Cytochrome P450 BM3 enzyme variants for preparation of cyclopropanes
Abstract
The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
- Inventors:
- Issue Date:
- Research Org.:
- California Institute of Technology (CalTech), Pasadena, CA (United States)
- Sponsoring Org.:
- USDOE; National Institutes of Health (NIH)
- OSTI Identifier:
- 1823962
- Patent Number(s):
- 11008596
- Application Number:
- 16/246,246
- Assignee:
- California Institute of Technology (Pasadena, CA)
- DOE Contract Number:
- EB015846; FG02-06ER15762
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 01/11/2019
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Coelho, Pedro S., Brustad, Eric M., Arnold, Frances H., Wang, Zhan, and Lewis, Jared C. Cytochrome P450 BM3 enzyme variants for preparation of cyclopropanes. United States: N. p., 2021.
Web.
Coelho, Pedro S., Brustad, Eric M., Arnold, Frances H., Wang, Zhan, & Lewis, Jared C. Cytochrome P450 BM3 enzyme variants for preparation of cyclopropanes. United States.
Coelho, Pedro S., Brustad, Eric M., Arnold, Frances H., Wang, Zhan, and Lewis, Jared C. Tue .
"Cytochrome P450 BM3 enzyme variants for preparation of cyclopropanes". United States. https://www.osti.gov/servlets/purl/1823962.
@article{osti_1823962,
title = {Cytochrome P450 BM3 enzyme variants for preparation of cyclopropanes},
author = {Coelho, Pedro S. and Brustad, Eric M. and Arnold, Frances H. and Wang, Zhan and Lewis, Jared C.},
abstractNote = {The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2021},
month = {5}
}