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Title: DNA sequencing with pyrophosphatase

A kit or solution is disclosed for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule and having a second single-stranded region homologous to the first single-stranded region. The first agent is able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture. The second agent is able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.
Inventors:
;
Issue Date:
OSTI Identifier:
203883
Assignee:
Harvard Coll., Cambridge, MA (United States) PTO; SCA: 550200; PA: EDB-96:061506; SN: 96001559464
Patent Number(s):
US 5,498,523/A/
Application Number:
PAN: 8-275,339; CNN: Grant A1-06045
Contract Number:
FG02-88ER60688
Resource Relation:
Other Information: PBD: 12 Mar 1996
Research Org:
Harvard University
Country of Publication:
United States
Language:
English
Subject:
55 BIOLOGY AND MEDICINE, BASIC STUDIES; PHOSPHATASES; DNA SEQUENCING; DNA SEQUENCERS; DESIGN; MOLECULAR BIOLOGY; OLIGONUCLEOTIDES; MOLECULAR STRUCTURE

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