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Title: Promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species

Abstract

The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 1 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.

Inventors:
 [1];  [2]
  1. Richland, WA
  2. Pendleton, OR
Issue Date:
Research Org.:
Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
874806
Patent Number(s):
6465635
Assignee:
Battelle Memorial Institute (Richland, WA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
DOE Contract Number:  
AC06-76RL01830
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
promoter; sequence; 3-phosphoglycerate; kinase; lactic; acid-producing; fungus; rhizopus; oryzae; method; expressing; fungal; species; provides; clone; discovery; phosphoglycerate; filamentous; strain; isolated; constitutively; regulate; expression; carbohydrate; conditions; addition; design; integration; vector; transformation; foreign; lactic acid; promoter sequence; /999/435/

Citation Formats

Gao, Johnway, and Skeen, Rodney S. Promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species. United States: N. p., 2002. Web.
Gao, Johnway, & Skeen, Rodney S. Promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species. United States.
Gao, Johnway, and Skeen, Rodney S. Tue . "Promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species". United States. https://www.osti.gov/servlets/purl/874806.
@article{osti_874806,
title = {Promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species},
author = {Gao, Johnway and Skeen, Rodney S},
abstractNote = {The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 1 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2002},
month = {10}
}

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Works referenced in this record:

Transformation of Filamentous Fungi with Plasmid DNA by Electroporation
journal, January 1989


Sequence of the Penicillium chrysogenum phosphoglycerate kinase gene
journal, January 1988


The primary structure of the Saccharomyces cerevisiae gene for 3-phosphogrycerate kinase
journal, January 1982


Efficient synthesis of enzymatically active calf chymosin in Saccharomyces cerevisiae
journal, September 1983


Analysis of the 3-phosphoglycerate kinase 2 promoter in Rhizopus niveus
journal, January 1995


Gene Transfer by Electroporation of Filamentous Fungi
book, June 1995


Cloning, expression and characterization of a cDNA encoding a lipase from Rhizopus delemar
journal, December 1991


Production of l-lactic acid by Rhizopus species
journal, July 1994


Enhanced production of l(+)-lactic acid from corn starch in a culture of Rhizopus oryzae using an air-lift bioreactor
journal, January 1997