RNA-guided nucleic acid modifying enzymes and methods of use thereof

Doudna, Jennifer A.; Banfield, Jillian F.; Burstein, David; Harrington, Lucas Benjamin

Title: RNA-guided nucleic acid modifying enzymes and methods of use thereof

Abstract

The present disclosure provides CasY proteins, nucleic acids encoding the CasY proteins, and modified host cells comprising the CasY proteins and/or nucleic acids encoding same. CasY proteins are useful in a variety of applications, which are provided. The present disclosure provides CasY guide RNAs that bind to and provide sequence specificity to the CasY proteins, nucleic acids encoding the CasY guide RNAs, and modified host cells comprising the CasY guide RNAs and/or nucleic acids encoding same. CasY guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides methods of identifying a CRISPR RNA-guided endonuclease.

Inventors:: Doudna, Jennifer A.; Banfield, Jillian F.; Burstein, David; Harrington, Lucas Benjamin

Issue Date:: 2022-06-28

Research Org.:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Org.:: USDOE; National Science Foundation (NSF)

OSTI Identifier:: 1893077

Patent Number(s):: 11371062

Application Number:: 16/335,630

Assignee:: The Regents of the University of California (Oakland, CA)

Patent Classifications (CPCs):: A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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A - HUMAN NECESSITIES A61 - MEDICAL OR VETERINARY SCIENCE A61K - PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES
A61K38/46 - Hydrolases

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/113 - Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides
C12N15/902 - {using homologous recombination}
C12N2310/20 - involving clustered regularly interspaced short palindromic repeats [CRISPRs]
C12N2740/16043 - viral genome or elements thereof as genetic vector
C12N2750/14143 - viral genome or elements thereof as genetic vector
C12N9/22 - Ribonucleases {RNAses, DNAses

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DOE Contract Number:: 1244557; AC02-05CH11231

Resource Type:: Patent

Resource Relation:: Patent File Date: 09/28/2017

Country of Publication:: United States

Language:: English

Citation Formats


                    Doudna, Jennifer A., Banfield, Jillian F., Burstein, David, and Harrington, Lucas Benjamin. RNA-guided nucleic acid modifying enzymes and methods of use thereof.  United States: N. p., 2022. 
        Web.

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                    Doudna, Jennifer A., Banfield, Jillian F., Burstein, David, & Harrington, Lucas Benjamin. RNA-guided nucleic acid modifying enzymes and methods of use thereof.  United States.

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                    Doudna, Jennifer A., Banfield, Jillian F., Burstein, David, and Harrington, Lucas Benjamin. Tue .  
        "RNA-guided nucleic acid modifying enzymes and methods of use thereof".  United States.  https://www.osti.gov/servlets/purl/1893077.

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@article{osti_1893077,

  title        = {RNA-guided nucleic acid modifying enzymes and methods of use thereof},

  author       = {Doudna, Jennifer A. and Banfield, Jillian F. and Burstein, David and Harrington, Lucas Benjamin},

  abstractNote = {The present disclosure provides CasY proteins, nucleic acids encoding the CasY proteins, and modified host cells comprising the CasY proteins and/or nucleic acids encoding same. CasY proteins are useful in a variety of applications, which are provided. The present disclosure provides CasY guide RNAs that bind to and provide sequence specificity to the CasY proteins, nucleic acids encoding the CasY guide RNAs, and modified host cells comprising the CasY guide RNAs and/or nucleic acids encoding same. CasY guide RNAs are useful in a variety of applications, which are provided. The present disclosure provides methods of identifying a CRISPR RNA-guided endonuclease.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2022},

  month        = {6}

}

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Works referenced in this record:

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