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Title: DNA sequencing with pyrophosphatase

A kit or solution for use in extension of an oligonucleotide primer having a first single-stranded region on a template molecule having a second single-stranded region homologous to the first single-stranded region, comprising a first agent able to cause extension of the first single-stranded region of the primer on the second single-stranded region of the template in a reaction mixture, and a second agent able to reduce the amount of pyrophosphate in the reaction mixture below the amount produced during the extension in the absence of the second agent.
Inventors:
 [1];  [2]
  1. (Cambridge, MA)
  2. (Chestnut Hill, MA)
Issue Date:
OSTI Identifier:
870336
Assignee:
President and Fellows of Harvard College (Cambridge, MA) CHO
Patent Number(s):
US 5498523
Application Number:
08/275,339
Contract Number:
FG02-88ER60688
Research Org:
Harvard University
Country of Publication:
United States
Language:
English
Subject:
dna; sequencing; pyrophosphatase; kit; solution; extension; oligonucleotide; primer; single-stranded; region; template; molecule; homologous; comprising; agent; reaction; mixture; reduce; amount; pyrophosphate; below; produced; absence; single-stranded region; reaction mixture; dna sequencing; template molecule; oligonucleotide primer; /435/436/

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