Production of hydroxylated fatty acids in genetically modified plants

Somerville, Chris; Broun, Pierre; van de Loo, Frank; Boddupalli, Sekhar S.

Title: Production of hydroxylated fatty acids in genetically modified plants

Patent · Tue Aug 30 00:00:00 EDT 2005

OSTI ID:1175477

Somerville, Chris; Broun, Pierre; van de Loo, Frank; Boddupalli, Sekhar S.

This invention relates to plant fatty acyl hydroxylases. Methods to use conserved amino acid or nucleotide sequences to obtain plant fatty acyl hydroxylases are described. Also described is the use of cDNA clones encoding a plant hydroxylase to produce a family of hydroxylated fatty acids in transgenic plants. In addition, the use of genes encoding fatty acid hydroxylases or desaturases to alter the level of lipid fatty acid unsaturation in transgenic plants is described.

View Patent

Cite

Export

Save

Research Organization:: Carnegie Inst. of Washington, Washington, DC (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: FG02-94ER20133

Assignee:: Carnegie Institution of Washington (Washington, DC)

Patent Number(s):: 6,936,728

Application Number:: 09/885,189

OSTI ID:: 1175477

Country of Publication:: United States

Language:: English

References (40)

Expression of the ?-subunit of ?-conglycinin in seeds of transgenic plants Bray, Elizabeth A.; Naito, Satoshi; Pan, Nai-Sui Planta, Vol. 172, Issue 3 https://doi.org/10.1007/BF00398665	journal	November 1987
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
Accumulation of Ricinoleic, Lesquerolic, and Densipolic Acids in Seeds of Transgenic Arabidopsis Plants That Express a Fatty Acyl Hydroxylase cDNA from Castor Bean Broun, P.; Somerville, C. Plant Physiology, Vol. 113, Issue 3 https://doi.org/10.1104/pp.113.3.933	journal	March 1997
The role of cytochrome b5 in Δ12 desaturation of oleic acid by microsomes of safflower (Carthamus tinctorius L.) Kearns, Ellen V.; Hugly, Suzanne; Somerville, Chris R. Archives of Biochemistry and Biophysics, Vol. 284, Issue 2 https://doi.org/10.1016/0003-9861(91)90319-E	journal	February 1991
Plasmid [omega]-3 Fatty Acid Desaturase cDNA from Ricinus communis van de Loo, F. J.; Somerville, C. Plant Physiology, Vol. 105, Issue 1 https://doi.org/10.1104/pp.105.1.443	journal	May 1994
Recent Studies of the Enzymic Synthesis of Ricinoleic Acid by Developing Castor Beans Moreau, Robert A.; Stumpf, Paul K. Plant Physiology, Vol. 67, Issue 4 https://doi.org/10.1104/pp.67.4.672	journal	April 1981
Arabidopsis FAD2 gene encodes the enzyme that is essential for polyunsaturated lipid synthesis. Okuley, J.; Lightner, J.; Feldmann, K. The Plant Cell, Vol. 6, Issue 1 https://doi.org/10.1105/tpc.6.1.147	journal	January 1994
Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Ditta, G.; Stanfield, S.; Corbin, D. Proceedings of the National Academy of Sciences, Vol. 77, Issue 12 https://doi.org/10.1073/pnas.77.12.7347	journal	December 1980
Use of the DNA polymerase chain reaction for homology probing: isolation of partial cDNA or genomic clones encoding the iron-sulfur protein of succinate dehydrogenase from several species. Gould, S. J.; Subramani, S.; Scheffler, I. E. Proceedings of the National Academy of Sciences, Vol. 86, Issue 6 https://doi.org/10.1073/pnas.86.6.1934	journal	March 1989
Seed development in Ricinus communis (castor bean). I. Descriptive morphology Greenwood, J. S.; Bewley, J. D. Canadian Journal of Botany, Vol. 60, Issue 9 https://doi.org/10.1139/b82-222	journal	September 1982
Analysis of oil and meal from Lesquerella fendleri seed Carlson, K. D.; Chaudhry, A.; Bagby, M. O. Journal of the American Oil Chemists' Society, Vol. 67, Issue 7 https://doi.org/10.1007/BF02638957	journal	July 1990
