Cytochrome b5 gene and protein of Candida tropicalis and methods relating thereto
Abstract
A novel gene has been isolated which encodes cytochrome b5 (CYTb5) protein of the .omega.-hydroxylase complex of C. tropicalis 20336. Vectors including this gene, and transformed host cells are provided. Methods of increasing the production of a CYTb5 protein are also provided which involve transforming a host cell with a gene encoding this protein and culturing the cells. Methods of increasing the production of a dicarboxylic acid are also provided which involve increasing in the host cell the number of genes encoding this protein.
- Inventors:
-
- Fort Thomas, KY
- Westfield, IN
- Cincinnati, OH
- Issue Date:
- Research Org.:
- GE CORPORATE R & D
- OSTI Identifier:
- 874978
- Patent Number(s):
- 6503734
- Assignee:
- Cognis Corporation (Gulph Mills, PA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
- DOE Contract Number:
- FC36-95GO10099
- Resource Type:
- Patent
- Country of Publication:
- United States
- Language:
- English
- Subject:
- cytochrome; b5; protein; candida; tropicalis; methods; relating; novel; isolated; encodes; cytb5; omega-hydroxylase; complex; 20336; vectors; including; transformed; host; cells; provided; increasing; production; involve; transforming; cell; encoding; culturing; dicarboxylic; acid; genes; host cell; /435/999/
Citation Formats
Craft, David L, Madduri, Krishna M, and Loper, John C. Cytochrome b5 gene and protein of Candida tropicalis and methods relating thereto. United States: N. p., 2003.
Web.
Craft, David L, Madduri, Krishna M, & Loper, John C. Cytochrome b5 gene and protein of Candida tropicalis and methods relating thereto. United States.
Craft, David L, Madduri, Krishna M, and Loper, John C. Wed .
"Cytochrome b5 gene and protein of Candida tropicalis and methods relating thereto". United States. https://www.osti.gov/servlets/purl/874978.
@article{osti_874978,
title = {Cytochrome b5 gene and protein of Candida tropicalis and methods relating thereto},
author = {Craft, David L and Madduri, Krishna M and Loper, John C},
abstractNote = {A novel gene has been isolated which encodes cytochrome b5 (CYTb5) protein of the .omega.-hydroxylase complex of C. tropicalis 20336. Vectors including this gene, and transformed host cells are provided. Methods of increasing the production of a CYTb5 protein are also provided which involve transforming a host cell with a gene encoding this protein and culturing the cells. Methods of increasing the production of a dicarboxylic acid are also provided which involve increasing in the host cell the number of genes encoding this protein.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Wed Jan 01 00:00:00 EST 2003},
month = {Wed Jan 01 00:00:00 EST 2003}
}
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Works referenced in this record:
Genetic Analysis of Cytochrome b5 from Arachidonic Acid-Producing Fungus, Mortierella alpina 1S-4: Cloning, RNA Editing and Expression of the Gene in Escherichia coli, and Purification and Characterization of the Gene Product
journal, June 1999
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journal, December 1982
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Cloning and characterization of a yeast cytochrome b5-encoding gene which suppresses ketoconazole hypersensitivity in a NADPH-P-450 reductase-deficient strain
journal, May 1994
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