Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression
Abstract
The present invention provides for a system comprising (a) a first nucleic acid comprising a nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter comprising a repressor polypeptide binding site, and (b) a second nucleic acid comprising a nucleotide sequence encoding a repressor polypeptide having at least 70% amino acid identity with EilR, SmvR, KmrR, RcdA, or QacR; wherein expression of the nucleotide sequence of interest from the promoter is induced by the presence of a hydrophobic inducer, such as a hydrophobic cation inducer, such as a triarylmethane, acridine, phenazine, phenothiazine, or xanthene.
- Inventors:
- Issue Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1892815
- Patent Number(s):
- 11299519
- Application Number:
- 16/831,649
- Assignee:
- The Regents of the University of California (Oakland, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- AC02-05CH11231
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 03/26/2020
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Ruegg, Thomas L. Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression. United States: N. p., 2022.
Web.
Ruegg, Thomas L. Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression. United States.
Ruegg, Thomas L. Tue .
"Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression". United States. https://www.osti.gov/servlets/purl/1892815.
@article{osti_1892815,
title = {Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression},
author = {Ruegg, Thomas L.},
abstractNote = {The present invention provides for a system comprising (a) a first nucleic acid comprising a nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter comprising a repressor polypeptide binding site, and (b) a second nucleic acid comprising a nucleotide sequence encoding a repressor polypeptide having at least 70% amino acid identity with EilR, SmvR, KmrR, RcdA, or QacR; wherein expression of the nucleotide sequence of interest from the promoter is induced by the presence of a hydrophobic inducer, such as a hydrophobic cation inducer, such as a triarylmethane, acridine, phenazine, phenothiazine, or xanthene.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Apr 12 00:00:00 EDT 2022},
month = {Tue Apr 12 00:00:00 EDT 2022}
}
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