skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Microfluidic devices, systems, and methods for quantifying particles using centrifugal force

Abstract

Embodiments of the present invention are directed toward microfluidic systems, apparatus, and methods for measuring a quantity of cells in a fluid. Examples include a differential white blood cell measurement using a centrifugal microfluidic system. A method may include introducing a fluid sample containing a quantity of cells into a microfluidic channel defined in part by a substrate. The quantity of cells may be transported toward a detection region defined in part by the substrate, wherein the detection region contains a density media, and wherein the density media has a density lower than a density of the cells and higher than a density of the fluid sample. The substrate may be spun such that at least a portion of the quantity of cells are transported through the density media. Signals may be detected from label moieties affixed to the cells.

Inventors:
; ;
Issue Date:
Research Org.:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1226547
Patent Number(s):
9186668
Application Number:
12/891,956
Assignee:
Sandia Corporation (Albuquerque, NM)
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
AC04-94AL85000
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Sep 28
Country of Publication:
United States
Language:
English
Subject:
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS

Citation Formats

Schaff, Ulrich Y., Sommer, Gregory J., and Singh, Anup K. Microfluidic devices, systems, and methods for quantifying particles using centrifugal force. United States: N. p., 2015. Web.
Schaff, Ulrich Y., Sommer, Gregory J., & Singh, Anup K. Microfluidic devices, systems, and methods for quantifying particles using centrifugal force. United States.
Schaff, Ulrich Y., Sommer, Gregory J., and Singh, Anup K. Tue . "Microfluidic devices, systems, and methods for quantifying particles using centrifugal force". United States. https://www.osti.gov/servlets/purl/1226547.
@article{osti_1226547,
title = {Microfluidic devices, systems, and methods for quantifying particles using centrifugal force},
author = {Schaff, Ulrich Y. and Sommer, Gregory J. and Singh, Anup K.},
abstractNote = {Embodiments of the present invention are directed toward microfluidic systems, apparatus, and methods for measuring a quantity of cells in a fluid. Examples include a differential white blood cell measurement using a centrifugal microfluidic system. A method may include introducing a fluid sample containing a quantity of cells into a microfluidic channel defined in part by a substrate. The quantity of cells may be transported toward a detection region defined in part by the substrate, wherein the detection region contains a density media, and wherein the density media has a density lower than a density of the cells and higher than a density of the fluid sample. The substrate may be spun such that at least a portion of the quantity of cells are transported through the density media. Signals may be detected from label moieties affixed to the cells.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {11}
}

Patent:

Save / Share:

Works referenced in this record:

A B Cell-Based Sensor for Rapid Identification of Pathogens
journal, July 2003


Lab On A CD
journal, August 2006


Bead-based microfluidic immunoassays: The next generation
journal, February 2007


Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
journal, January 2007


Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
journal, January 2009


A fully automated immunoassay from whole blood on a disc
journal, January 2009


Infrared controlled waxes for liquid handling and storage on a CD-microfluidic platform
journal, January 2011


How Hofmeister ion interactions affect protein stability
journal, October 1996


Cell-Free DNA—A Marker to Predict Ischemic Brain Damage in a Rat Stroke Experimental Model
journal, January 2011


A molecular approach to bioseparations: Protein–protein and protein–salt interactions
journal, February 2006


Measurement of Circulating Cell-Free DNA Levels by a New Simple Fluorescent Test in Patients With Primary Colorectal Cancer
journal, February 2011


A rapid direct fluorescent assay for cell-free DNA quantification in biological fluids
journal, September 2009


Functional integration of DNA purification and concentration into a real time micro-PCR chip
journal, January 2011


Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients
journal, April 2006


Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms
journal, February 2006


Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation
journal, March 2011


A new biodosimetric method: branched DNA-based quantitative detection of B1 DNA in mouse plasma
journal, August 2010


Circulating DNA: a new diagnostic gold mine?
journal, October 2002


Staphylococcal Enterotoxin B as a Biological Weapon: Recognition, Management, and Surveillance of Staphylococcal Enterotoxin
journal, September 2006


Micro freef-low IEF enhanced by active cooling and functionalized gels
journal, December 2006


Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates
journal, March 2005


Parallel Nanoliter Microfluidic Analysis System
journal, June 2007


One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST)
journal, January 2011


Shiga toxin 1 acting on DNA in vitro is a heat-stable enzyme not requiring proteolytic activation
journal, April 2004


Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood
journal, January 2011


Cell-Free Plasma DNA as a Predictor of Outcome in Severe Sepsis and Septic Shock
journal, April 2008


Bioinformatic processing to identify single nucleotide polymorphism that potentially affect Ape1 function
journal, June 2011


Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection
journal, January 2007