Title: Lignases and aldo-keto reductases for conversion of lignin-containing materials to fermentable products

Abstract

Termites have specialized digestive systems that overcome the lignin barrier in wood to release fermentable simple sugars. Using the termite Reticulitermes flavipes and its gut symbionts, high-throughput titanium pyrosequencing and proteomics approaches experimentally compared the effects of lignin-containing diets on host-symbiont digestome composition. Proteomic investigations and functional digestive studies with recombinant lignocellulases conducted in parallel provided strong evidence of congruence at the transcription and translational levels and provide enzymatic strategies for overcoming recalcitrant lignin barriers in biofuel feedstocks. Briefly described, therefore, the disclosure provides a system for generating a fermentable product from a lignified plant material, the system comprising a cooperating series of at least two catalytically active polypeptides, where said catalytically active polypeptides are selected from the group consisting of: cellulase Cell-1, $$\beta$$-glu cellulase, an aldo-keto-reductase, a catalase, a laccase, and an endo-xylanase.

Inventors:

Scharf, Michael; Sethi, Amit

Issue Date:

2016-09-13

Research Org.:

Purdue Univ., West Lafayette, IN (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1324695

Patent Number(s):

9441256

Application Number:

14/379,990

Assignee:

Purdue Research Foundation (West Lafayette, IN)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N9/0006 - {acting on CH-OH groups as donors
C12N9/0008 - {acting on the aldehyde or oxo group of donors
C12N9/0061 - {Laccase
C12N9/0065 - {acting on hydrogen peroxide as acceptor
C12N9/2437 - {Cellulases
C12N9/2445 - {Beta-glucosidase
C12N9/2482 - {Endo-1,4-beta-xylanase

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P19/14 - produced by the action of a carbohydrase {

C - CHEMISTRY C12 - BIOCHEMISTRY C12Y - ENZYMES

C12Y111/01006 - Catalase

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y02 - TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE Y02P - CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS

Y02P20/52 - using catalysts, e.g. selective catalysts

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DOE Contract Number:  

FC03-97ER62402

Resource Type:

Patent

Resource Relation:

Patent File Date: 2013 Feb 11

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS