Title: Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites

Abstract

The present invention relates to genetically modified bacteria and methods of optimizing genetically modified bacteria for the production of a metabolite.

Inventors:

Church, George M.; Raman, Srivatsan; Taylor, Noah D.; Rogers, Jameson K.

Issue Date:

Tue Mar 14 00:00:00 EDT 2023

Research Org.:

Harvard Univ., Cambridge, MA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1987218

Patent Number(s):

11603528

Application Number:

16/718,736

Assignee:

President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors
C12N15/1034 - {Isolating an individual clone by screening libraries}
C12N15/1058 - {Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms}
C12N15/1079 - {Screening libraries by altering the phenotype or phenotypic trait of the host
C12N15/1086 - {Preparation or screening of expression libraries, e.g. reporter assays}
C12N15/52 - Genes encoding for enzymes or proenzymes
C12N15/63 - Introduction of foreign genetic material using vectors

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P1/04 - by using bacteria
C12P13/04 - Alpha- or beta- amino acids {
C12P13/14 - Glutamic acid
C12P13/227 - {Tryptophan}
C12P17/06 - containing a six-membered hetero ring, e.g. fluorescein
C12P17/08 - containing a hetero ring of at least seven ring members, e.g. zearalenone, macrolide aglycons
C12P17/18 - containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin {
C12P17/188 - {Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
C12P19/02 - Monosaccharides
C12P19/12 - Disaccharides
C12P19/42 - Cobalamins, i.e. vitamin B12, LLD factor
C12P19/44 - Preparation of O-glycosides, e.g. glucosides {
C12P29/00 - Preparation of compounds containing a naphthacene ring system, e.g. tetracycline
C12P33/00 - Preparation of steroids
C12P5/02 - acyclic {
C12P7/02 - containing a hydroxy group
C12P7/40 - containing a carboxyl group {including Peroxycarboxylic acids
C12P7/42 - Hydroxy-carboxylic acids
C12P7/44 - Polycarboxylic acids
C12P7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
C12P7/58 - Aldonic, ketoaldonic or saccharic acids

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DOE Contract Number:  

FG02-02ER63445

Resource Type:

Patent

Resource Relation:

Patent File Date: 12/18/2019

Country of Publication:

United States

Language:

English