Microscopy imaging system and method employing stimulated raman spectroscopy as a contrast mechanism

Title: Microscopy imaging system and method employing stimulated raman spectroscopy as a contrast mechanism

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Abstract

A microscopy imaging system includes a first light source for providing a first train of pulses at a first center optical frequency .omega..sub.1, a second light source for providing a second train of pulses at a second center optical frequency .omega..sub.2, a modulator system, an optical detector, and a processor. The modulator system is for modulating a beam property of the second train of pulses at a modulation frequency f of at least 100 kHz. The optical detector is for detecting an integrated intensity of substantially all optical frequency components of the first train of pulses from the common focal volume by blocking the second train of pulses being modulated. The processor is for detecting, a modulation at the modulation frequency f, of the integrated intensity of the optical frequency components of the first train of pulses to provide a pixel of an image for the microscopy imaging system.

Inventors:

Xie, Xiaoliang Sunney ^[1]; Freudiger, Christian ^[2]; Min, Wei ^[3]

Lexington, MA
Boston, MA
Cambridge, MA

Issue Date:: 2011-09-27

Research Org.:: President & Fellows of Harvard College (Cambridge, MA)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1027112

Patent Number(s):: 8027032

Application Number:: 12/196,746

Assignee:: President & Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):: G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT

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G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2021/655 - {Stimulated Raman}

G - PHYSICS G01 - MEASURING G01J - MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRA-RED, VISIBLE OR ULTRA-VIOLET LIGHT
G01J3/44 - Raman spectrometry

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
G01N2021/656 - {Raman microprobe}
G01N2201/08 - Optical fibres
G01N2021/653 - {Coherent methods [CARS]}
G01N21/65 - Raman scattering

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DOE Contract Number:: FG02-07ER15875

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 47 OTHER INSTRUMENTATION

Citation Formats


                    Xie, Xiaoliang Sunney, Freudiger, Christian, and Min, Wei. Microscopy imaging system and method employing stimulated raman spectroscopy as a contrast mechanism.  United States: N. p., 2011. 
        Web.

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                    Xie, Xiaoliang Sunney, Freudiger, Christian, & Min, Wei. Microscopy imaging system and method employing stimulated raman spectroscopy as a contrast mechanism.  United States.

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                    Xie, Xiaoliang Sunney, Freudiger, Christian, and Min, Wei. Tue .  
        "Microscopy imaging system and method employing stimulated raman spectroscopy as a contrast mechanism".  United States.  https://www.osti.gov/servlets/purl/1027112.

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@article{osti_1027112,

  title        = {Microscopy imaging system and method employing stimulated raman spectroscopy as a contrast mechanism},

  author       = {Xie, Xiaoliang Sunney and Freudiger, Christian and Min, Wei},

  abstractNote = {A microscopy imaging system includes a first light source for providing a first train of pulses at a first center optical frequency .omega..sub.1, a second light source for providing a second train of pulses at a second center optical frequency .omega..sub.2, a modulator system, an optical detector, and a processor. The modulator system is for modulating a beam property of the second train of pulses at a modulation frequency f of at least 100 kHz. The optical detector is for detecting an integrated intensity of substantially all optical frequency components of the first train of pulses from the common focal volume by blocking the second train of pulses being modulated. The processor is for detecting, a modulation at the modulation frequency f, of the integrated intensity of the optical frequency components of the first train of pulses to provide a pixel of an image for the microscopy imaging system.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2011},

  month        = {9}

}

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