Methods for detecting and determining levels of monolignol ester conjugates incorporated into lignin and compounds relating thereto

Ralph, John; Karlen, Steven D.; Lu, Fachuang; Padmakshan, Dharshana

Title: Methods for detecting and determining levels of monolignol ester conjugates incorporated into lignin and compounds relating thereto

Patent · Tue Aug 16 00:00:00 EDT 2022

OSTI ID:1924983

Ralph, John; Karlen, Steven D.; Lu, Fachuang; Padmakshan, Dharshana

Methods of detecting and, optionally, determining a level of incorporation of monolignol ester conjugates into lignin. The methods include derivatizing lignin to acylate at least a portion of free phenolic and aliphatic hydroxyls and to halogenate at least a portion of benzylic alcohols present in the lignin to yield derivatized lignin, treating the derivatized lignin with a reducing agent to cleave at least a portion of the derivatized lignin to yield lignin cleavage products, acetylating at least a portion of free hydroxyl groups in the lignin cleavage products with a labeled acetylation agent to yield labeled lignin fragments, and detecting the labeled lignin fragments.

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Research Organization:: Univ. of Wisconsin, Madison, WI (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FC02-07ER64494

Assignee:: Wisconsin Alumni Research Foundation (Madison, WI)

Patent Number(s):: 11,414,372

Application Number:: 16/252,915

OSTI ID:: 1924983

Resource Relation:: Patent File Date: 01/21/2019

Country of Publication:: United States

Language:: English

References (42)

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