Cloning of cellulase genes from acidothermus cellulolyticus
Abstract
A process is described for moving fragments that code for cellulase activity from the genome of A. cellulolyticus to several plasmid vectors and the subsequent expression of active cellulase acitivty in E. coli.
- Inventors:
-
- (late of Chicago, IL)
- Lakewood, CO
- Winter Haven, FL
- Issue Date:
- Research Org.:
- Midwest Research Institute, Kansas City, MO (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 870407
- Patent Number(s):
- 5514584
- Assignee:
- Midwest Research Institute (Kansas City, MO)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
- DOE Contract Number:
- AC02-83CH10093
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- cloning; cellulase; genes; acidothermus; cellulolyticus; process; described; moving; fragments; code; activity; genome; plasmid; vectors; subsequent; expression; active; acitivty; coli; acidothermus cellulolyticus; subsequent expression; active cell; cellulase activity; /435/999/
Citation Formats
Lastick, deceased, Stanley M., Tucker, Melvin P, and Grohmann, Karel. Cloning of cellulase genes from acidothermus cellulolyticus. United States: N. p., 1996.
Web.
Lastick, deceased, Stanley M., Tucker, Melvin P, & Grohmann, Karel. Cloning of cellulase genes from acidothermus cellulolyticus. United States.
Lastick, deceased, Stanley M., Tucker, Melvin P, and Grohmann, Karel. Mon .
"Cloning of cellulase genes from acidothermus cellulolyticus". United States. https://www.osti.gov/servlets/purl/870407.
@article{osti_870407,
title = {Cloning of cellulase genes from acidothermus cellulolyticus},
author = {Lastick, deceased, Stanley M. and Tucker, Melvin P and Grohmann, Karel},
abstractNote = {A process is described for moving fragments that code for cellulase activity from the genome of A. cellulolyticus to several plasmid vectors and the subsequent expression of active cellulase acitivty in E. coli.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}
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Works referenced in this record:
Cloning and expression in Escherichia coli of a Thermoanaerobacter cellulolyticus gene coding for heat-stable β-glucanase
journal, February 1987
- Honda, Hiroyuki; Naito, Hiroshi; Taya, Masahito
- Applied Microbiology and Biotechnology, Vol. 25, Issue 5