Microorganism with knock-in at acetolactate decarboxylase gene locus

Leang, Ching

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A - human necessities
A01 - agriculture
A21 - baking
A22 - butchering
A23 - foods or foodstuffs
A24 - tobacco
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B01 - physical or chemical processes or apparatus in general
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B03 - separation of solid materials using liquids or using pneumatic tables or jigs
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Title: Microorganism with knock-in at acetolactate decarboxylase gene locus

Abstract

Provided herein is a genetically engineered microorganism comprising knock-in of DNA at an acetolactate decarboxylase gene locus. Replacement of the acetolactate decarboxylase gene with DNA encoding one or more native or nonnative enzymes confers certain advantages, including fermentation stability and increased production of native and nonnative products from gaseous substrates.

Inventors:: Leang, Ching

Issue Date:: 2023-09-19

Research Org.:: LanzaTech, Inc., Skokie, IL (Untied States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 2222338

Patent Number(s):: 11760989

Application Number:: 17/303,677

Assignee:: LanzaTech, Inc. (Skokie, IL)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N9/1029 - {transferring groups other than amino-acyl groups
C12N9/13 - {transferring sulfur containing groups
C12N9/88 - Lyases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P5/007 - {containing one or more isoprene units, i.e. terpenes
C12P5/026 - {Unsaturated compounds, i.e. alkenes, alkynes or allenes}
C12P7/04 - acyclic
C12P7/065 - {with microorganisms other than yeasts}
C12P7/18 - polyhydric
C12P7/26 - Ketones
C12P7/30 - produced from substrate containing inorganic compounds other than water
C12P7/42 - Hydroxy-carboxylic acids
C12P7/6409 - {Fatty acids}

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES
C12Y208/03 - CoA-transferases
C12Y401/01001 - Pyruvate decarboxylase
C12Y401/01005 - Acetolactate decarboxylase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02E - REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
Y02E50/10 - Biofuels

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DOE Contract Number:: EE0007566

Resource Type:: Patent

Resource Relation:: Patent File Date: 06/04/2021

Country of Publication:: United States

Language:: English

Citation Formats


                    Leang, Ching. Microorganism with knock-in at acetolactate decarboxylase gene locus.  United States: N. p., 2023. 
        Web.

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                    Leang, Ching. Microorganism with knock-in at acetolactate decarboxylase gene locus.  United States.

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                    Leang, Ching. Tue .  
        "Microorganism with knock-in at acetolactate decarboxylase gene locus".  United States.  https://www.osti.gov/servlets/purl/2222338.

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@article{osti_2222338,

  title        = {Microorganism with knock-in at acetolactate decarboxylase gene locus},

  author       = {Leang, Ching},

  abstractNote = {Provided herein is a genetically engineered microorganism comprising knock-in of DNA at an acetolactate decarboxylase gene locus. Replacement of the acetolactate decarboxylase gene with DNA encoding one or more native or nonnative enzymes confers certain advantages, including fermentation stability and increased production of native and nonnative products from gaseous substrates.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2023},

  month        = {9}

}

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Works referenced in this record:

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Acetogenic production of 3-Hydroxybutyrate using a native 3-Hydroxybutyryl-CoA Dehydrogenase
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High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes
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Applied and Environmental Microbiology, Vol. 77, Issue 23, p. 8288-8294
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Fermentation process for producing isopropanol using a recombinant microorganism
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Koepke, Michael; Simpson, Sean Dennis; Liew, FungMin
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journal, July 1988

Goelling, D.; Stahl, U.
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Production and Sensing of Butyrate in a Probiotic E. coli Strain
journal, May 2020

Bai, Yanfen; Mansell, Thomas J.
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Integrated Regulation of Acetoin Fermentation by Quorum Sensing and pH in Serratia plymuthica RVH1
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Moons, Pieter; Van Houdt, Rob; Vivijs, Bram
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Similar Records

Title: Microorganism with knock-in at acetolactate decarboxylase gene locus

Abstract

Citation Formats

Novel System for Efficient Isolation of Clostridium Double-Crossover Allelic Exchange Mutants Enabling Markerless Chromosomal Gene Deletions and DNA Integration journal, September 2012

Acetogenesis and the Wood–Ljungdahl pathway of CO2 fixation journal, December 2008

