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Title: Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression

Abstract

The present invention provides for a system comprising (a) a first nucleic acid comprising a nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter comprising a repressor polypeptide binding site, and (b) a second nucleic acid comprising a nucleotide sequence encoding a repressor polypeptide having at least 70% amino acid identity with EilR, SmvR, KmrR, RcdA, or QacR; wherein expression of the nucleotide sequence of interest from the promoter is induced by the presence of a hydrophobic inducer, such as a hydrophobic cation inducer, such as a triarylmethane, acridine, phenazine, phenothiazine, or xanthene.

Inventors:
Issue Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1650847
Patent Number(s):
10640537
Application Number:
15/201,248
Assignee:
The Regents of the University of California (Oakland, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
AC02-05CH11231
Resource Type:
Patent
Resource Relation:
Patent File Date: 07/01/2016
Country of Publication:
United States
Language:
English

Citation Formats

Ruegg, Thomas L. Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression. United States: N. p., 2020. Web.
Ruegg, Thomas L. Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression. United States.
Ruegg, Thomas L. Tue . "Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression". United States. https://www.osti.gov/servlets/purl/1650847.
@article{osti_1650847,
title = {Host cells and methods using a repressor polypeptide and an inducible promoter for gene expression},
author = {Ruegg, Thomas L.},
abstractNote = {The present invention provides for a system comprising (a) a first nucleic acid comprising a nucleotide sequence encoding a nucleotide sequence of interest operatively linked to a promoter comprising a repressor polypeptide binding site, and (b) a second nucleic acid comprising a nucleotide sequence encoding a repressor polypeptide having at least 70% amino acid identity with EilR, SmvR, KmrR, RcdA, or QacR; wherein expression of the nucleotide sequence of interest from the promoter is induced by the presence of a hydrophobic inducer, such as a hydrophobic cation inducer, such as a triarylmethane, acridine, phenazine, phenothiazine, or xanthene.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2020},
month = {5}
}

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