Evidence for cytochrome b5 as an electron donor in ricinoleic acid biosynthesis in microsomal preparations from developing castor bean (Ricinus communis L.) Smith, M. A.; Jonsson, L.; Stymne, S. Biochemical Journal, Vol. 287, Issue 1 https://doi.org/10.1042/bj2870141	journal	October 1992
Eight Histidine Residues Are Catalytically Essential in a Membrane-Associated Iron Enzyme, Stearoyl-CoA Desaturase, and Are Conserved in Alkane Hydroxylase and Xylene Monooxygenase Shanklin, John; Whittle, Edward; Fox, Brian G. Biochemistry, Vol. 33, Issue 43 https://doi.org/10.1021/bi00209a009	journal	January 1994
Glycerolipid Synthesis: Biochemistry and Regulation Browse, J.; Somerville, C. Annual Review of Plant Physiology and Plant Molecular Biology, Vol. 42, Issue 1 https://doi.org/10.1146/annurev.pp.42.060191.002343	journal	June 1991
Phospholipids of palash ( Butea monosperma ), papaya ( Carica papaya ), jangli badam ( Sterculia foetida ), coriander ( Coriandrum sativum ) and carrot ( Daucus carota ) seeds Prasad, R. B. N.; Rao, Y. Nagender; Rao, S. Venkob Journal of the American Oil Chemists' Society, Vol. 64, Issue 10 https://doi.org/10.1007/BF02636993	journal	October 1987
Characterization of two divergent β-tubulin genes from Colletotrichum graminicola Panaccione, Daniel G.; Hanau, Robert M. Gene, Vol. 86, Issue 2 https://doi.org/10.1016/0378-1119(90)90275-V	journal	February 1990
Modification of Plant Lipid Synthesis Topfer, R.; Martini, N.; Schell, J. Science, Vol. 268, Issue 5211 https://doi.org/10.1126/science.268.5211.681	journal	May 1995
Octopine Ti-plasmid deletion mutants of Agrobacterium tumefaciens with emphasis on the right side of the T-region Ooms, Gert; Hooykaas, Paul J. J.; Van Veen, Ron J. M. Plasmid, Vol. 7, Issue 1 https://doi.org/10.1016/0147-619X(82)90023-3	journal	January 1982
A bifunctional oleate 12‐hydroxylase: desaturase from Lesquerella fendleri Broun, Pierre; Boddupalli, Sekhar; Somerville, Chris The Plant Journal, Vol. 13, Issue 2 https://doi.org/10.1046/j.1365-313X.1998.00023.x	journal	January 1998
Signal sequences von Heijne, Gunnar Journal of Molecular Biology, Vol. 184, Issue 1 https://doi.org/10.1016/0022-2836(85)90046-4	journal	July 1985
[19] Isolation of multigene families and determination of homologies by filter hybridization methods Beltz, Gerald A.; Jacobs, Kenneth A.; Eickbush, Thomas H. Recombinant DNA Part B https://doi.org/10.1016/0076-6879(83)00061-0	book	January 1983
Formation of oil in the seed of Ricinus Communis l. Canvin, David T. Canadian Journal of Biochemistry and Physiology, Vol. 41, Issue 9 https://doi.org/10.1139/o63-214	journal	September 1963
The Biosynthesis of Ricinoleic acid James, At; Hadaway, Hc; Webb, Jpw Biochemical Journal, Vol. 95, Issue 2 https://doi.org/10.1042/bj0950448	journal	May 1965
Rapid isolation of high molecular weight plant DNA Murray, M. G.; Thompson, W. F. Nucleic Acids Research, Vol. 8, Issue 19 https://doi.org/10.1093/nar/8.19.4321	journal	January 1980
The mechanism of ricinoleic acid biosynthesis in Ricinuscommunis seeds Morris, L. J. Biochemical and Biophysical Research Communications, Vol. 29, Issue 3 https://doi.org/10.1016/0006-291X(67)90454-8	journal	November 1967
Occurrence of ricinoleic acid in submerged cultures of variousClaviceps sp. Křen, V.; Řezanka, T.; Řeháček, Z. Experientia, Vol. 41, Issue 11 https://doi.org/10.1007/BF01950045	journal	November 1985
Cloning of Higher Plant [omega]-3 Fatty Acid Desaturases Yadav, N. S.; Wierzbicki, A.; Aegerter, M. Plant Physiology, Vol. 103, Issue 2 https://doi.org/10.1104/pp.103.2.467	journal	October 1993
