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Title: Production of a highly active, soluble form of the cytochrome P450 reductase (CPR A) from Candida tropicalis

Abstract

The present invention provides soluble cytochrome p450 reductase (CPR) proteins from Candida sp. having an altered N-terminal region which results in reduced hydrophobicity of the N-terminal region. Also provided are host cells comprising the subject soluble CPR proteins. In addition, the present invention provides nucleotide and corresponding amino acid sequences for soluble CPR proteins and vectors comprising the nucleotide sequences. Methods for producing a soluble CPR, for increasing production of a dicarboxylic acid, and for detecting a cytochrome P450 are also provided.

Inventors:
Issue Date:
Research Org.:
Cognis Corporation, Ambler, PA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175854
Patent Number(s):
7083960
Application Number:
10/272,017
Assignee:
Cognis Corporation (Ambler, PA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
W-31-109-ENG-38
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES

Citation Formats

Donnelly, Mark. Production of a highly active, soluble form of the cytochrome P450 reductase (CPR A) from Candida tropicalis. United States: N. p., 2006. Web.
Donnelly, Mark. Production of a highly active, soluble form of the cytochrome P450 reductase (CPR A) from Candida tropicalis. United States.
Donnelly, Mark. Tue . "Production of a highly active, soluble form of the cytochrome P450 reductase (CPR A) from Candida tropicalis". United States. https://www.osti.gov/servlets/purl/1175854.
@article{osti_1175854,
title = {Production of a highly active, soluble form of the cytochrome P450 reductase (CPR A) from Candida tropicalis},
author = {Donnelly, Mark},
abstractNote = {The present invention provides soluble cytochrome p450 reductase (CPR) proteins from Candida sp. having an altered N-terminal region which results in reduced hydrophobicity of the N-terminal region. Also provided are host cells comprising the subject soluble CPR proteins. In addition, the present invention provides nucleotide and corresponding amino acid sequences for soluble CPR proteins and vectors comprising the nucleotide sequences. Methods for producing a soluble CPR, for increasing production of a dicarboxylic acid, and for detecting a cytochrome P450 are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2006},
month = {8}
}

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Works referenced in this record:

Generation of a Complete, Soluble, and Catalytically Active Sterol 14α-Demethylase−Reductase Complex
journal, July 1999


Truncations at the NH 2 Terminus of Rhodanese Destabilize the Enzyme and Decrease Its Heterologous Expression
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A 36-Residue Peptide Contains All of the Information Required for 7B2-mediated Activation of Prohormone Convertase 2
journal, July 1999


Dissection of NADPH-cytochrome P450 oxidoreductase into distinct functional domains.
journal, August 1994


Activities and Kinetic Mechanisms of Native and Soluble NADPH–Cytochrome P450 Reductase
journal, August 2001