Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor
Abstract
The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.
- Inventors:
-
- Gainesville, FL
- Tamil Nadu, IN
- Issue Date:
- Research Org.:
- University of Florida Research Foundation, Inc. (Gainesville, FL)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 983106
- Patent Number(s):
- 7326551
- Application Number:
- 10/136,960
- Assignee:
- University of Florida Research Foundation, Inc. (Gainesville, FL)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- DOE Contract Number:
- FG02-96ER20222
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, and Ingram, Lonnie O. Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor. United States: N. p., 2008.
Web.
Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, & Ingram, Lonnie O. Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor. United States.
Maupin-Furlow, Julie A, Talarico, Lee Ann, Raj, Krishnan Chandra, and Ingram, Lonnie O. Tue .
"Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor". United States. https://www.osti.gov/servlets/purl/983106.
@article{osti_983106,
title = {Cloning and sequencing of pyruvate decarboxylase (PDC) genes from bacteria and uses therefor},
author = {Maupin-Furlow, Julie A and Talarico, Lee Ann and Raj, Krishnan Chandra and Ingram, Lonnie O},
abstractNote = {The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Feb 05 00:00:00 EST 2008},
month = {Tue Feb 05 00:00:00 EST 2008}
}
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Works referenced in this record:
Catabolic Activities of Aerobic Heterotrophs
book, January 1986
- Gottschalk, Gerhard
- Bacterial Metabolism
Hyperthermophilic Enzymes: Sources, Uses, and Molecular Mechanisms for Thermostability
journal, March 2001
- Vieille, C.; Zeikus, G. J.
- Microbiology and Molecular Biology Reviews, Vol. 65, Issue 1, p. 1-43
Cloning and expression of the Zymomonas mobilis “Production of Ethanol” Genes in Lactobacillus casei
journal, October 1996
- Gold, R. Shane; Meagher, Michael M.; Tong, Suxiang
- Current Microbiology, Vol. 33, Issue 4
Fuel Ethanol from Cellulosic Biomass
journal, March 1991
- Lynd, L. R.; Cushman, J. H.; Nichols, R. J.
- Science, Vol. 251, Issue 4999
Cloning and Characterization of the Zymobacter palmae Pyruvate Decarboxylase Gene (pdc) and Comparison to Bacterial Homologues
journal, June 2002
- Raj, Krishnan Chandra; Talarico, Lee A.; Ingram, Lonnie O.
- Applied and Environmental Microbiology, Vol. 68, Issue 6
Cloning, sequencing, and characterization of the gene encoding the smallest subunit of the three-component membrane-bound alcohol dehydrogenase from Acetobacter pasteurianus.
journal, January 1995
- Kondo, K.; Beppu, T.; Horinouchi, S.
- Journal of bacteriology, Vol. 177, Issue 17
Pyruvate Decarboxylase from Zea mays L.: I. Purification and Partial Characterization from Mature Kernels and Anaerobically Treated Roots
journal, September 1985
- Lee, Thomas C.; Langston-Unkefer, Pat J.
- Plant Physiology, Vol. 79, Issue 1
Pyruvate decarboxylase of Zymomonas mobilis: isolation, properties, and genetic expression in Escherichia coli.
journal, January 1987
- Neale, A. D.; Scopes, R. K.; Wettenhall, R. E.
- Journal of Bacteriology, Vol. 169, Issue 3
Thermal stability and protein structure
journal, December 1979
- Argos, Patrick; Rossmann, Michael G.; Grau, Ulrich M.
- Biochemistry, Vol. 18, Issue 25
Expression of theZymomonas mobilis alcohol dehydrogenase II (adhB) and pyruvate decarboxylase (pdc) genes inBacillus
journal, May 1994
- Barbosa, Maria de F. S.; Ingram, L. O.
- Current Microbiology, Vol. 28, Issue 5
Multi-enzyme complexes in thermophilic organisms: isolation and characterization of the pyruvate dehydrogenase complex from Thermus aquaticus AT62
journal, April 1992
- Maas, E.; Pospichal, H.; KOPlin, R.
