Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor
Abstract
The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.
- Inventors:
-
- Gainesville, FL
- Tamil Nadu, IN
- Issue Date:
- Research Org.:
- University of Florida Research Foundation, Inc. (Gainesville, FL)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 983106
- Patent Number(s):
- 7326551
- Application Number:
- 10/136,960
- Assignee:
- University of Florida Research Foundation, Inc. (Gainesville, FL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- DOE Contract Number:
- FG02-96ER20222
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, and Ingram, Lonnie O. Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor. United States: N. p., 2008.
Web.
Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, & Ingram, Lonnie O. Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor. United States.
Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, and Ingram, Lonnie O. Tue .
"Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor". United States. https://www.osti.gov/servlets/purl/983106.
@article{osti_983106,
title = {Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor},
author = {Maupin-Furlow, Julie A and Talarico, Lee Ann and Raj, Krishnan Chandra and Ingram, Lonnie O},
abstractNote = {The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {2}
}
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