Acetogenic production of 3-Hydroxybutyrate using a native 3-Hydroxybutyryl-CoA Dehydrogenase journal, August 2022

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker journal, January 2012

High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes journal, September 2011

Fermentation process for producing isopropanol using a recombinant microorganism patent, June 2016

Converting Carbon Dioxide to Butyrate with an Engineered Strain of Clostridium ljungdahlii journal, October 2014

Clostridium stain which produces acetic acid from waste gases patent, January 1997

Cloning and expression of an alpha-acetolactate decarboxylase gene from Streptococcus lactis subsp. diacetylactis in Escherichia coli journal, July 1988

Production and Sensing of Butyrate in a Probiotic E. coli Strain journal, May 2020

Integrated Regulation of Acetoin Fermentation by Quorum Sensing and pH in Serratia plymuthica RVH1 journal, May 2011

Genome editing of Clostridium autoethanogenum using CRISPR/Cas9 journal, October 2016

Clostridium ljungdahlii sp. nov., an Acetogenic Species in Clostridial rRNA Homology Group I journal, April 1993

I-SceI-mediated scarless gene modification via allelic exchange in Clostridium journal, January 2015

Two-promoter vector is highly efficient for overproduction of protein complexes journal, June 2004

Clostridium autoethanogenum, sp. nov., an anaerobic bacterium that produces ethanol from carbon monoxide journal, April 1994

Acetogenic Prokaryotes book, January 2006

ClosTron-Mediated Engineering of Clostridium book, January 2011

Microbial process for the preparation of acetic acid as well as solvent for its extraction from the fermentation broth patent, April 2002

Reconstruction of an Acetogenic 2,3-Butanediol Pathway Involving a Novel NADPH-Dependent Primary-Secondary Alcohol Dehydrogenase journal, March 2014

Fermentative production of ethanol from carbon monoxide journal, June 2011

Novel System for Efficient Isolation of Clostridium Double-Crossover Allelic Exchange Mutants Enabling Markerless Chromosomal Gene Deletions and DNA Integration
journal, September 2012

Acetogenesis and the Wood–Ljungdahl pathway of CO₂ fixation
journal, December 2008

Acetogenic production of 3-Hydroxybutyrate using a native 3-Hydroxybutyryl-CoA Dehydrogenase
journal, August 2022

Integration of DNA into bacterial chromosomes from plasmids without a counter-selection marker
journal, January 2012

High Ethanol Titers from Cellulose by Using Metabolically Engineered Thermophilic, Anaerobic Microbes
journal, September 2011

Fermentation process for producing isopropanol using a recombinant microorganism
patent, June 2016

Converting Carbon Dioxide to Butyrate with an Engineered Strain of Clostridium ljungdahlii
journal, October 2014

Clostridium stain which produces acetic acid from waste gases
patent, January 1997

Cloning and expression of an alpha-acetolactate decarboxylase gene from Streptococcus lactis subsp. diacetylactis in Escherichia coli
journal, July 1988

Production and Sensing of Butyrate in a Probiotic E. coli Strain
journal, May 2020

Integrated Regulation of Acetoin Fermentation by Quorum Sensing and pH in Serratia plymuthica RVH1
journal, May 2011

Genome editing of Clostridium autoethanogenum using CRISPR/Cas9
journal, October 2016

Clostridium ljungdahlii sp. nov., an Acetogenic Species in Clostridial rRNA Homology Group I
journal, April 1993

I-SceI-mediated scarless gene modification via allelic exchange in Clostridium
journal, January 2015

Two-promoter vector is highly efficient for overproduction of protein complexes
journal, June 2004

Clostridium autoethanogenum, sp. nov., an anaerobic bacterium that produces ethanol from carbon monoxide
journal, April 1994

Acetogenic Prokaryotes
book, January 2006

ClosTron-Mediated Engineering of Clostridium
book, January 2011

Microbial process for the preparation of acetic acid as well as solvent for its extraction from the fermentation broth
patent, April 2002

Reconstruction of an Acetogenic 2,3-Butanediol Pathway Involving a Novel NADPH-Dependent Primary-Secondary Alcohol Dehydrogenase
journal, March 2014

Fermentative production of ethanol from carbon monoxide
journal, June 2011