- Journal of General Microbiology, Vol. 138, Issue 4
Pyruvate decarboxylase: a key enzyme for the oxidative metabolism of lactic acid by Acetobacter pasteurianus
journal, December 2001
- Chandra Raj, Krishnan; Ingram, Lonnie; Maupin-Furlow, Julie
- Archives of Microbiology, Vol. 176, Issue 6
Structure-stability relationships in proteins: new approaches to stabilizing enzymes
journal, February 1984
- Mozhaev, V. V.; Martinek, Karel
- Enzyme and Microbial Technology, Vol. 6, Issue 2
Cloning, expression and characterization of a Family 48 exocellulase, Cel48A, from Thermobifida fusca: Thermobifida fusca exocellulase Cel48A
journal, August 2000
- Irwin, Diana C.; Zhang, Sheng; Wilson, David B.
- European Journal of Biochemistry, Vol. 267, Issue 16
The N-end rule in bacteria
journal, November 1991
- Tobias, J.; Shrader, T.; Rocap, G.
- Science, Vol. 254, Issue 5036
Promoter and nucleotide sequences of the Zymomonas mobilis pyruvate decarboxylase.
journal, January 1987
- Conway, T.; Osman, Y. A.; Konnan, J. I.
- Journal of Bacteriology, Vol. 169, Issue 3
Enteric Bacterial Catalysts for Fuel Ethanol Production
journal, October 1999
- Ingram, L. O.; Aldrich, H. C.; Borges, A. C. C.
- Biotechnology Progress, Vol. 15, Issue 5
Biochemical and genetic characterization of the acetaldehyde dehydrogenase complex from Acetobacter europaeus
journal, August 1997
- Thurner, Claudia; Vela, Cinzia; Thöny-Meyer, Linda
- Archives of Microbiology, Vol. 168, Issue 2
Characteristics of alcohol/polyol dehydrogenases. The zinc-containing long-chain alcohol dehydrogenases
journal, September 1987
- Jornvall, Hans; Persson, Bengt; Jeffery, Jonathan
- European Journal of Biochemistry, Vol. 167, Issue 2
Exploring the Cellulose/Xylan Specificity of the β-1,4-Glycanase Cex from Cellulomonas fimi through Crystallography and Mutation † , ‡
journal, April 1998
- Notenboom, Valerie; Birsan, Camelia; Warren, R. Antony J.
- Biochemistry, Vol. 37, Issue 14
Pyruvate decarboxylase from Zymomonas mobilis. Isolation and partial characterization
journal, November 1986
- Bringer-Meyer, S.; Schimz, K. -L.; Sahm, H.
- Archives of Microbiology, Vol. 146, Issue 2
The 59-kDa polypeptide constituent of 8–10-nm cytoplasmic filaments in Neurospora crassa is a pyruvate decarboxylase
journal, August 1993
- Alvarez, Maria E.; Rosa, Alberto L.; Temporini, Esteban D.
- Gene, Vol. 130, Issue 2
Detection of specific sequences among DNA fragments separated by gel electrophoresis
journal, November 1975
- Southern, E. M.
- Journal of Molecular Biology, Vol. 98, Issue 3
Kinetic aspects of regulation of pyruvic decarboxylase
journal, January 1976
- Kenworthy, P.; Davies, D. D.
- Phytochemistry, Vol. 15, Issue 2
Subunit structure, function and organisation of pyruvate decarboxylases from various organisms
journal, June 1998
- König, Stephan
- Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1385, Issue 2
The Role of His113 and His114 in Pyruvate Decarboxylase from Zymomonas Mobilis
journal, August 1997
- Schenk, Gerhard; Leeper, Finian J.; England, Renee
- European Journal of Biochemistry, Vol. 248, Issue 1
The structural basis of substrate activation in yeast pyruvate decarboxylase: A crystallographic and kinetic study
journal, February 2000
- Lu, Guoguang; Dobritzsch, Doreen; Baumann, Sabine
- European Journal of Biochemistry, Vol. 267, Issue 3
Structural and Genetic Analysis of Protein Stability
journal, June 1993
- Matthews, Brian W.
- Annual Review of Biochemistry, Vol. 62, Issue 1, p. 139-160
Production of the Gram-positive Sarcina ventriculi pyruvate decarboxylase in Escherichia coli The GenBank accession number for the sequence reported in this paper is AF354297.
journal, September 2001
- Talarico, Lee A.; Ingram, Lonnie O.; Maupin-Furlow, Julie A.