Structure and occurrence of unusual fatty acids in minor seed oils Badami, R.; Patil, K. Progress in Lipid Research, Vol. 19, Issue 3-4 https://doi.org/10.1016/0163-7827(80)90002-8	journal	January 1980
The specificity of fatty acid desaturases and hydroxylases Howling, D.; Morris, L. J.; Gurr, M. I. Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Vol. 260, Issue 1 https://doi.org/10.1016/0005-2760(72)90068-9	journal	January 1972
A Comparison of the Metabolic Fate of Fatty Acids of Different Chain Lengths in Developing Oilseeds Battey, James F.; Ohlrogge, John B. Plant Physiology, Vol. 90, Issue 3 https://doi.org/10.1104/pp.90.3.835	journal	July 1989
The promoter of TL-DNA gene 5 controls the tissue-specific expression of chimaeric genes carried by a novel type of Agrobacterium binary vector Koncz, Csaba; Schell, Jeff MGG Molecular & General Genetics, Vol. 204, Issue 3 https://doi.org/10.1007/BF00331014	journal	September 1986
The Biosynthesis of Ricinoleic Acid by Claviceps purpurea Morris, L. J.; Hall, S. W.; James, A. T. Biochemical Journal, Vol. 100, Issue 3 https://doi.org/10.1042/bj1000029C	journal	September 1966
No growth for NASA, NSF Jones, R. M.; Leath, A. T. Eos, Transactions American Geophysical Union, Vol. 73, Issue 28 https://doi.org/10.1029/91EO00234	journal	January 1992
Mechanisms and stereochemistry in fatty acid metabolism Morris, L. J. Biochemical Journal, Vol. 118, Issue 5 https://doi.org/10.1042/bj1180681g	journal	August 1970
DST sequences, highly conserved among plant SAUR genes, target reporter transcripts for rapid decay in tobacco. Newman, T. C.; Ohme-Takagi, M.; Taylor, C. B. The Plant Cell, Vol. 5, Issue 6 https://doi.org/10.1105/tpc.5.6.701	journal	June 1993
Primary structure of xylene monooxygenase: similarities to and differences from the alkane hydroxylation system. Suzuki, M.; Hayakawa, T.; Shaw, J. P. Journal of Bacteriology, Vol. 173, Issue 5 https://doi.org/10.1128/JB.173.5.1690-1695.1991	journal	January 1991
Map-based cloning of a gene controlling omega-3 fatty acid desaturation in Arabidopsis Arondel, V.; Lemieux, B.; Hwang, I. Science, Vol. 258, Issue 5086 https://doi.org/10.1126/science.1455229	journal	November 1992
Ricinoleic acid biosynthesis and triacylglycerol assembly in microsomal preparations from developing castor-bean (Ricinus communis) endosperm Bafor, M.; Smith, M. A.; Jonsson, L. Biochemical Journal, Vol. 280, Issue 2 https://doi.org/10.1042/bj2800507	journal	December 1991
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids Broun, Pierre; Shanklin, John; Whittle, Ed Science, Vol. 282, Issue 5392, p. 1315-1317 https://doi.org/10.1126/science.282.5392.1315	journal	November 1998
An oleate 12-hydroxylase from Ricinus communis L. is a fatty acyl desaturase homolog. van de Loo, F. J.; Broun, P.; Turner, S. Proceedings of the National Academy of Sciences, Vol. 92, Issue 15 https://doi.org/10.1073/pnas.92.15.6743	journal	July 1995

Similar Records

Production of hydroxylated fatty acids in genetically modified plants

Patent · Tue Aug 23 00:00:00 EDT 2011 · OSTI ID:1175477

Somerville, Chris; Broun, Pierre; van de Loo, Frank; +1 more

Plant fatty acid hydroxylases

Patent · Mon Jan 01 00:00:00 EST 2001 · OSTI ID:1175477

Somerville, Chris; Broun, Pierre; van de Loo, Frank

Production of hydroxylated fatty acids in genetically modified plants

Patent · Mon Jan 01 00:00:00 EST 2001 · OSTI ID:1175477

Somerville, Chris; Broun, Pierre; van de Loo, Frank

Related Subjects

60 APPLIED LIFE SCIENCES

Title: Production of hydroxylated fatty acids in genetically modified plants

Citation Formats

References (40)

Similar Records

Related Subjects