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Title: Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species

Abstract

The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 2 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.

Inventors:
 [1];  [2]
  1. Richland, WA
  2. Pendleton, OR
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
OSTI Identifier:
875089
Patent Number(s):
6,528,636
Assignee:
Battelle Memorial Institute (Richland, WA) PNNL
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
promoter; sequence; 3-phosphoglycerate; kinase; lactic; acid-producing; fungus; rhizopus; oryzae; method; expressing; fungal; species; provides; clone; discovery; phosphoglycerate; filamentous; strain; isolated; constitutively; regulate; expression; carbohydrate; conditions; addition; design; integration; vector; transformation; foreign; lactic acid; promoter sequence; /999/435/

Citation Formats

Gao, Johnway, and Skeen, Rodney S. Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species. United States: N. p., 2003. Web.
Gao, Johnway, & Skeen, Rodney S. Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species. United States.
Gao, Johnway, and Skeen, Rodney S. Tue . "Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species". United States. https://www.osti.gov/servlets/purl/875089.
@article{osti_875089,
title = {Promoter sequence of 3-phosphoglycerate kinase gene 2 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species},
author = {Gao, Johnway and Skeen, Rodney S},
abstractNote = {The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 2 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2003},
month = {3}
}

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