- Microbiology, Vol. 147, Issue 9
Pyruvate decarboxylase from Zymomonas mobilis. Structure and re-activation of apoenzyme by the cofactors thiamin diphosphate and magnesium ion
journal, June 1991
- Diefenbach, R. J.; Duggleby, R. G.
- Biochemical Journal, Vol. 276, Issue 2
DNA sequencing with chain-terminating inhibitors
journal, December 1977
- Sanger, F.; Nicklen, S.; Coulson, A. R.
- Proceedings of the National Academy of Sciences, Vol. 74, Issue 12
N-Terminal processing: the methionine aminopeptidase and Nα-acetyl transferase families
journal, July 1998
- Bradshaw, Ralph A.; Brickey, William W.; Walker, Kenneth W.
- Trends in Biochemical Sciences, Vol. 23, Issue 7
A common structural motif in thiamin pyrophosphate-binding enzymes
journal, September 1989
- Hawkins, Christopher F.; Borges, Adolfo; Perham, Richard N.
- FEBS Letters, Vol. 255, Issue 1
Nucleotide sequence of the pyruvate decarboxylase gene from Zymomonas mobilis
journal, January 1987
- Neale, Alan D.; Scopes, Robert K.; Wettenhall, Richard E. H.
- Nucleic Acids Research, Vol. 15, Issue 4
Simultaneous expression of genes encoding endoglucanase and ?-glucosidase in Zymomonas mobilis
journal, September 1993
- Su, P.; Liu, C. -Q; Lucas, R. J.
- Biotechnology Letters, Vol. 15, Issue 9
Structure and properties of pyruvate decarboxylase and site-directed mutagenesis of the Zymomonas mobilis enzyme
journal, June 1998
- Candy, Judith M.; Duggleby, Ronald G.
- Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, Vol. 1385, Issue 2
Issues in predicting protein function from sequence
journal, January 2001
- Ponting, C. P.
- Briefings in Bioinformatics, Vol. 2, Issue 1
Site-directed mutagenesis of the ionizable groups in the active site of Zymomonas mobilis pyruvate decarboxylase: Effect on activity and pH dependence
journal, June 2001
- Huang, Chang-Yi; Chang, Alan K.; Nixon, Peter F.
- European Journal of Biochemistry, Vol. 268, Issue 12
Fermentation of lignocellulosic hydrolysates for ethanol production
journal, April 1996
- Olsson, Lisbeth; Hahn-Hägerdal, Bärbel
- Enzyme and Microbial Technology, Vol. 18, Issue 5, p. 312-331
Cloning of the Acetobacter xylinum cellulase gene and its expression in Escherichia coli and Zymomonas mobilis
journal, December 1994
- Okamoto, T.; Yamano, S.; Ikeaga, H.
- Applied Microbiology and Biotechnology, Vol. 42, Issue 4
Expression of a thermostable cellulase gene from a thermophilic anaerobe in Saccharomyces cerevisiae
journal, January 1990
- Saito, Takao; Suzuki, Tohru; Hayashi, Akihiro
- Journal of Fermentation and Bioengineering, Vol. 69, Issue 5
Advanced Bioethanol Production Technologies: A Perspective
book, May 1997
- Himmel, Michael E.; Adney, William S.; Baker, John O.
- ACS Symposium Series
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994
- Thompson, Julie D.; Higgins, Desmond G.; Gibson, Toby J.
- Nucleic Acids Research, Vol. 22, Issue 22, p. 4673-4680
Purification and characterization of pyruvate decarboxylase from Sarcina ventriculi
journal, April 1992
- Lowe, S. E.; Zeikus, J. G.
- Journal of General Microbiology, Vol. 138, Issue 4
Purification and Characterisation of Pyruvate Decarboxylase from Pea Seeds (Pisum sativum cv. Miko)
journal, January 1995
- Mücke, Udo; König, Stephan; Hübner, Gerhard
- Biological Chemistry Hoppe-Seyler, Vol. 376, Issue 2
Comparison of the structural genes for pyruvate decarboxylase in different Zymomonas mobilis strains.
journal, January 1988
- Reynen, M.; Sahm, H.
- Journal of Bacteriology, Vol. 170, Issue 7
Bioconversion for Production of Renewable Transportation Fuels in the United States
book, October 1994
- Sheehan, John J.
- ACS Symposium Series