Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.
Abstract
A method and system for examining biological materials using low-power cw excitation Raman spectroscopy. In accordance with the teachings of the invention, a low-power continuous wave (cw) pump laser beam and a low-power cw Stokes (or anti-Stokes) probe laser beam simultaneously illuminate a biological material and traverse the biological material in collinearity. The pump beam, whose frequency is varied, is used to induce Raman emission from the biological material. The intensity of the probe beam, whose frequency is kept constant, is monitored as it leaves the biological material. When the difference between the pump and probe excitation frequencies is equal to a Raman vibrational mode frequency of the biological material, the weak probe signal becomes amplified by one or more orders of magnitude (typically up to about 10.sup.4 -10.sup.6) due to the Raman emission from the pump beam. In this manner, by monitoring the intensity of the probe beam emitted from the biological material as the pump beam is varied in frequency, one can obtain an excitation Raman spectrum for the biological material tested. The present invention may be applied to in the in vivo and/or in vitro diagnosis of diabetes, heart disease, hepatitis, cancers and other diseases by measuringmore »
- Inventors:
-
- Bronx, NY
- Flushing, NY
- Issue Date:
- Research Org.:
- City Univ. of New York (CUNY), NY (United States)
- OSTI Identifier:
- 879580
- Patent Number(s):
- 6151522
- Application Number:
- 09/270383
- Assignee:
- The Research Foundation of CUNY (New York, NY)
- Patent Classifications (CPCs):
-
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS
G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT
- DOE Contract Number:
- FG02-98ER62560
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Alfano, Robert R, and Wang, Wubao. Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.. United States: N. p., 2000.
Web.
Alfano, Robert R, & Wang, Wubao. Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.. United States.
Alfano, Robert R, and Wang, Wubao. Tue .
"Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.". United States. https://www.osti.gov/servlets/purl/879580.
@article{osti_879580,
title = {Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.},
author = {Alfano, Robert R and Wang, Wubao},
abstractNote = {A method and system for examining biological materials using low-power cw excitation Raman spectroscopy. In accordance with the teachings of the invention, a low-power continuous wave (cw) pump laser beam and a low-power cw Stokes (or anti-Stokes) probe laser beam simultaneously illuminate a biological material and traverse the biological material in collinearity. The pump beam, whose frequency is varied, is used to induce Raman emission from the biological material. The intensity of the probe beam, whose frequency is kept constant, is monitored as it leaves the biological material. When the difference between the pump and probe excitation frequencies is equal to a Raman vibrational mode frequency of the biological material, the weak probe signal becomes amplified by one or more orders of magnitude (typically up to about 10.sup.4 -10.sup.6) due to the Raman emission from the pump beam. In this manner, by monitoring the intensity of the probe beam emitted from the biological material as the pump beam is varied in frequency, one can obtain an excitation Raman spectrum for the biological material tested. The present invention may be applied to in the in vivo and/or in vitro diagnosis of diabetes, heart disease, hepatitis, cancers and other diseases by measuring the characteristic excitation Raman lines of blood glucose, cholesterol, serum glutamic oxalacetic transaminase (SGOT)/serum glutamic pyruvic tansaminase (SGPT), tissues and other corresponding Raman-active body constituents, respectively. For example, it may also be used to diagnose diseases associated with the concentration of Raman-active constituents in urine, lymph and saliva It may be used to identify cancer in the breast, cervix, uterus, ovaries and the like by measuring the fingerprint excitation Raman spectra of these tissues. It may also be used to reveal the growing of tumors or cancers by measuring the levels of nitric oxide in tissue.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Nov 21 00:00:00 EST 2000},
month = {Tue Nov 21 00:00:00 EST 2000}
}