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Title: Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor

Abstract

The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.

Inventors:
 [1];  [1];  [2];  [1]
  1. Gainesville, FL
  2. Tamil Nadu, IN
Issue Date:
Research Org.:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Sponsoring Org.:
USDOE
OSTI Identifier:
983106
Patent Number(s):
7326551
Application Number:
10/136,960
Assignee:
University of Florida Research Foundation, Inc. (Gainesville, FL)
Patent Classifications (CPCs):
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-96ER20222
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, and Ingram, Lonnie O. Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor. United States: N. p., 2008. Web.
Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, & Ingram, Lonnie O. Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor. United States.
Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, and Ingram, Lonnie O. Tue . "Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor". United States. https://www.osti.gov/servlets/purl/983106.
@article{osti_983106,
title = {Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor},
author = {Maupin-Furlow, Julie A and Talarico, Lee Ann and Raj, Krishnan Chandra and Ingram, Lonnie O},
abstractNote = {The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2008},
month = {2}
}

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Works referenced in this record:

Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability
journal, March 2001


Cloning and expression of the Zymomonas mobilis “Production of Ethanol” Genes in Lactobacillus casei
journal, October 1996


Fuel Ethanol from Cellulosic Biomass
journal, March 1991


Cloning and Characterization of the Zymobacter palmae Pyruvate Decarboxylase Gene (pdc) and Comparison to Bacterial Homologues
journal, June 2002


Pyruvate decarboxylase of Zymomonas mobilis: isolation, properties, and genetic expression in Escherichia coli.
journal, January 1987


Thermal stability and protein structure
journal, December 1979


Expression of theZymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes inBacillus
journal, May 1994


Genetic engineering of ethanol production in Escherichia coli.
journal, January 1987


Pyruvate decarboxylase: a key enzyme for the oxidative metabolism of lactic acid by Acetobacter pasteurianus
journal, December 2001


Structure-stability relationships in proteins: new approaches to stabilizing enzymes
journal, February 1984


The N-end rule in bacteria
journal, November 1991


Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase.
journal, January 1987


Enteric Bacterial Catalysts for Fuel Ethanol Production
journal, October 1999


Biochemical and genetic characterization of the acetaldehyde dehydrogenase complex from Acetobacter europaeus
journal, August 1997


Characteristics of alcohol/polyol dehydrogenases. The zinc-containing long-chain alcohol dehydrogenases
journal, September 1987


Pyruvate decarboxylase from Zymomonas mobilis. Isolation and partial characterization
journal, November 1986


The 59-kDa polypeptide constituent of 8–10-nm cytoplasmic filaments in Neurospora crassa is a pyruvate decarboxylase
journal, August 1993


Detection of specific sequences among DNA fragments separated by gel electrophoresis
journal, November 1975


Kinetic aspects of regulation of pyruvic decarboxylase
journal, January 1976


Subunit structure, function and organisation of pyruvate decarboxylases from various organisms
journal, June 1998


The Role of His113 and His114 in Pyruvate Decarboxylase from Zymomonas Mobilis
journal, August 1997


The structural basis of substrate activation in yeast pyruvate decarboxylase: A crystallographic and kinetic study
journal, February 2000


Structural and Genetic Analysis of Protein Stability
journal, June 1993


DNA sequencing with chain-terminating inhibitors
journal, December 1977


N-Terminal processing: the methionine aminopeptidase and Nα-acetyl transferase families
journal, July 1998


Ethanol Synthesis by Genetic Engineering in Cyanobacteria
journal, February 1999


A common structural motif in thiamin pyrophosphate-binding enzymes
journal, September 1989


Nucleotide sequence of the pyruvate decarboxylase gene from Zymomonas mobilis
journal, January 1987


Simultaneous expression of genes encoding endoglucanase and ?-glucosidase in Zymomonas mobilis
journal, September 1993


Structure and properties of pyruvate decarboxylase and site-directed mutagenesis of the Zymomonas mobilis enzyme
journal, June 1998


Issues in predicting protein function from sequence
journal, January 2001


Fermentation of lignocellulosic hydrolysates for ethanol production
journal, April 1996


Cloning of the Acetobacter xylinum cellulase gene and its expression in Escherichia coli and Zymomonas mobilis
journal, December 1994


Expression of a thermostable cellulase gene from a thermophilic anaerobe in Saccharomyces cerevisiae
journal, January 1990


CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994


Purification and characterization of pyruvate decarboxylase from Sarcina ventriculi
journal, April 1992


Purification and Characterisation of Pyruvate Decarboxylase from Pea Seeds (Pisum sativum cv. Miko)
journal, January 1995


Which craft is best in bioinformatics?
journal, July 2001