DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Variant Enzymes

Abstract

Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.

Inventors:
; ; ; ; ; ; ; ; ;
Issue Date:
Research Org.:
DANISCO US INC., Palo Alto, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1482204
Patent Number(s):
10081802
Application Number:
14/909,007
Assignee:
DANISCO US INC. (Palo Alto, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
DOE Contract Number:  
FC36-08GO18078
Resource Type:
Patent
Resource Relation:
Patent File Date: 2014 Jul 24
Country of Publication:
United States
Language:
English

Citation Formats

Foukaraki, Maria, Hommes, Ronaldus Wilhelmus, Kaper, Thijs, Kelemen, Bradley R., Kralj, Slavko, Lantz, Suzanne E., Nikolaev, Igor, Van Der Kley, Wilhelmus, Van Lieshout, Johannes Franciscus Thomas, and Thans, Sander Van Stigt. Variant Enzymes. United States: N. p., 2018. Web.
Foukaraki, Maria, Hommes, Ronaldus Wilhelmus, Kaper, Thijs, Kelemen, Bradley R., Kralj, Slavko, Lantz, Suzanne E., Nikolaev, Igor, Van Der Kley, Wilhelmus, Van Lieshout, Johannes Franciscus Thomas, & Thans, Sander Van Stigt. Variant Enzymes. United States.
Foukaraki, Maria, Hommes, Ronaldus Wilhelmus, Kaper, Thijs, Kelemen, Bradley R., Kralj, Slavko, Lantz, Suzanne E., Nikolaev, Igor, Van Der Kley, Wilhelmus, Van Lieshout, Johannes Franciscus Thomas, and Thans, Sander Van Stigt. Tue . "Variant Enzymes". United States. https://www.osti.gov/servlets/purl/1482204.
@article{osti_1482204,
title = {Variant Enzymes},
author = {Foukaraki, Maria and Hommes, Ronaldus Wilhelmus and Kaper, Thijs and Kelemen, Bradley R. and Kralj, Slavko and Lantz, Suzanne E. and Nikolaev, Igor and Van Der Kley, Wilhelmus and Van Lieshout, Johannes Franciscus Thomas and Thans, Sander Van Stigt},
abstractNote = {Disclosed are glycosyl hydrolase enzyme variants, particularly variants of certain oxidoreductases of glycosyl hydrolase family 61. Nucleic acids encoding the glycosyl hydrolyase variants, compositions including the glycosyl hydrolase variants, methods of producing the variants, and methods of using the variants are also described.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2018},
month = {9}
}

Works referenced in this record:

Novel Glycosyl Hydrolase Enzymes and Uses Thereof
patent-application, July 2013


Heterologous Expression of Termite Cellulases in Yeast
patent-application, June 2016


Identification of a fungal cutinase promoter that is inducible by a plant signal via a phosphorylated trans-acting factor.
journal, September 1991


The Streptomyces reticuli α-chitin-binding protein CHB2 and its gene
journal, May 1998


Reshaping human antibodies: grafting an antilysozyme activity
journal, March 1988


Comparative Study of Two Chitin-Active and Two Cellulose-Active AA10-Type Lytic Polysaccharide Monooxygenases
journal, March 2014


Molecular cloning and characterization of the glucoamylase gene of Aspergillus awamori.
journal, November 1984


NMR structure of a lytic polysaccharide monooxygenase provides insight into copper binding, protein dynamics, and substrate interactions
journal, October 2012


CelS: a novel endoglucanase identified from Erwinia carotovora subsp. carotovora
journal, May 1990


Cloning and sequencing of cellulase cDNA from Aspergillus kawachii and its expression in Saccharomyces cerevisiae
journal, April 1995


Complete nucleotide sequence of a gene coding for Aspergillus aculeatus cellulase (FI-CMCase)
journal, January 1990


Characterization of the Chitinolytic Machinery of Enterococcus faecalis V583 and High-Resolution Structure of Its Oxidative CBM33 Enzyme
journal, February 2012


ACEII, a Novel Transcriptional Activator Involved in Regulation of Cellulase and Xylanase Genes of Trichoderma reesei
journal, April 2001


Composites Containing Polypeptides Attached to Polysaccharides and Molecules
patent-application, April 2011


Methods for Improving the Efficiency of Simultaneous Saccharification and Fermentation Reactions
patent-application, June 2013


Codon optimization of xylanase gene xynB from the thermophilic bacterium Dictyoglomus thermophilum for expression in the filamentous fungus Trichoderma reesei
journal, September 2000


Compositions Comprising a Polypeptide Having Cellulolytic Enhancing Activity and a Liquor and Uses Thereof
patent-application, March 2017


Integration of bacterial lytic polysaccharide monooxygenases into designer cellulosomes promotes enhanced cellulose degradation
journal, June 2014


Site saturation mutagenesis: Methods and applications in protein engineering
journal, July 2012


Purification and properties of an extracellular β-glucosidase from Lenzites trabea
journal, January 1978


Quantitative interpretations of double mutations of enzymes
journal, May 1992


Enzyme feed additive and animal feed including it
patent, May 2003


Fractionation of cellulase and β-glucosidase in a Trichoderma reesei culture liquid by use of two-phase partitioning
journal, November 1999


Cellulose-Degrading Enzyme Composition Comprising GH16
patent-application, November 2015


Properties of cellulolytic enzyme systems
journal, April 1985


Molecular Biology of Cellulolytic Fungi
book, January 1995


Structural diversity of lytic polysaccharide monooxygenases
journal, June 2017


Engineered Pentafunctional Minicellulosome for Simultaneous Saccharification and Ethanol Fermentation in Saccharomyces cerevisiae
journal, August 2014


Method for Reducing Viscosity in Saccharification Process
patent-application, April 2017


A Structural Overview of gh61 Proteins – Fungal Cellulose Degrading Polysaccharide Monooxygenases
journal, September 2012


Structure and boosting activity of a starch-degrading lytic polysaccharide monooxygenase
journal, January 2015


Chapter One - Heterologous Gene Expression in Filamentous Fungi
book, January 2012


Composites Containing Polypeptides Attached to Polysaccharides and Molecules
patent-application, April 2014


Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations.
journal, August 1983


Cloning and Sequencing of a Rhodothermus marinus Gene, bglA, Coding for a Thermostable beta-Glucanase and its Expression in Escherichia coli
journal, September 1994


GH61 Polypeptide Variants and Polynucleotides Encoding Same
patent-application, March 2015


Reshaping human antibodies for therapy
journal, March 1988


Transformation of Aspergillus nidulans by using a trpC plasmid.
journal, March 1984


Cloning and sequencing of the cDNA encoding β-glucosidase 1 from Aspergillus aculeatus
journal, September 1996


Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina)
journal, May 2008


Improved transformation efficiency of Aspergillus niger using the homologous niaD gene for nitrate reductase
journal, July 1989


Enzyme feed additive and animal feed including it
patent, May 2014


Improved oligonudeotide site-directed rautagenesis using M13 vectors
journal, January 1985


Efficient transformation of Dictyostelium discoideum amoebae.
journal, December 1983


[57] Rethinking your purification procedure
book, January 1990


Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975


Simultaneous saccharification and fermentation of lignocellulosic wastes to ethanol using a thermotolerant yeast
journal, April 2001


Basic local alignment search tool
journal, October 1990


Cloning, sequencing and overexpression of a Rhodothermus marinus gene encoding a thermostable cellulase of glycosyl hydrolase family 12
journal, March 1998


Comparison of biosequences
journal, December 1981


Microbial diversity of cellulose hydrolysis
journal, June 2011


A novel genetic system to detect protein–protein interactions
journal, July 1989


Structural and Functional Characterization of a Lytic Polysaccharide Monooxygenase with Broad Substrate Specificity
journal, July 2015


Synthesis and secretion of wheat α-amylase in Saccharomyces cerevisiae
journal, January 1987


Primary structure of the trpC gene from Aspergillus nidulans
journal, January 1985


Crystal structure of a bacterial family-III cellulose-binding domain: a general mechanism for attachment to cellulose.
journal, November 1996


Expansion of the enzymatic repertoire of the CAZy database to integrate auxiliary redox enzymes
journal, January 2013


Cellulase enzymes and systems for their expressions
patent, February 1999


Trichoderma reesei cellulases and their core domains in the hydrolysis and modification of chemical pulp
journal, June 2000


Chimeric proteins with a cellulose binding domain
patent, June 2002


Methods of Degrading or Hydrolyzing a Polysaccharide
patent-application, August 2013


Crystal Structure and Binding Properties of the Serratia marcescens Chitin-binding Protein CBP21
journal, December 2004


Variant Enzymes
patent-application, June 2016


Novel Cellobiohydrolase Enzymes
patent-application, February 2013


Construction of mutant and chimeric genes using the polymerase chain reaction
journal, January 1989


Metal Abstraction Peptide and Uses Thereof
patent-application, January 2013


Polypeptides Having Amylolytic Enhancing Activity and Polynucleotides Encoding Same
patent-application, November 2011


A New Model For Enzymatic Hydrolysis of Cellulose Based on the Two-Domain Structure of Cellobiohydrolase I
journal, March 1991


Structure of Cellulases and Their Applications
journal, April 1997


Expression of Aureobasidium pullulans xynA in, and secretion of the xylanase from, Saccharomyces cerevisiae.
journal, January 1996


Amino acid substitution matrices from protein blocks.
journal, November 1992


Re-annotation of the CAZy genes of Trichoderma reesei and transcription in the presence of lignocellulosic substrates
journal, January 2012


A versatile transformation system for the cellulolytic filamentous fungus Trichoderma reesei
journal, January 1987


Inverse Thinking about Double Mutants of Enzymes
journal, November 2004


Studies of the cellulolytic system of Trichoderma reesei QM 9414. Analysis of domain function in two cellobiohydrolases by limited proteolysis
journal, January 1988


Characteristics of fungal cellulases
journal, January 1991


Cassette mutagenesis: an efficient method for generation of multiple mutations at defined sites
journal, January 1985


Variant Enzymes
patent-application, June 2016


Kinetics of cellulose saccharification by Trichoderma reesei cellulases
journal, January 2001


Separation of endo- and exo-type cellulases using a new affinity chromatography method
journal, April 1984


Characterization of cellulases by HPLC separation
journal, January 1983


Methods and compositions for degrading cellulosic material
patent, September 2015


Method of Improving the Activity of Cellulase Enzyme Mixtures in the Saccharification (Ligno)Cellulosic Material
patent-application, October 2013


Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor: Investigation of Yields, Kinetics, and Enzymatic Digestibilities of Solids
journal, January 2003


Methods for reducing the harshness of a cotton-containing fabric
patent, July 1997


R: A Language for Data Analysis and Graphics
journal, September 1996


Enzyme Feed Additive and Animal Feed Including It
patent-application, August 2006


Purification and characterization of a β-glucosidase from solid-state cultures of Humicola grisea var. thermoidea
journal, January 1996


Structural and functional characterization of a conserved pair of bacterial cellulose-oxidizing lytic polysaccharide monooxygenases
journal, May 2014


Transformation of Trichoderma harzianum by high-voltage electric pulse
journal, February 1990


Method for Reducing Viscosity in Saccharification Process
patent-application, May 2014


Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor
book, January 2003


Molecular phylogeny and species delimitation in the section Longibrachiatum of Trichoderma
journal, May 2012


Antibodies specific for the human retinoblastoma protein identify a family of related polypeptides.
journal, November 1991


Variant Enzymes
patent-application, June 2016


Use of Aspergillus overproducing mutants, cured for intergrated plasmid, to overproduce heterologous proteins
journal, August 1993


GH61 Glycoside Hydrolase Protein Variants and Cofactors that Enhance GH61 Activity
patent-application, February 2013


Expression of two Trichoderma reesei endoglucanases in the yeast Saccharomyces cerevisiae
journal, September 1987


Discovery and characterization of a new family of lytic polysaccharide monooxygenases
journal, December 2013


Polypeptides having cellulolytic enhancing activity and polynucleotides encoding same
patent-application, January 2006


Gapped BLAST and PSI-BLAST: a new generation of protein database search programs
journal, September 1997


Variants of Polypeptides Having Cellulolytic Enhancing Activity and Polynucleotides Encoding Same
patent-application, August 2013


Engineered Secreted Proteins and Methods
patent-application, October 2015


GH61 Polypeptide Variants and Polynucleotides Encoding Same
patent-application, January 2015


Improved tools for biological sequence comparison.
journal, April 1988


Rapid and efficient site-specific mutagenesis without phenotypic selection.
journal, January 1985


Organization of a gene cluster expressed specifically in the asexual spores of A. nidulans
journal, October 1981


Production of Antigen-Specific Human Antibodies from Mice Engineered with Human Heavy and Light Chain YACs
journal, September 1995


Enzyme feed additive and animal feed including it
patent, February 2006


Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris: Optimizing Yield and N-terminal Processing
journal, August 2015


Limited proteolysis of the cellobiohydrolase I from Trichoderma reesei: Separation of functional domains
journal, August 1986


The First Structure of a Glycoside Hydrolase Family 61 Member, Cel61B from Hypocrea jecorina, at 1.6 Å Resolution
journal, October 2008


Importance of Hydrogen-Bond Formation in Stabilizing the Transition State of Subtilisin
journal, April 1986

  • Wells, J. A.; Cunningham, B. C.; Graycar, T. P.
  • Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 317, Issue 1540, p. 415-423
  • https://doi.org/10.1098/rsta.1986.0051

The Copper Active Site of CBM33 Polysaccharide Oxygenases
journal, April 2013


Adenoassociated Virus Vectors for the Treatment of Lysosomal Storage Disorders
patent-application, March 2017


Biolistic transformation of Trichoderma harzianum and Gliocladium virens using plasmid and genomic DNA
journal, October 1993


Method and Compositions for Treating Cellulose Containing Fabrics Using Truncated Cellulase Enzyme Compositions
patent-application, November 2002


Structural and Electronic Snapshots during the Transition from a Cu(II) to Cu(I) Metal Center of a Lytic Polysaccharide Monooxygenase by X-ray Photoreduction
journal, May 2014


abfB-2 Gene from Penicillium Funiculosum
patent-application, January 2010


Two different types of intervening sequences in the glucoamylase gene from Aspergillus niger.
journal, July 1984


[79] Purification of all glycolytic enzymes from one muscle extract
journal, January 1982


Structural Basis for Substrate Targeting and Catalysis by Fungal Polysaccharide Monooxygenases
journal, June 2012


Methods for Enhancing the Degradation or Conversion of Cellulosic Material
patent-application, July 2015


cDNA Cloning of a Trichoderma reesei Cellulase and Demonstration of Endoglucanase Activity by Expression in Yeast
journal, October 1997


Genetically engineered peptide fusions for improved protein partitioning in aqueous two-phase systems
journal, March 2001


EGIII, a new endoglucanase from Trichoderma reesei: the characterization of both gene and enzyme
journal, March 1988


R: A Language for Data Analysis and Graphics
journal, September 1996


A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970


Isolation and partial characterization of cellulose-degrading strain of Streptomyces sp. LX from soil
journal, March 1996


A comparison of gel-based, nylon filter and microarray techniques to detect differential RNA expression in plants
journal, April 1999


SCOP: A structural classification of proteins database for the investigation of sequences and structures
journal, April 1995


Methods for measuring cellulase activities
journal, January 1988


Cloning and relational analysis of 15 novel fungal endoglucanases from family 12 glycosyl hydrolase
journal, May 2002


Efficient secretion of two fungal cellobiohydrolases by Saccharomyces cerevisiae
journal, March 1988


Cellulase families and their genes
journal, September 1987


Replacing the complementarity-determining regions in a human antibody with those from a mouse
journal, May 1986


Homologous expression and characterization of Cel61A (EG IV) of Trichoderma reesei
journal, December 2001


Novel Glycosyl Hydrolase Enzymes and Uses Therof
patent-application, June 2016


Genetic transformation of intact cells of Bacillus subtilis by electroporation
journal, January 1990


Temperature Effects on Kinetic Parameters and Substrate Affinity of Cel7A Cellobiohydrolases
journal, July 2015


Glycosyl Hydrolase Enzymes and Uses Thereof for Biomass Hydrolysis
patent-application, April 2014


Activity Studies and Crystal Structures of Catalytically Deficient Mutants of Cellobiohydrolase I fromTrichoderma reesei
journal, November 1996


Viruses and gene silencing in plants
book, January 1999


Methods for enhancing the degradation or conversion of cellulosic material
patent-application, April 2007


Analytical separation of Trichoderma reesei cellulases by ion-exchange fast protein liquid chromatography
journal, January 1987


Cullulase Enzymes Having a Modified Linker and Reduced Lignin Binding
patent-application, July 2016


Cellulases of Trichoderma reesei
journal, January 1988


Characterization of the secreted cellulases of Trichoderma reesei wild type and mutants during controlled fermentations
journal, July 1984


Structural and Functional Analysis of a Lytic Polysaccharide Monooxygenase Important for Efficient Utilization of Chitin in Cellvibrio japonicus
journal, February 2016


CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994


Cloning and characterization of the ethanol utilization regulon in Aspergillus nidulans
journal, January 1985


Insights into the oxidative degradation of cellulose by a copper metalloenzyme that exploits biomass components
journal, August 2011


The Non-catalytic Chitin-binding Protein CBP21 from Serratia marcescens Is Essential for Chitin Degradation
journal, June 2005


Identification of a Genomic Region Containing a Novel Promoter Resistant to Glucose Repression and Over-Expression of β-Glucosidase Gene in Hypocrea orientalis EU7-22
journal, April 2013


Applications and statistics for multiple high-scoring segments in molecular sequences.
journal, June 1993


Methods and compositions for degrading cellulosic material
patent-application, May 2010


Secretion of Trichoderma reesei β-glucosidase by Saccharomyces cerevisiae
journal, February 1996


Structural basis for the enhancement of virulence by viral spindles and their in vivo crystallization
journal, March 2015


Enzymic activities of endo-1,4-β-d-glucanases purified from Trichoderma viride
journal, March 1978


Studies on the chromatographic fractionation of Trichoderma reesei cellulases by hydrophobic interaction
journal, December 1999


Detergent cellulase
patent, March 1984