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Title: Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.

Abstract

A method and system for examining biological materials using low-power cw excitation Raman spectroscopy. A low-power continuous wave (cw) pump laser beam and a low-power cw Stokes (or anti-Stokes) probe laser beam simultaneously illuminate a biological material and traverse the biological material in collinearity. The pump beam, whose frequency is varied, is used to induce Raman emission from the biological material. The intensity of the probe beam, whose frequency is kept constant, is monitored as it leaves the biological material. When the difference between the pump and probe excitation frequencies is equal to a Raman vibrational mode frequency of the biological material, the weak probe signal becomes amplified by one or more orders of magnitude (typically up to about 10.sup.4 -10.sup.6) due to the Raman emission from the pump beam. In this manner, by monitoring the intensity of the probe beam emitted from the biological material as the pump beam is varied in frequency, one can obtain an excitation Raman spectrum for the biological material tested. The present invention may be applied to in the in vivo and/or in vitro diagnosis of diabetes, heart disease, hepatitis, cancers and other diseases by measuring the characteristic excitation Raman lines of blood glucose,more » cholesterol, serum glutamic oxalacetic transaminase (SGOT)/serum glutamic pyruvic transaminase (SGPT), tissues and other corresponding Raman-active body constituents, respectively.« less

Inventors:
 [1];  [2]
  1. Bronx, NY
  2. Flushing, NY
Issue Date:
Research Org.:
The Research Foundation of City University of New York (New York, NY)
OSTI Identifier:
879677
Patent Number(s):
6560478
Application Number:
09/717327
Assignee:
The Research Foundation of City University of New York (New York, NY)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT
A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61B - DIAGNOSIS
DOE Contract Number:  
FG02-98ER62560
Resource Type:
Patent
Country of Publication:
United States
Language:
English

Citation Formats

Alfano, Robert R, and Wang, Wubao. Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.. United States: N. p., 2003. Web.
Alfano, Robert R, & Wang, Wubao. Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.. United States.
Alfano, Robert R, and Wang, Wubao. Tue . "Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.". United States. https://www.osti.gov/servlets/purl/879677.
@article{osti_879677,
title = {Method And System For Examining Biological Materials Using Low Power Cw Excitation Raman Spectroscopy.},
author = {Alfano, Robert R and Wang, Wubao},
abstractNote = {A method and system for examining biological materials using low-power cw excitation Raman spectroscopy. A low-power continuous wave (cw) pump laser beam and a low-power cw Stokes (or anti-Stokes) probe laser beam simultaneously illuminate a biological material and traverse the biological material in collinearity. The pump beam, whose frequency is varied, is used to induce Raman emission from the biological material. The intensity of the probe beam, whose frequency is kept constant, is monitored as it leaves the biological material. When the difference between the pump and probe excitation frequencies is equal to a Raman vibrational mode frequency of the biological material, the weak probe signal becomes amplified by one or more orders of magnitude (typically up to about 10.sup.4 -10.sup.6) due to the Raman emission from the pump beam. In this manner, by monitoring the intensity of the probe beam emitted from the biological material as the pump beam is varied in frequency, one can obtain an excitation Raman spectrum for the biological material tested. The present invention may be applied to in the in vivo and/or in vitro diagnosis of diabetes, heart disease, hepatitis, cancers and other diseases by measuring the characteristic excitation Raman lines of blood glucose, cholesterol, serum glutamic oxalacetic transaminase (SGOT)/serum glutamic pyruvic transaminase (SGPT), tissues and other corresponding Raman-active body constituents, respectively.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {5}
}

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