Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites

Church, George M.; Raman, Srivatsan; Taylor, Noah D.; Rogers, Jameson

Title: Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites

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Abstract

The present invention relates to genetically modified bacteria and methods of optimizing genetically modified bacteria for the production of a metabolite.

Inventors:: Church, George M.; Raman, Srivatsan; Taylor, Noah D.; Rogers, Jameson

Issue Date:: 2020-02-04

Research Org.:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1632514

Patent Number(s):: 10550384

Application Number:: 14/775,025

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P1/04 - by using bacteria
C12P17/188 - {Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
C12P13/04 - Alpha- or beta- amino acids {
C12P7/58 - Aldonic, ketoaldonic or saccharic acids
C12P19/12 - Disaccharides
C12P13/14 - Glutamic acid

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/1079 - {Screening libraries by altering the phenotype or phenotypic trait of the host

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P17/08 - containing a hetero ring of at least seven ring members, e.g. zearalenone, macrolide aglycons
C12P17/06 - containing a six-membered hetero ring, e.g. fluorescein
C12P5/02 - acyclic {
C12P7/40 - containing a carboxyl group {including Peroxycarboxylic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/1034 - {Isolating an individual clone by screening libraries}

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P17/18 - containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin {

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors
C12N15/63 - Introduction of foreign genetic material using vectors
C12N15/52 - Genes encoding for enzymes or proenzymes

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/44 - Preparation of O-glycosides, e.g. glucosides {
C12P19/42 - Cobalamins, i.e. vitamin B12, LLD factor
C12P7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
C12P7/02 - containing a hydroxy group
C12P7/42 - Hydroxy-carboxylic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/1086 - {Preparation or screening of expression libraries, e.g. reporter assays}

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P33/00 - Preparation of steroids
C12P29/00 - Preparation of compounds containing a naphthacene ring system, e.g. tetracycline
C12P7/44 - Polycarboxylic acids
C12P19/02 - Monosaccharides

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/1058 - {Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms}

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P13/227 - {Tryptophan}

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DOE Contract Number:: FG02-02ER63445

Resource Type:: Patent

Resource Relation:: Patent File Date: 02/26/2014

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Church, George M., Raman, Srivatsan, Taylor, Noah D., and Rogers, Jameson. Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites.  United States: N. p., 2020. 
        Web.

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                    Church, George M., Raman, Srivatsan, Taylor, Noah D., & Rogers, Jameson. Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites.  United States.

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                    Church, George M., Raman, Srivatsan, Taylor, Noah D., and Rogers, Jameson. Tue .  
        "Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites".  United States.  https://www.osti.gov/servlets/purl/1632514.

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@article{osti_1632514,

  title        = {Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites},

  author       = {Church, George M. and Raman, Srivatsan and Taylor, Noah D. and Rogers, Jameson},

  abstractNote = {The present invention relates to genetically modified bacteria and methods of optimizing genetically modified bacteria for the production of a metabolite.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2020},

  month        = {2}

}

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