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Title: DNA sequencing with pyrophosphatase

Abstract

A kit or solution is disclosed for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule and having a second single-stranded region homologous to the first single-stranded region. The first agent is able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture. The second agent is able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.

Inventors:
;
Issue Date:
Research Org.:
Harvard University
OSTI Identifier:
203883
Patent Number(s):
5,498,523
Application Number:
PAN: 8-275,339; CNN: Grant A1-06045
Assignee:
Harvard Coll., Cambridge, MA (United States)
DOE Contract Number:  
FG02-88ER60688
Resource Type:
Patent
Resource Relation:
Other Information: PBD: 12 Mar 1996
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; PHOSPHATASES; DNA SEQUENCING; DNA SEQUENCERS; DESIGN; MOLECULAR BIOLOGY; OLIGONUCLEOTIDES; MOLECULAR STRUCTURE

Citation Formats

Tabor, S, and Richardson, C C. DNA sequencing with pyrophosphatase. United States: N. p., 1996. Web.
Tabor, S, & Richardson, C C. DNA sequencing with pyrophosphatase. United States.
Tabor, S, and Richardson, C C. Tue . "DNA sequencing with pyrophosphatase". United States.
@article{osti_203883,
title = {DNA sequencing with pyrophosphatase},
author = {Tabor, S and Richardson, C C},
abstractNote = {A kit or solution is disclosed for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule and having a second single-stranded region homologous to the first single-stranded region. The first agent is able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture. The second agent is able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {3}
}