Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites

Title: Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites

Abstract

The present invention relates to genetically modified bacteria and methods of optimizing genetically modified bacteria for the production of a metabolite.

Inventors:: Church, George M.; Raman, Srivatsan; Taylor, Noah D.; Rogers, Jameson

Issue Date:: 2020-02-04

Research Org.:: Harvard Univ., Cambridge, MA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1632514

Patent Number(s):: 10550384

Application Number:: 14/775,025

Assignee:: President and Fellows of Harvard College (Cambridge, MA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P1/04 - by using bacteria
C12P17/188 - {Heterocyclic compound containing in the condensed system at least one hetero ring having nitrogen atoms and oxygen atoms as the only ring heteroatoms
C12P13/04 - Alpha- or beta- amino acids {
C12P7/58 - Aldonic, ketoaldonic or saccharic acids
C12P19/12 - Disaccharides
C12P13/14 - Glutamic acid

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/1079 - {Screening libraries by altering the phenotype or phenotypic trait of the host

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P17/08 - containing a hetero ring of at least seven ring members, e.g. zearalenone, macrolide aglycons
C12P17/06 - containing a six-membered hetero ring, e.g. fluorescein
C12P5/02 - acyclic {
C12P7/40 - containing a carboxyl group {including Peroxycarboxylic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/1034 - {Isolating an individual clone by screening libraries}

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P17/18 - containing at least two hetero rings condensed among themselves or condensed with a common carbocyclic ring system, e.g. rifamycin {

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N1/38 - Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors
C12N15/63 - Introduction of foreign genetic material using vectors
C12N15/52 - Genes encoding for enzymes or proenzymes

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P19/44 - Preparation of O-glycosides, e.g. glucosides {
C12P19/42 - Cobalamins, i.e. vitamin B12, LLD factor
C12P7/46 - Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
C12P7/02 - containing a hydroxy group
C12P7/42 - Hydroxy-carboxylic acids

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/1086 - {Preparation or screening of expression libraries, e.g. reporter assays}

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P33/00 - Preparation of steroids
C12P29/00 - Preparation of compounds containing a naphthacene ring system, e.g. tetracycline
C12P7/44 - Polycarboxylic acids
C12P19/02 - Monosaccharides

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/1058 - {Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms}

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P13/227 - {Tryptophan}

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DOE Contract Number:: FG02-02ER63445

Resource Type:: Patent

Resource Relation:: Patent File Date: 02/26/2014

Country of Publication:: United States

Language:: English

Citation Formats


                    Church, George M., Raman, Srivatsan, Taylor, Noah D., and Rogers, Jameson. Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites.  United States: N. p., 2020. 
        Web.

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                    Church, George M., Raman, Srivatsan, Taylor, Noah D., & Rogers, Jameson. Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites.  United States.

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                    Church, George M., Raman, Srivatsan, Taylor, Noah D., and Rogers, Jameson. Tue .  
        "Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites".  United States.  https://www.osti.gov/servlets/purl/1632514.

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@article{osti_1632514,

  title        = {Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites},

  author       = {Church, George M. and Raman, Srivatsan and Taylor, Noah D. and Rogers, Jameson},

  abstractNote = {The present invention relates to genetically modified bacteria and methods of optimizing genetically modified bacteria for the production of a metabolite.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2020},

  month        = {2}

}

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Works referenced in this record:

Growth differentiation factor promoter and uses therefor
patent, April 2003

Liang, Li-fang
US Patent Document 6,555,672
URL: http://patft.uspto.gov/netacgi/nph-Parser?patentnumber=6555672

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Title: Methods for selecting microbes from a diverse genetically modified library to detect and optimize the production of metabolites

Abstract

Citation Formats

Growth differentiation factor promoter and uses therefor patent, April 2003

Growth differentiation factor promoter and uses therefor
patent, April 2003