In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris
Abstract
The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris. Methods for producing polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris are also provided.
- Inventors:
- Issue Date:
- Research Org.:
- The Scripps Research Inst., La Jolla, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1375232
- Patent Number(s):
- 9732349
- Application Number:
- 14/165,199
- Assignee:
- The Scripps Research Institute
- Patent Classifications (CPCs):
-
C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG03-00ER46051
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2014 Jan 27
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Young, Travis, and Schultz, Peter G. In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris. United States: N. p., 2017.
Web.
Young, Travis, & Schultz, Peter G. In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris. United States.
Young, Travis, and Schultz, Peter G. Tue .
"In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris". United States. https://www.osti.gov/servlets/purl/1375232.
@article{osti_1375232,
title = {In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris},
author = {Young, Travis and Schultz, Peter G.},
abstractNote = {The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris. Methods for producing polypeptides comprising unnatural amino acids in methylotrophic yeast such as Pichia pastoris are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 15 00:00:00 EDT 2017},
month = {Tue Aug 15 00:00:00 EDT 2017}
}
Works referenced in this record:
Glycoprotein synthesis
patent, August 2005
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 6,927,042
In vivo incorporation of unnatural amino acids
patent, May 2006
- Schultz, Peter G.; Wang, Lei; Anderson, John Christopher
- US Patent Document 7,045,337
Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
patent, August 2006
- Schultz, Peter G.; Wang, Lei; Anderson, John Christopher
- US Patent Document 7,083,970
Glycoprotein synthesis
patent, October 2006
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,129,333
Glycoprotein synthesis
patent, February 2007
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,183,082
Glycoprotein synthesis
patent, April 2007
- Shultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,199,222
Glycoprotein synthesis
patent, May 2007
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,217,809
Glycoprotein synthesis
patent, July 2007
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,238,510
Glycoprotein synthesis
patent, August 2007
- Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
- US Patent Document 7,262,040
Derivatization of non-natural amino acids and polypeptides
patent, June 2008
- Miao, Zhenwei; Liu, Junjie; Norman, Thea
- US Patent Document 7,385,028
Binding polypeptides and uses thereof
patent, February 2015
- Sidhu, Sachdev; Birtalan, Sara C.; Fellouse, Frederic
- US Patent Document 8,957,187
patent-application, October 2004
- Schultz, Peter G.; Wang, Lei
- US Patent Application 10/744899; 20040198637
Unnatural reactive amino acid genetic code additions
patent-application, December 2004
- Deiters, Alexander; Cropp, T. Ashton; Chin, Jason W.
- US Patent Application 10/826919; 20040265952
Expanding the eukaryotic genetic code
patent-application, January 2005
- Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
- US Patent Application 10/825867; 20050009049
Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, June 2005
- Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter
- US Patent Application 11/016348; 20050136513
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, September 2005
- Schultz, Peter; Wang, Lei; Anderson, John Christopher
- US Patent Application 11/009635; 20050208536
Site-specific incorporation of redox active amino acids into proteins
patent-application, October 2005
- Alfonta, Lital; Schultz, Peter G.; Zhang, Zhiwen
- US Patent Application 10/965218; 20050227318
In vivo incorporation of unnatural amino acids
patent-application, November 2005
- Schultz, Peter; Wang, Lei; Anderson, John Christopher
- US Patent Application 11/017550; 20050250183
Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
patent-application, December 2005
- Xie, Jianming; Wang, Lei; Wu, Ning
- US Patent Application 11/137850; 20050272121
Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, March 2006
- Schultz, Peter; Wang, Lei; Anderson, John Christopher
- US Patent Application 11/002387; 20060063244
In vivo incorporation of alkynyl amino acids into proteins in eubacteria
patent-application, April 2006
- Deiters, Alexander; Schultz, Peter
- US Patent Application 11/232425; 20060073507
Site-specific labeling of proteins for NMR studies
patent-application, May 2006
- Deiters, Alexander; Geierstanger, Bernhard H.; Schultz, Peter G.
