Global to push GA events into
skip to main content

Title: Microfluidic devices, systems, and methods for quantifying particles using centrifugal force

Embodiments of the present invention are directed toward microfluidic systems, apparatus, and methods for measuring a quantity of cells in a fluid. Examples include a differential white blood cell measurement using a centrifugal microfluidic system. A method may include introducing a fluid sample containing a quantity of cells into a microfluidic channel defined in part by a substrate. The quantity of cells may be transported toward a detection region defined in part by the substrate, wherein the detection region contains a density media, and wherein the density media has a density lower than a density of the cells and higher than a density of the fluid sample. The substrate may be spun such that at least a portion of the quantity of cells are transported through the density media. Signals may be detected from label moieties affixed to the cells.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1226547
Assignee:
Sandia Corporation (Albuquerque, NM) SNL-A
Patent Number(s):
9,186,668
Application Number:
12/891,956
Contract Number:
AC04-94AL85000
Resource Relation:
Patent File Date: 2010 Sep 28
Research Org:
Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
72 PHYSICS OF ELEMENTARY PARTICLES AND FIELDS

Other works cited in this record:

Multistation, Single Channel Analytical Photometer and Method Of Use
patent, January 1971

Loading disk for photometric analyzer of rotary cuvette type
patent, July 1973

Separation of solid and liquid components of mixtures
patent, November 1978

Apparatus and method for measuring white blood cell and platelet concentrations in blood
patent, May 1979

Heterogeneous binding assay
patent, April 1987

Method and apparatus for measuring blood constituent counts
patent, July 1987

Method for separating the cellular components of blood samples
patent, July 1989

Method of separation employing magnetic particles and second medium
patent, January 1994

Assay of blood or other biologic samples for target analytes
patent, June 1997

DNA purification and isolation using magnetic particles
patent, January 1998

Assay system and method for conducting assays
patent, March 1999

Centrifugal hematology disposable
patent, November 2000

Devices and methods for using centripetal acceleration to drive fluid movement in a microfluidics system
patent, November 2001

Diagnostic tests and kits for Clostridium difficile
patent, January 2003

Monolithic microfluidic concentrators and mixers
patent, May 2005

Class characterization of circulating cancer cells isolated from body fluids and methods of use
patent, November 2005

Multi-parameter assays including analysis discs and methods relating thereto
patent, April 2006

Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto
patent, January 2007

Microfluidic particle-analysis systems
patent, December 2007

Centrifugal force based microfluidic device, microfluidic system including the same, and method of determining home position of the microfluidic device
patent, July 2010

A B Cell-Based Sensor for Rapid Identification of Pathogens
journal, July 2003
  • Rider, Todd H.; Petrovick, Martha S.; Nargi, Frances E.
  • Science, Vol. 301, Issue 5630, p. 213-215
  • DOI: 10.1126/science.1084920

Bead-based microfluidic immunoassays: The next generation
journal, February 2007

Comparison of sample fixation and the use of LDS-751 or anti-CD45 for leukocyte identification in mouse whole blood for flow cytometry
journal, January 2007
  • Maes, Melissa L.; Davidson, Lisa B.; McDonagh, Paul F.
  • Journal of Immunological Methods, Vol. 319, Issue 1-2, p. 79-86
  • DOI: 10.1016/j.jim.2006.10.016

Leukocyte analysis and differentiation using high speed microfluidic single cell impedance cytometry
journal, January 2009
  • Holmes, David; Pettigrew, David; Reccius, Christian H.
  • Lab on a Chip, Vol. 9, Issue 20, p. 2881-2889
  • DOI: 10.1039/B910053A

A fully automated immunoassay from whole blood on a disc
journal, January 2009
  • Lee, Beom Seok; Lee, Jung-Nam; Park, Jong-Myeon
  • Lab on a Chip, Vol. 9, Issue 11, p. 1548-1555
  • DOI: 10.1039/B820321K

Infrared controlled waxes for liquid handling and storage on a CD-microfluidic platform
journal, January 2011
  • Abi-Samra, Kameel; Hanson, Ryan; Madou, Marc
  • Lab on a Chip, Vol. 11, Issue 4, p. 723-726
  • DOI: 10.1039/C0LC00160K

How Hofmeister ion interactions affect protein stability
journal, October 1996

Cell-Free DNA—A Marker to Predict Ischemic Brain Damage in a Rat Stroke Experimental Model
journal, January 2011
  • Boyko, Matthew; Ohayon, Sharon; Goldsmith, Tomer
  • Journal of Neurosurgical Anesthesiology, Vol. 23, Issue 3, p. 222-228
  • DOI: 10.1097/ANA.0b013e31821b536a

A molecular approach to bioseparations: Protein–protein and protein–salt interactions
journal, February 2006

Measurement of Circulating Cell-Free DNA Levels by a New Simple Fluorescent Test in Patients With Primary Colorectal Cancer
journal, February 2011
  • Czeiger, David; Shaked, Gad; Eini, Hadar
  • American Journal of Clinical Pathology, Vol. 135, Issue 2, p. 264-270
  • DOI: 10.1309/AJCP4RK2IHVKTTZV

