RNA-guided nucleic acid modifying enzymes and methods of use thereof

Doudna, Jennifer A.; Banfield, Jillian; Burstein, David; Harrington, Lucas Benjamin; Strutt, Steven C.

Title: RNA-guided nucleic acid modifying enzymes and methods of use thereof

Abstract

The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.

Inventors:: Doudna, Jennifer A.; Banfield, Jillian; Burstein, David; Harrington, Lucas Benjamin; Strutt, Steven C.

Issue Date:: Tue Oct 24 00:00:00 EDT 2023

Research Org.:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE; National Science Foundation (NSF)

OSTI Identifier:: 2293753

Patent Number(s):: 11795472

Application Number:: 16/422,824

Assignee:: The Regents of the University of California (Oakland, CA)

DOE Contract Number:: 1244557; AC02-05CH11231

Resource Type:: Patent

Resource Relation:: Patent File Date: 05/24/2019

Country of Publication:: United States

Language:: English

Citation Formats


                    Doudna, Jennifer A., Banfield, Jillian, Burstein, David, Harrington, Lucas Benjamin, and Strutt, Steven C. RNA-guided nucleic acid modifying enzymes and methods of use thereof.  United States: N. p., 2023. 
        Web.

Copy to clipboard


                    Doudna, Jennifer A., Banfield, Jillian, Burstein, David, Harrington, Lucas Benjamin, & Strutt, Steven C. RNA-guided nucleic acid modifying enzymes and methods of use thereof.  United States.

Copy to clipboard


                    Doudna, Jennifer A., Banfield, Jillian, Burstein, David, Harrington, Lucas Benjamin, and Strutt, Steven C. Tue .  
        "RNA-guided nucleic acid modifying enzymes and methods of use thereof".  United States.  https://www.osti.gov/servlets/purl/2293753.

Copy to clipboard


                    
@article{osti_2293753,

  title        = {RNA-guided nucleic acid modifying enzymes and methods of use thereof},

  author       = {Doudna, Jennifer A. and Banfield, Jillian and Burstein, David and Harrington, Lucas Benjamin and Strutt, Steven C.},

  abstractNote = {The present disclosure provides CasX proteins, nucleic acids encoding the CasX proteins, and modified host cells comprising the CasX proteins and/or nucleic acids encoding same. CasX proteins are useful in a variety of applications, which are provided. The present disclosure provides CasX guide RNAs that bind to and provide sequence specificity to the CasX proteins, nucleic acids encoding the CasX guide RNAs, and modified host cells comprising the CasX guide RNAs and/or nucleic acids encoding same. CasX guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding same, as well as their associated archaeal Cas9 guide RNAs and nucleic acids encoding same.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {Tue Oct 24 00:00:00 EDT 2023},

  month        = {Tue Oct 24 00:00:00 EDT 2023}

}

Copy to clipboard

Patent:

Save / Share:

Export Metadata

Save to My Library

Works referenced in this record:

An updated evolutionary classification of CRISPR–Cas systems
journal, September 2015

Makarova, Kira S.; Wolf, Yuri I.; Alkhnbashi, Omer S.
Nature Reviews Microbiology, Vol. 13, Issue 11, p. 722-736
https://doi.org/10.1038/nrmicro3569

SnapShot: Class 2 CRISPR-Cas Systems
journal, January 2017

Makarova, Kira S.; Zhang, Feng; Koonin, Eugene V.
Cell, Vol. 168, Issue 1-2
https://doi.org/10.1016/j.cell.2016.12.038

Biology and Applications of CRISPR Systems: Harnessing Nature’s Toolbox for Genome Engineering
journal, January 2016

Wright, Addison V.; Nuñez, James K.; Doudna, Jennifer A.
Cell, Vol. 164, Issue 1-2
https://doi.org/10.1016/j.cell.2015.12.035

CRISPR enzymes and systems
patent, October 2017

Zhang, Feng; Zetsche, Bernd; Slaymaker, Ian
US Patent Document 9,790,490
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/9790490

Origins and evolution of CRISPR-Cas systems
journal, March 2019

Koonin, Eugene V.; Makarova, Kira S.
Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 374, Issue 1772
https://doi.org/10.1098/rstb.2018.0087