation with Engineered Cytochromes P450 BM-3
journal, November 2003
- Peters, Matthew W.; Meinhold, Peter; Glieder, Anton
- Journal of the American Chemical Society, Vol. 125, Issue 44, p. 13442-13450
In Vivo Evolution of Butane Oxidation by Terminal Alkane Hydroxylases AlkB and CYP153A6
journal, November 2008
- Koch, D. J.; Chen, M. M.; van Beilen, J. B.
- Applied and Environmental Microbiology, Vol. 75, Issue 2