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Title: DNA sequencing with pyrophosphatase

Abstract

A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.

Inventors:
 [1];  [2]
  1. Cambridge, MA
  2. Chestnut Hill, MA
Issue Date:
Research Org.:
Harvard University
OSTI Identifier:
870336
Patent Number(s):
5498523
Application Number:
08/275,339
Assignee:
President and Fellows of Harvard College (Cambridge, MA)
DOE Contract Number:  
FG02-88ER60688
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
dna; sequencing; pyrophosphatase; kit; solution; extension; oligonucleotide; primer; single-stranded; region; template; molecule; homologous; comprising; agent; reaction; mixture; reduce; amount; pyrophosphate; below; produced; absence; single-stranded region; reaction mixture; dna sequencing; template molecule; oligonucleotide primer; /435/436/

Citation Formats

Tabor, Stanley, and Richardson, Charles C. DNA sequencing with pyrophosphatase. United States: N. p., 1996. Web.
Tabor, Stanley, & Richardson, Charles C. DNA sequencing with pyrophosphatase. United States.
Tabor, Stanley, and Richardson, Charles C. Tue . "DNA sequencing with pyrophosphatase". United States. https://www.osti.gov/servlets/purl/870336.
@article{osti_870336,
title = {DNA sequencing with pyrophosphatase},
author = {Tabor, Stanley and Richardson, Charles C},
abstractNote = {A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {3}
}

Patent:

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