Production of a highly active, soluble form of the cytochrome P450 reductase (CPR A) from Candida tropicalis
Abstract
The present invention provides soluble cytochrome p450 reductase (CPR) proteins from Candida sp. having an altered N-terminal region which results in reduced hydrophobicity of the N-terminal region. Also provided are host cells comprising the subject soluble CPR proteins. In addition, the present invention provides nucleotide and corresponding amino acid sequences for soluble CPR proteins and vectors comprising the nucleotide sequences. Methods for producing a soluble CPR, for increasing production of a dicarboxylic acid, and for detecting a cytochrome P450 are also provided.
- Inventors:
- Issue Date:
- Research Org.:
- Cognis Corporation, Ambler, PA (United States); Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1175854
- Patent Number(s):
- 7083960
- Application Number:
- 10/272,017
- Assignee:
- Cognis Corporation (Ambler, PA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- W-31-109-ENG-38
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2002 Oct 15
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES
Citation Formats
Donnelly, Mark. Production of a highly active, soluble form of the cytochrome P450 reductase (CPR A) from Candida tropicalis. United States: N. p., 2006.
Web.
Donnelly, Mark. Production of a highly active, soluble form of the cytochrome P450 reductase (CPR A) from Candida tropicalis. United States.
Donnelly, Mark. Tue .
"Production of a highly active, soluble form of the cytochrome P450 reductase (CPR A) from Candida tropicalis". United States. https://www.osti.gov/servlets/purl/1175854.
@article{osti_1175854,
title = {Production of a highly active, soluble form of the cytochrome P450 reductase (CPR A) from Candida tropicalis},
author = {Donnelly, Mark},
abstractNote = {The present invention provides soluble cytochrome p450 reductase (CPR) proteins from Candida sp. having an altered N-terminal region which results in reduced hydrophobicity of the N-terminal region. Also provided are host cells comprising the subject soluble CPR proteins. In addition, the present invention provides nucleotide and corresponding amino acid sequences for soluble CPR proteins and vectors comprising the nucleotide sequences. Methods for producing a soluble CPR, for increasing production of a dicarboxylic acid, and for detecting a cytochrome P450 are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 01 00:00:00 EDT 2006},
month = {Tue Aug 01 00:00:00 EDT 2006}
}
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