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Title: Species specific identification of spore-producing microbes using the gene sequence of small acid-soluble spore coat proteins for amplification based diagnostics

Patent ·
OSTI ID:874841
 [1]
  1. Decatur, GA
+ Show Author Affiliations

PCR (polymerase chain reaction) primers for the detection of certain Bacillus species, such as Bacillus anthracis. The primers specifically amplify only DNA found in the target species and can distinguish closely related species. Species-specific PCR primers for Bacillus anthracis, Bacillus globigii and Clostridium perfringens are disclosed. The primers are directed to unique sequences within sasp (small acid soluble protein) genes.

Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
DOE Contract Number:
AC03-76SF00098
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Number(s):
US 6472155
OSTI ID:
874841
Country of Publication:
United States
Language:
English

References (2)

Cloning and nucleotide sequencing of genes for a second type of small, acid-soluble spore proteins of Bacillus cereus, Bacillus stearothermophilus, and "Thermoactinomyces thalpophilus". journal January 1987
Cloning and nucleotide sequence of three genes coding for small, acid-soluble proteins of Clostridium perfringens spores journal January 1991

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Related Subjects

species
specific
identification
spore-producing
microbes
sequence
acid-soluble
spore
coat
proteins
amplification
based
diagnostics
pcr
polymerase
chain
reaction
primers
detection
bacillus
anthracis
specifically
amplify
dna
found
target
distinguish
closely
related
species-specific
globigii
clostridium
perfringens
disclosed
directed
unique
sequences
sasp
acid
soluble
protein
genes
chain reaction
/435/999/