Automated methods for scalable, parallelized enzymatic biopolymer synthesis and modification using microfluidic devices
Abstract
Methods for the automated template-free synthesis of user-defined sequence controlled biopolymers using microfluidic devices are described. The methods facilitate simultaneous synthesis of up to thousands of uniquely addressed biopolymers from the controlled movement and combination of regents as fluid droplets using microfluidic and EWOD-based systems. In some forms, biopolymers including nucleic acids, peptides, carbohydrates, and lipids are synthesized from step-wise assembly of building blocks based on a user-defined sequence of droplet movements. In some forms, the methods synthesize uniquely addressed nucleic acids of up to 1,000 nucleotides in length. Methods for adding, removing and changing barcodes on biopolymers are also provided. Biopolymers synthesized according to the methods, and libraries and databases thereof are also described. Modified biopolymers, including chemically modified nucleotides and biopolymers conjugated to other molecules are described.
- Inventors:
- Issue Date:
- Research Org.:
- Massachusetts Inst. of Technology (MIT), Cambridge, MA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); US Office of Naval Research (ONR); National Science Foundation (NSF)
- OSTI Identifier:
- 2294023
- Patent Number(s):
- 11851651
- Application Number:
- 16/012,583
- Assignee:
- Massachusetts Institute of Technology (Cambridge, MA)
- DOE Contract Number:
- SC0001088; CCF-1564025; N00014-16-121953; N00014-17-1-2609
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 06/19/2018
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Banal, James, Berleant, Joseph Don, Shepherd, Tyson, and Bathe, Mark. Automated methods for scalable, parallelized enzymatic biopolymer synthesis and modification using microfluidic devices. United States: N. p., 2023.
Web.
Banal, James, Berleant, Joseph Don, Shepherd, Tyson, & Bathe, Mark. Automated methods for scalable, parallelized enzymatic biopolymer synthesis and modification using microfluidic devices. United States.
Banal, James, Berleant, Joseph Don, Shepherd, Tyson, and Bathe, Mark. Tue .
"Automated methods for scalable, parallelized enzymatic biopolymer synthesis and modification using microfluidic devices". United States. https://www.osti.gov/servlets/purl/2294023.
@article{osti_2294023,
title = {Automated methods for scalable, parallelized enzymatic biopolymer synthesis and modification using microfluidic devices},
author = {Banal, James and Berleant, Joseph Don and Shepherd, Tyson and Bathe, Mark},
abstractNote = {Methods for the automated template-free synthesis of user-defined sequence controlled biopolymers using microfluidic devices are described. The methods facilitate simultaneous synthesis of up to thousands of uniquely addressed biopolymers from the controlled movement and combination of regents as fluid droplets using microfluidic and EWOD-based systems. In some forms, biopolymers including nucleic acids, peptides, carbohydrates, and lipids are synthesized from step-wise assembly of building blocks based on a user-defined sequence of droplet movements. In some forms, the methods synthesize uniquely addressed nucleic acids of up to 1,000 nucleotides in length. Methods for adding, removing and changing barcodes on biopolymers are also provided. Biopolymers synthesized according to the methods, and libraries and databases thereof are also described. Modified biopolymers, including chemically modified nucleotides and biopolymers conjugated to other molecules are described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 26 00:00:00 EST 2023},
month = {Tue Dec 26 00:00:00 EST 2023}
}
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