Functional integration of DNA purification and concentration into a real time micro-PCR chip
journal, January 2011
  • Min, Junhong; Kim, Joon-Ho; Lee, Youngsun
  • Lab on a Chip, Vol. 11, Issue 2, p. 259-265
  • DOI: 10.1039/C0LC00320D

Plasma DNA concentration as a predictor of mortality and sepsis in critically ill patients
journal, April 2006
  • Rhodes, Andrew; Wort, Stephen J.; Thomas, Helen
  • Critical Care, Vol. 10, Issue 2, Article No. R60
  • DOI: 10.1186/cc4894

Read-out concepts for multiplexed bead-based fluorescence immunoassays on centrifugal microfluidic platforms
journal, February 2006
  • Riegger, L.; Grumann, M.; Nann, T.
  • Sensors and Actuators A: Physical, Vol. 126, Issue 2, p. 455-462
  • DOI: 10.1016/j.sna.2005.11.006

Whole Blood Immunoassay Based on Centrifugal Bead Sedimentation
journal, March 2011

A new biodosimetric method: branched DNA-based quantitative detection of B1 DNA in mouse plasma
journal, August 2010
  • Zhang, L.; Zhang, M.; Yang, S.
  • The British Journal of Radiology, Vol. 83, Issue 992, p. 694-701
  • DOI: 10.1259/bjr/49886569

Circulating DNA: a new diagnostic gold mine?
journal, October 2002
  • Ziegler, Annemarie; Zangemeister-Wittke, Uwe; Stahel, Rolf A.
  • Cancer Treatment Reviews, Vol. 28, Issue 5, p. 255-271
  • DOI: 10.1016/S0305-7372(02)00077-4

Micro freef-low IEF enhanced by active cooling and functionalized gels
journal, December 2006
  • Albrecht, Jacob W.; Jensen, Klavs F.
  • ELECTROPHORESIS, Vol. 27, Issue 24, p. 4960-4969
  • DOI: 10.1002/elps.200600436

Ricin A-Chain Activity on Stem−Loop and Unstructured DNA Substrates
journal, March 2005
  • Amukele, Tim K.; Roday, Setu; Schramm, Vern L.
  • Biochemistry, Vol. 44, Issue 11, p. 4416-4425
  • DOI: 10.1021/bi0474362

Parallel Nanoliter Microfluidic Analysis System
journal, June 2007
  • Andersson, Per; Jesson, Gerald; Kylberg, Gunnar
  • Analytical Chemistry, Vol. 79, Issue 11, p. 4022-4030
  • DOI: 10.1021/ac061692y

One-step purification of nucleic acid for gene expression analysis via Immiscible Filtration Assisted by Surface Tension (IFAST)
journal, January 2011
  • Berry, Scott M.; Alarid, Elaine T.; Beebe, David J.
  • Lab on a Chip, Vol. 11, Issue 10, p. 1747-1753
  • DOI: 10.1039/C1LC00004G

Shiga toxin 1 acting on DNA in vitro is a heat-stable enzyme not requiring proteolytic activation
journal, April 2004
  • Brigotti, Maurizio; Carnicelli, Domenica; Vara, Antonio González
  • Biochimie, Vol. 86, Issue 4-5, p. 305-309
  • DOI: 10.1016/j.biochi.2004.03.009

Depurination of A4256 in 28 S rRNA by the Ribosome-inactivating Proteins from Barley and Ricin Results in Different Ribosome Conformations
journal, May 1996
  • Holmberg, Lovisa; Nygård, Odd
  • Journal of Molecular Biology, Vol. 259, Issue 1, p. 81-94
  • DOI: 10.1006/jmbi.1996.0303

Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood
journal, January 2011
  • Lee, Beom Seok; Lee, Yang Ui; Kim, Han-Sang
  • Lab on a Chip, Vol. 11, Issue 1, p. 70-78
  • DOI: 10.1039/C0LC00205D

Cell-Free Plasma DNA as a Predictor of Outcome in Severe Sepsis and Septic Shock
journal, April 2008

Characterization of EBV-genome negative “null” and “T” cell lines derived from children with acute lymphoblastic leukemia and leukemic transformed non-Hodgkin lymphoma
journal, May 1977
  • Schneider, Ulrich; Schwenk, Hans-Ulrich; Bornkamm, Georg
  • International Journal of Cancer, Vol. 19, Issue 5, p. 621-626
  • DOI: 10.1002/ijc.2910190505

Bioinformatic processing to identify single nucleotide polymorphism that potentially affect Ape1 function
journal, June 2011
  • Yu, Eizadora T.; Hadi, Masood Z.
  • Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 722, Issue 2, p. 140-146
  • DOI: 10.1016/j.mrgentox.2010.06.015

Polyethyleneimine functionalized iron oxide nanoparticles as agents for DNA delivery and transfection
journal, January 2007
  • McBain, S. C.; Yiu, H. H. P.; El Haj, A.
  • Journal of Materials Chemistry, Vol. 17, Issue 24, p. 2561-2565
  • DOI: 10.1039/B617402G

Similar records in DOepatents and OSTI.GOV collections: