Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes
Abstract
A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.
- Inventors:
- Issue Date:
- Research Org.:
- Idaho National Laboratory (INL), Idaho Falls, ID (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1243341
- Patent Number(s):
- 9290784
- Application Number:
- 14/185,501
- Assignee:
- Battelle Energy Alliance, LLC (Idaho Falls, ID)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AC07-99ID13727; AC07-05ID14517
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2014 Feb 20
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Thompson, David N., Apel, William A., Thompson, Vicki S., and Ward, Thomas E. Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes. United States: N. p., 2016.
Web.
Thompson, David N., Apel, William A., Thompson, Vicki S., & Ward, Thomas E. Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes. United States.
Thompson, David N., Apel, William A., Thompson, Vicki S., and Ward, Thomas E. Tue .
"Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes". United States. https://www.osti.gov/servlets/purl/1243341.
@article{osti_1243341,
title = {Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes},
author = {Thompson, David N. and Apel, William A. and Thompson, Vicki S. and Ward, Thomas E.},
abstractNote = {A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from Alicyclobacillus acidocaldarius and encoding a polypeptide involved in at least partially degrading, cleaving, transporting, metabolizing, or removing polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, xylan-, glucan-, galactan-, or mannan-decorating groups; and at least one nucleic acid sequence and/or at least one recombinant nucleic acid encoding a polypeptide involved in fermenting sugar molecules to a product. Additionally, enzymatic and/or proteinaceous extracts may be isolated from one or more genetically modified organisms. The extracts are utilized to convert biomass into a product. Further provided are methods of converting biomass into products comprising: placing the genetically modified organism and/or enzymatic extracts thereof in fluid contact with polysaccharides, cellulose, lignocellulose, hemicellulose, lignin, starch, sugars, sugar oligomers, carbohydrates, complex carbohydrates, chitin, heteroxylans, glycosides, and/or xylan-, glucan-, galactan-, or mannan-decorating groups.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Mar 22 00:00:00 EDT 2016},
month = {Tue Mar 22 00:00:00 EDT 2016}
}
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Works referenced in this record:
A new class of biodegradable materials: Poly-3-hydroxy-butyrate/steam exploded straw fiber composites. I. Thermal and impact behavior
journal, September 1993
- Avella, Maurizio; Martuscelli, Ezio; Pascucci, Beniamino
- Journal of Applied Polymer Science, Vol. 49, Issue 12, p. 2091-2103
Interlaboratory testing of methods for assay of xylanase activity
journal, May 1992
- Bailey, Michael J.; Biely, Peter; Poutanen, Kaisa
- Journal of Biotechnology, Vol. 23, Issue 3, p. 257-270
Molecular diversity of thermophilic cellulolytic and hemicellulolytic bacteria
journal, February 1999
- Bergquist, P.
- FEMS Microbiology Ecology, Vol. 28, Issue 2
Thermoacidophilic Microorganisms and their Novel Biocatalysts
journal, December 2004
- Bertoldo, C.; Dock, C.; Antranikian, G.
- Engineering in Life Sciences, Vol. 4, Issue 6, p. 521-532
Microbial β-Glucosidases: Cloning, Properties, and Applications
journal, January 2002
- Bhatia, Yukti; Mishra, Saroj; Bisaria, V. S.
- Critical Reviews in Biotechnology, Vol. 22, Issue 4, p. 375-407
Powers and Pitfalls in Sequence Analysis: The 70% Hurdle
journal, April 2000
- Bork, P.
- Genome Research, Vol. 10, Issue 4, p. 398-400
Deciphering the message in protein sequences: tolerance to amino acid substitutions
journal, March 1990
- Bowie, J.; Reidhaar-Olson, J.; Lim, W.
- Science, Vol. 247, Issue 4948, p. 1306-1310
Catalytic Plasticity of Fatty Acid Modification Enzymes Underlying Chemical Diversity of Plant Lipids
journal, November 1998
- Broun, Pierre; Shanklin, John; Whittle, Ed
- Science, Vol. 282, Issue 5392, p. 1315-1317
Yeast systems for the expression of heterologous gene products
journal, October 1993
- Buckholz, Richard G.
- Current Opinion in Biotechnology, Vol. 4, Issue 5, p. 538-542
Single molecule detection of RNA reporter probes by amplification with Qβ replicase
journal, August 1996
- Burg, Lawrence J.; Juffras, Ann Marie; Wu, Ye
- Molecular and Cellular Probes, Vol. 10, Issue 4, p. 257-271
Synthesis of an amplifiable reporter RNA for bioassays
journal, January 1986
- Chu, Barbara C. F.; Kramer, Fred Russell; Orgel, Leslie E.
- Nucleic Acids Research, Vol. 14, Issue 14, p. 5591-5603
Xylanases, xylanase families and extremophilic xylanases
journal, January 2005
- Collins, Tony; Gerday, Charles; Feller, Georges
- FEMS Microbiology Reviews, Vol. 29, Issue 1, p. 3-23
Glycosidases and glycosyl transferases in glycoside and oligosaccharide synthesis
journal, February 1998
- Crout, David H. G.; Vic, Gabin
- Current Opinion in Chemical Biology, Vol. 2, Issue 1, p. 98-111
Gene cloning, sequencing, and characterization of a family 9 endoglucanase (CelA) with an unusual pattern of activity from the thermoacidophile Alicyclobacillus acidocaldarius ATCC27009
journal, December 2002
- K., Eckert; F., Zielinski; Leggio, L. Lo
- Applied Microbiology and Biotechnology, Vol. 60, Issue 4, p. 428-436
Current applications of COS cell based transient expression systems
journal, October 1993
- Edwards, Caroline P.; Aruffo, Alejandro
- Current Opinion in Biotechnology, Vol. 4, Issue 5, p. 558-563
The nature of lignocellulosics and their pretreatments for enzymatic hydrolysis
book, January 1982
- Fan, L. T.; Lee, Young-Hyun; Gharpuray, M. M.
- Microbial Reactions, p. 157-187
Development of gas phase bioreactors for the removal of nitrogen oxides from synthetic flue gas streams
journal, October 2002
- Flanagan, William P.; Apel, William A.; Barnes, Joni M.
- Fuel, Vol. 81, Issue 15, p. 1953-1961
Crystallographic and mutational analyses of an extremely acidophilic and acid-stable xylanase: biased distribution of acidic residues and importance of Asp37 for catalysis at low pH
journal, December 1998
- Fushinobu, S.; Ito, K.; Konno, M.
- Protein Engineering Design and Selection, Vol. 11, Issue 12, p. 1121-1128
Manufacture of Xylose-Based Fermentation Media from Corncobs by Posthydrolysis of Autohydrolysis Liquors
journal, January 2001
- Garrote, Gil; DomÃnguez, Herminia; Parajó, Juan C.
- Applied Biochemistry and Biotechnology, Vol. 95, Issue 3, p. 195-208
Transformation of Acidiphilium by electroporation and conjugation
journal, May 1992
- Glenn, Anne W.; Roberto, Frank F.; Ward, Thomas E.
- Canadian Journal of Microbiology, Vol. 38, Issue 5, p. 387-393
Pretreatment for enhanced hydrolysis of cellulosic biomass
journal, January 1984
- Grethlein, Hans E.
- Biotechnology Advances, Vol. 2, Issue 1, p. 43-62
Comparison of the economics of acid and enzymatic hydrolysis of newsprint
journal, April 1978
- Grethlein, Hans E.
- Biotechnology and Bioengineering, Vol. 20, Issue 4, p. 503-525
Ethanol from lignocellulosic biomass techno-economic performance in short-, middle- and long-term
journal, April 2005
- Hamelinck, Carlo N; Hooijdonk, Geertje van; Faaij, André PC
- Biomass and Bioenergy, Vol. 28, Issue 4, p. 384-410
Fungal Upgrading of Wheat Straw for Straw-Thermoplastics Production
journal, January 2004
- Houghton, Tracy P.; Thompson, David N.; Hess, J. Richard
- Applied Biochemistry and Biotechnology, Vol. 113, Issue 1-3, p. 71-93
Maltose and Maltodextrin Transport in the Thermoacidophilic Gram-Positive Bacterium Alicyclobacillus acidocaldarius Is Mediated by a High-Affinity Transport System That Includes a Maltose Binding Protein Tolerant to Low pH
journal, November 2000
- Hülsmann, A.; Lurz, R.; Scheffel, F.
- Journal of Bacteriology, Vol. 182, Issue 22, p. 6292-6301
Immunogenicity and protective efficacy of a tuberculosis DNA vaccine
journal, August 1996
- Huygen, Kris; Content, Jean; Denis, Olivier
- Nature Medicine, Vol. 2, Issue 8, p. 893-898
Purification and Properties of Acid Stable Xylanases from Aspergillus kawachii
journal, January 1992
- Ito, Kiyoshi; Ogasawara, Hironobu; Sugimoto, Takashi
- Bioscience, Biotechnology, and Biochemistry, Vol. 56, Issue 4, p. 547-550
Biochemistry and genetics of microbial xylanases
journal, June 1996
- Jeffries, Thomas W.
- Current Opinion in Biotechnology, Vol. 7, Issue 3, p. 337-342
Cloning and transcriptional analysis of the Thermoanaerobacter ethanolicus strain 39E maltose ABC transport system
journal, March 2002
- Jones, Chris R.; Ray, Myrna; Strobel, Herbert J.
- Extremophiles, Vol. 6, Issue 4, p. 291-299
journal, October 2003
- Kenealy, William R.; Jeffries, Thomas W.
- Biotechnology Letters, Vol. 25, Issue 19, p. 1619-1623
Partial acid hydrolysis of cellulosic materials as a pretreatment for enzymatic hydrolysis
journal, July 1980
- Knappert, Diane; Grethlein, Hans; Converse, Alvin
- Biotechnology and Bioengineering, Vol. 22, Issue 7, p. 1449-1463
Continuous cultures of fused cells secreting antibody of predefined specificity
journal, August 1975
- Köhler, G.; Milstein, C.
- Nature, Vol. 256, Issue 5517, p. 495-497
Molecular and biotechnological aspects of xylanases
journal, July 1999
- Kulkarni, Neeta; Shendye, Abhay; Rao, Mala
- FEMS Microbiology Reviews, Vol. 23, Issue 4, p. 411-456
PCR ligation mutagenesis in transformable streptococci: application and efficiency
journal, April 2002
- Lau, Peter C. Y.; Sung, Chang Kyoo; Lee, Janet H.
- Journal of Microbiological Methods, Vol. 49, Issue 2, p. 193-205
Characterization of a β-glycosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius
journal, April 2006
- Lauro, Barbara Di; Rossi, Mosè; Moracci, Marco
- Extremophiles, Vol. 10, Issue 4, p. 301-310
Isolation and characterization of a new family 42 β-galactosidase from the thermoacidophilic bacterium Alicyclobacillus acidocaldarius: Identification of the active site residues
journal, February 2008
- Di Lauro, Barbara; Strazzulli, Andrea; Perugino, Giuseppe
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1784, Issue 2, p. 292-301
The acid hydrolysis of sugarcane bagasse hemicellulose to produce xylose, arabinose, glucose and other products
journal, November 2002
- Lavarack, B. P.; Griffin, G. J.; Rodman, D.
- Biomass and Bioenergy, Vol. 23, Issue 5, p. 367-380
Oxygen effects on thermophilic microbial populations in biofilters treating nitric oxide containing off-gas streams
journal, October 2001
- Lee, Brady D.; Apel, William A.; Smith, William A.
- Environmental Progress, Vol. 20, Issue 3, p. 157-166
The Effect of Flow Rate of Compressed Hot Water on Xylan, Lignin, and Total Mass Removal from Corn Stover
journal, September 2003
- Liu, Chaogang; Wyman, Charles E.
- Industrial & Engineering Chemistry Research, Vol. 42, Issue 21, p. 5409-5416
Baculovirus systems for the expression of human gene products
journal, October 1993
- Luckow, Verne A.
- Current Opinion in Biotechnology, Vol. 4, Issue 5, p. 564-572
Overview and Evaluation of Fuel Ethanol from Cellulosic Biomass: Technology, Economics, the Environment, and Policy
journal, November 1996
- Lynd, Lee R.
- Annual Review of Energy and the Environment, Vol. 21, Issue 1, p. 403-465
Lignocellulose biodegradation: Fundamentals and applications
journal, June 2002
- Malherbe, S.; Cloete, T. E.
- Reviews in Environmental Science and Bio/Technology, Vol. 1, Issue 2, p. 105-114
Analytical strategies for the use of DNA probes
journal, February 1988
- Matthews, Jayne A.; Kricka, Larry J.
- Analytical Biochemistry, Vol. 169, Issue 1, p. 1-25
The Synthesis of Bovine Insulin by the Solid Phase Method
journal, November 1966
- Marglin, B.; Merrifield, R. B.
- Journal of the American Chemical Society, Vol. 88, Issue 21, p. 5051-5052
Specificity of the protein secretory apparatus: secretion of the heat-labile enterotoxin B subunit pentamers by different species of Gram- bacteria
journal, January 1995
- Michel, Linda Overbye; Sandkvist, Maria; Bagdasarian, Michael
- Gene, Vol. 152, Issue 1, p. 41-45
Autocatalytic replication of a recombinant RNA
journal, December 1983
- Miele, Eleanor A.; Mills, Donald R.; Kramer, Fred Russell
- Journal of Molecular Biology, Vol. 171, Issue 3, p. 281-295
Industrial Scale-Up of pH-Controlled Liquid Hot Water Pretreatment of Corn Fiber for Fuel Ethanol Production
journal, January 2005
- Mosier, Nathan S.; Hendrickson, Richard; Brewer, Mark
- Applied Biochemistry and Biotechnology, Vol. 125, Issue 2, p. 77-98
A general method applicable to the search for similarities in the amino acid sequence of two proteins
journal, March 1970
- Needleman, Saul B.; Wunsch, Christian D.
- Journal of Molecular Biology, Vol. 48, Issue 3, p. 443-453
Purification and characterization of an acidophilic xylanase from Aureobasidium pullulans var. melanigenum and sequence analysis of the encoding gene
journal, January 2001
- Ohta, Kazuyoshi; Moriyama, Satoshi; Tanaka, Hidenori
- Journal of Bioscience and Bioengineering, Vol. 92, Issue 3, p. 262-270
Simultaneous saccharification and fermentation of cellulose: Effect of ethanol on enzymatic saccharification of cellulose
journal, April 1985
- Ooshima, Hiroshi; Ishitani, Yohsuke; Harano, Yoshio
- Biotechnology and Bioengineering, Vol. 27, Issue 4, p. 389-397
Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes
journal, April 2005
- Pajunen, M. I.
- Microbiology, Vol. 151, Issue 4, p. 1209-1218
Biotransformation of Toxic Organic and Inorganic Contaminants by Halophilic Bacteria
book, January 2004
- Peyton, Brent M.; Mormile, Melanie R.; Alva, Victor
- Halophilic Microorganisms, p. 315-331
The Path Forward for Biofuels and Biomaterials
journal, January 2006
- Ragauskas, Arthur J.; Williams, Charlotte K.; Davison, Brian H.
- Science, Vol. 311, Issue 5760, p. 484-489
Biomechanical and Biochemical Pulping of Sugarcane Bagasse with Ceriporiopsis subvermispora Fungal and Xylanase Pretreatments
journal, March 2001
- Ramos, Juan; González, Mercedes; RamÃrez, Felipe
- Journal of Agricultural and Food Chemistry, Vol. 49, Issue 3, p. 1180-1186
Quantitative Saccharification of Wood and Cellulose
journal, January 1945
- Saeman, Jerome F.; Bubl, Janet L.; Harris, Elwin E.
- Industrial & Engineering Chemistry Analytical Edition, Vol. 17, Issue 1, p. 35-37
Dilute Acid Pretreatment, Enzymatic Saccharification, and Fermentation of Rice Hulls to Ethanol
journal, January 2005
- Saha, Badal C.; Iten, Loren B.; Cotta, Michael A.
- Biotechnology Progress, Vol. 21, Issue 3, p. 816-822
Rapid production of thermostable cellulase-free xylanase by a strain of Bacillus subtilis and its properties
journal, June 2002
- Sá-Pereira, Paula; Mesquita, Alexandra; Duarte, José C.
- Enzyme and Microbial Technology, Vol. 30, Issue 7, p. 924-933
X-ray Structures of the Maltose–Maltodextrin-binding Protein of the Thermoacidophilic Bacterium Alicyclobacillus acidocaldarius Provide Insight into Acid Stability of Proteins
journal, January 2004
- Schäfer, Karsten; Magnusson, Ulrika; Scheffel, Frank
- Journal of Molecular Biology, Vol. 335, Issue 1, p. 261-274
Prokaryotic glycosylation
journal, February 2001
- Schäffer, Christina; Graninger, Michael; Messner, Paul
- PROTEOMICS, Vol. 1, Issue 2
Functional reconstitution of a maltose ATP-binding cassette transporter from the thermoacidophilic gram-positive bacterium Alicyclobacillus acidocaldarius
journal, May 2004
- Scheffel, Frank; Fleischer, Rebecca; Schneider, Erwin
- Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1656, Issue 1, p. 57-65
Dilute-Sulfuric Acid Pretreatment of Corn Stover in Pilot-Scale Reactor
book, January 2003
- Schell, Daniel J.; Farmer, Jody; Newman, Millie
- Biotechnology for Fuels and Chemicals
Purification, Properties and Structural Aspects of a Thermoacidophilic alpha-Amylase from Alicyclobacillus Acidocaldarius Atcc 27009
journal, December 1994
- Schwermann, Birgit; Pfau, Karsten; Liliensiek, Birgit
- European Journal of Biochemistry, Vol. 226, Issue 3, p. 981-991
Melamine Deaminase and Atrazine Chlorohydrolase: 98 Percent Identical but Functionally Different
journal, April 2001
- Seffernick, J. L.; de Souza, M. L.; Sadowsky, M. J.
- Journal of Bacteriology, Vol. 183, Issue 8, p. 2405-2410
Microbial hemicellulases
journal, June 2003
- Shallom, Dalia; Shoham, Yuval
- Current Opinion in Microbiology, Vol. 6, Issue 3, p. 219-228
An extremely thermostable xylanase from the thermophilic eubacterium Thermotoga
journal, July 1991
- Simpson, H D; Haufler, U R; Daniel, R M
- Biochemical Journal, Vol. 277, Issue 2, p. 413-417
Cellulase-free xylanases from Bacillus and other microorganisms
journal, February 2000
- Subramaniyan, S.; Prema, P.
- FEMS Microbiology Letters, Vol. 183, Issue 1, p. 1-7
Glycosyl hydrolases from hyperthermophiles
journal, February 1997
- Sunna, Anwar; Moracci, Marco; Rossi, Mose
- Extremophiles, Vol. 1, Issue 1, p. 2-13
Production of a cellulase-free xylanase from agricultural waste materials by a thermotolerant Streptomyces sp.
journal, October 2001
- Techapun, Charin; Sinsuwongwat, Suphawat; Poosaran, Naiyatat
- Biotechnology Letters, Vol. 23, Issue 20, p. 1685-1689
Comparison of pretreatment methods on the basis of available surface area
journal, January 1992
- Thompson, David N.; Chen, Hsin-Chih; Grethlein, Hans E.
- Bioresource Technology, Vol. 39, Issue 2, p. 155-163
In vitro degradation of natural insoluble lignin in aqueous media by the extracellular peroxidases of Phanerochaete chrysosporium
journal, March 1998
- Thompson, David N.; Hames, Bonnie R.; Reddy, C. Adinarayana
- Biotechnology and Bioengineering, Vol. 57, Issue 6, p. 704-717
Measurement of fumonisins in corn with a fiber optic fluoroimmunosensor
conference, May 1997
- Thompson, Vicki S.; Maragos, Chris M.; Thompson, Richard B.
- Advances in Fluorescence Sensing Technology III, Vol. 2980
Preliminary Investigation of Fungal Bioprocessing of Wheat Straw for Production of Straw-Thermoplastic Composites
book, January 2003
- Thompson, David N.; Houghton, Tracy P.; Lacey, Jeffrey A.
- Biotechnology for Fuels and Chemicals, p. 423-436
Purification and Characterization of a Novel Thermo-Alkali-Stable Catalase from Thermus brockianus
journal, January 2003
- Thompson, Vicki S.; Schaller, Kastli D.; Apel, William A.
- Biotechnology Progress, Vol. 19, Issue 4, p. 1292-1299
Potential and utilization of thermophiles and thermostable enzymes in biorefining
journal, January 2007
- Turner, Pernilla; Mamo, Gashaw; Karlsson, Eva
- Microbial Cell Factories, Vol. 6, Issue 1, 9
The first description of an archaeal hemicellulase: the xylanase from Thermococcus zilligii strain AN1
journal, November 1999
- Uhl, Andreas M.; Daniel, Roy M.
- Extremophiles, Vol. 3, Issue 4, p. 263-267
Bacterial glycoproteins: Functions, biosynthesis and applications
journal, April 2003
- Upreti, Raj K.; Kumar, Manoj; Shankar, V.
- PROTEOMICS, Vol. 3, Issue 4, p. 363-379
Xylanases in bleaching: From an idea to the industry
journal, March 1994
- Viikari, Liisa; Kantelinen, Anne; Sundquist, Jorma
- FEMS Microbiology Reviews, Vol. 13, Issue 2-3, p. 335-350
Strand displacement amplification—an isothermal, in vitro DNA amplification technique
journal, January 1992
- Walker, G. Terrance; Fraiser, Melinda S.; Schram, James L.
- Nucleic Acids Research, Vol. 20, Issue 7, p. 1691-1696
Characterization of a new bacteriophage which infects bacteria of the genus Acidiphilium
journal, November 1993
- Ward, T. E.; Bruhn, D. F.; Shean, M. L.
- Journal of General Virology, Vol. 74, Issue 11, p. 2419-2425
Electrotransformation of Acidophilic, Heterotrophic, Gram-negative Bacteria
book, January 2000
- Ward, Thomas E.; Eynard, Natalie; Teissié, Justin
- Electrotransformation of Bacteria, p. 94-103
Conversion of a β-Ketoacyl Synthase to a Malonyl Decarboxylase by Replacement of the Active-Site Cysteine with Glutamine
journal, September 1999
- Witkowski, Andrzej; Joshi, Anil K.; Lindqvist, Ylva
- Biochemistry, Vol. 38, Issue 36, p. 11643-11650
Process for pretreating cellulosic substrates and for producing sugar therefrom
patent, December 1980
- Grethlein, Hans E.
- US Patent Document 4,237,226
Method of detecting and characterizing a nucleic acid or reactant for the application of this method
patent, April 1986
- Kourilsky, Philippe; Avrameas, Stratis; Cami nee Contamine, Brigitte
- US Patent Document 4,581,333
Plasmid, method for construction of the same, microorganism carrying the plasmid and method for cultivation of the microorganism
patent, November 1986
- Horikoshi, Koki; Kudo, Toshiaki; Honda, Hiroshi
- US Patent Document 4,624,922
Probe containing a modified nucleic acid recognizable by specific antibodies and use of this probe to detect and characterize a homologous dna sequence
patent, March 1992
- Tchen, Paul; Kourilsky, Philip; Leng, Marc
- US Patent Document 5,098,825
Thermostable .alpha.-L-arabinofuranosidase from Aureobasidium pullulans
patent, March 1999
- Saha, Badal C.; Bothast, Rodney J.
- US Patent Document 5,882,905
Thermostable xylanase
patent, June 1999
- Fukunaga, Nobuyuki; Iwasaki, Yuji; Kono, Satoko
- US Patent Document 5,916,795
Xylanase obtained from an anaerobic fungus
patent, September 1999
- Cheng, Kuo-Joan; Selinger, Leonard Brent; Liu, Jin-Hao
- US Patent Document 5,948,667
Thermostable xylanases
patent, July 2000
- Gronberg, Vidar; Forster, Simon; Moody, Dean
- US Patent Document 6,083,733
Xyloglucan-specific alkaline xyloglucanase from bacillus
patent, July 2001
- Schulein, Martin; Outtrup, Helle; Jorgensen, Per Lina
- US Patent Document 6,268,197
Xylanase derived from a Bacillus species, expression vectors for such xylanase and other proteins, host organisms therefor and use thereof
patent, July 2002
- De Buyl, Eric; Lahaye, Andrée; Ledoux, Pierre
- US Patent Document 6,426,211
Polypeptides having haloperoxidase activity
patent, January 2003
- Danielsen, Steffen; Schneider, Palle
- US Patent Document 6,506,585
Microbial process for producing L-ascorbic acid, D-erythorbic acid, and salts thereof
patent, August 2004
- Asakura, Akira; Hoshino, Tatsuo; Shinjoh, Masako
- US Patent Document 6,777,212
‘Pseudomonas stutzeri’ strain and process for preparation of xylanase
patent, December 2004
- Bhosle, Narayan Baburao; Giriyan, Asha
- US Patent Document 6,833,259
Enzyme and methodology for the treatment of a biomass
patent, June 2010
- Thompson, Vicki S.; Thompson, David N.; Schaller, Kastli D.
- US Patent Document 7,727,755
Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
patent, December 2010
- Thompson, David N.; Apel, William A.; Thompson, Vicki S.
- US Patent Document 7,858,353
Thermal and acid tolerant beta-xylosidases, genes encoding, related organisms, and methods
patent, April 2011
- Thompson, David N.; Thompson, Vicki S.; Schaller, Kastli D.
- US Patent Document 7,923,234
Thermophilic and thermoacidophilic sugar transporter genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
patent, June 2011
- Thompson, David N.; Apel, William A.; Thompson, Vicki S.
- US Patent Document 7,960,534
Thermophilic and thermoacidophilic sugar transporter genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
patent, December 2011
- Thompson, David N.; Apel, William A.; Thompson, Vicki S.
- US Patent Document 8,071,748
Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
patent, June 2012
- Thompson, David N.; Apel, William A.; Thompson, Vicki S.
- US Patent Document 8,202,716
Thermal and acid tolerant beta xylosidases, arabinofuranosidases, genes encoding, related organisms, and methods
patent, April 2013
- Thompson, David N.; Thompson, Vicki S.; Schaller, Kastli D.
- US Patent Document 8,431,379
Process for hydrolyzing starch without pH adjustment
patent-application, July 2003
- Shetty, Jayarama K.; Singley, Eric C.; Strohm, Bruce A.
- US Patent Application 10/026288; 20030134395
Tomato plants that exhibit resistance to botrytis cinerea
patent-application, December 2003
- Gabor, Brad; Frampton, Anna; Bragaloni, Mauro
- US Patent Application 10/278360; 20030233674
Expression of microbial proteins in plants for production of plants with improved properties
patent-application, December 2003
- Cao, Yongwei; Hinkle, Gregory J.; Slater, Steven C.
- US Patent Application 10/369493; 20030233675
Identification of essential genes in microorganisms
patent-application, February 2004
- Wang, Liangsu; Zamudio, Carlos; Malone, Cheryl
- US Patent Application 10/282122; 20040029129
High temperature and alkaline stable catalase
patent-application, May 2005
- Thompson, Vicki; Apel, William A.; Schaller, Kastli D.
- US Patent Application 10/981434; 20050112742
Promoters and proteins from Clostridium thermocellum and uses thereof
patent-application, May 2006
- Wu, J.H. David; Newcomb, Michael
- US Patent Application 11/271287; 20060105442
Products and processes for in vitro synthesis of biomolecules
patent-application, September 2006
- Katzen, Federico; Kudlicki, Wieslaw; Fletcher, Julia
- US Patent Application 11/336644; 20060211083
Polypeptides of Alicyclobacillus sp.
patent-application, April 2007
- Wilting, Reinhard; Lassen, Soren Flensted; Ostergaard, Peter Rahbek
- US Patent Application 11/636950; 20070082381
Enzyme and methodology for the treatment of a biomass
patent-application, May 2007
- Thompson, Vicki S.; Thompson, David N.; Schaller, Kastli D.
- US Patent Application; 11/266063; 20070099282
Compositions and methods for the synthesis and subsequent modification of uridine-5'-diphosphosulfoquinovose (UDP-SQ)
patent-application, June 2007
- Benning, Christoph; Sanda, Sherrie Lea; Yu, Bin
- US Patent Application 11/590541; 20070134778
Synthesis of oligosaccharides, glycolipds, and glycoproteins using bacterial glycosyltransferases
patent-application, June 2007
- Johnson, Karl F.; Bezila, Daniel James; Taylor, Diane E.
- US Patent Application PCT/US03/23155; 20070148728
Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
patent-application, August 2009
- Thompson, David N.; Apel, William A.; Thompson, Vicki S.
- US Patent Apllication 12/322359; 20090203107
Transcriptional control in alicyclobacillus acidocaldarius and associated genes, proteins, and methods
patent-application, August 2009
- Lee, Brady D.; Thompson, David N.; Apel, William A.
- US Patent Application 12/380008; 20090215168
Thermophilic Organisms For Conversion Of Lignocellulosic Biomass To Ethanol
patent-application, September 2009
- Shaw, IV, Arthur; Desai, Sunil G.; Lynd, Lee R.
- US Patent Application PCT/US07/67941; 20090221049
Thermal and acid tolerant beta-xylosidases, genes encoding, related organisms, and methods
patent-application, September 2009
- Thompson, David N.; Thompson, Vicki S.; Schaller, Kastli D.
- US Patent Application 12/321636; 20090226978
Thermophilic and thermoacidophilic sugar transporter genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
patent-application, October 2009
- Thompson, David N.; Apel, William A.; Thompson, Vicki S.
- US Patent Application 12/380554; 20090253205
Thermophilic and thermoacidophilic glycosylation genes and enzymes from Alicyclobacillus acidocaldarius and related organisms, methods
patent-application, October 2009
- Thompson, David N.; Apel, William A.; Thompson, Vicki S.
- US Patent Application 12/380450; 20090263859
Thermophilic and thermoacidophilic metabolism genes and enzymes from alicyclobacillus acidocaldadarius and related organisms, methods
patent-application, October 2009
- Thompson, Vicki S.; Apel, William A.; Reed, David W
- US Patent Application 12/380551; 20090269827
Alteration and modulation of protein activity by varying post-translational modification
patent-application, August 2010
- Thompson, David N.; Reed, David W.; Thompson, Vicki S.
- US Patent Application 12/655993; 20100203583
Thermal and acid tolerant beta xylosidases, arabinofuranosidases, genes encoding, related organisms, and methods
patent-application, December 2010
- Thompson, David N.; Thompson, Vicki S.; Schaller, Kastli D.
- US Patent Application 12/802911; 20100311110
Methods of combined bioprocessing and related microorganisms, thermophilic and/or acidophilic enzymes, and nucleic acids encoding said enzymes
patent-application, April 2011
- Thompson, David; Apel, William; Thompson, Vicki
- US Patent Application 12/587229; 20110081683
Genetic elements, proteins, and associated methods including application of additional genetic information to gram (+) thermoacidophiles
patent-application, November 2011
- Lee, Brady; Newby, Deborah; Lacey, Jeffrey
- US Patent Application 12/800045; 20110275135
Thermophilic and thermoacidophilic biopolymer-degrading genes and enzymes from alicyclobacillus acidocaldarius and related organisms, methods
patent-application, January 2012
- Thompson, David N.; Apel, William A.; Thompson, Vicki S.
- US Patent Application 12/927490; 20120015407