- US Patent Application 11/233466; 20060110784
In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria
patent-application, May 2006
- Schultz, Peter G.; Hanson, Sarah R.; Xu, Ran
- US Patent Application 11/255601; 20060110796
Adding photoregulated amino acids to the genetic code
patent-application, June 2006
- Deiters, Alexander; Wu, Ning; Schultz, Peter G.
- US Patent Application 11/233508; 20060134746
Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, July 2006
- Anderson, J. Christopher; Schultz, Peter G.
- US Patent Application 10/563655; 20060160175
Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
patent-application, August 2006
- Anderson, J. Christopher; Wu, Ning; Santoro, Stephen
- US Patent Application 11/326970; 20060177900
Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
patent-application, September 2006
- Miao, Zhenwei; Liu, Junjie; Norman, Thea
- US Patent Application 11/313956; 20060217532
Unnatural reactive amino acid genetic code additions
patent-application, November 2006
- Deiters, Alexander; Cropp, T. Ashton; Chin, Jason W.
- US Patent Application 10/561121; 20060246509
Site-specific incorporation of redox active amino acids into proteins
patent-application, January 2007
- Alfonta, Lital; Schultz, Peter G.; Zhang, Zhiwen
- US Patent Application 10/575991; 20070009990
Composition of orthogonal glutamyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, January 2007
- Anderson, J. Christopher; Schultz, Peter G.; Santoro, Stephen
- US Patent Application 10/563656; 20070020634
Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, February 2007
- Anderson, J. Christopher; Wu, Ning; Santoro, Stephen
- US Patent Application 10/563686; 20070042461
Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, May 2007
- Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter
- US Patent Application 10/580987; 20070111193
In vivo incorporation of unnatural amino acids
patent-application, May 2007
- Schultz, Peter; Wang, Lei; Anderson, John Christopher
- US Patent Application 11/583551; 20070117184
Expanding the eukaryotic genetic code
patent-application, July 2007
- Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
- US Patent Application 11/710655; 20070154952
Binding Polypeptides With Diversified And Consensus VH/VL Hypervariable Sequences
patent-application, July 2007
- Dennis, Mark; Liang, Wei-Ching; Wu, Yan
- US Patent Application 11/557466; 20070160598
Expanding the eukaryotic genetic code
patent-application, July 2007
- Chin, Jason; Cropp, T. Ashton; Anderson, J. Christopher
- US Patent Application 11/710672; 20070166791
Glycoprotein synthesis
patent-application, July 2007
- Schultz, Peter; Wang, Lei; Zhang, Zhiwen
- US Patent Application 11/580203; 20070172915
Selective posttranslational modification of phage-displayed polypeptides
patent-application, August 2007
- Tsao, Meng-Lin; Tian, Feng; Schultz, Peter
- US Patent Application 11/580223; 20070178448
Glycoprotein synthesis
patent-application, August 2007
- Schultz, Peter; Wang, Lei; Zhang, Zhiwen
- US Patent Application 11/520849; 20070184517
Binding Polypeptides and Uses Thereof
patent-application, August 2007
- Sidhu, Sachdev; Birtalan, Sara; Fellouse, Frederic
- US Patent Application 11/566120; 20070202552
Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
patent-application, October 2007
- Wang, Jiangyun; Schultz, Peter
- US Patent Application 11/724039; 20070238152
Systems for the expression of orthogonal translation components in eubacterial host cells
patent-application, December 2007
- Ryu, Youngha; Schultz, Peter
- US Patent Application 11/715672; 20070281335
Modified Human Growth Hormone
patent-application, May 2008
- Cho, Ho Sung; Daniel, Thomas; Dimarchi, Richard
- US Patent Application 11/923992; 20080102124
Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
patent-application, November 2010
- Young, Travis; Schultz, Peter G.
- US Patent Application 12/653274; 20100297693
Genomic Exploration of the Hemiascomycetous Yeasts: 15. Pichia sorbitophila
journal, December 2000
- de Montigny, Jacky; Spehner, Catherine; Souciet, Jean-Luc
- FEBS Letters, Vol. 487, Issue 1, p. 87-90
High-Level Expression of Tetanus Toxin Fragment C in Pichia Pastoris Strains Containing Multiple Tandem Integrations of the Gene
journal, May 1991
- Clare, J. J.; Rayment, F. B.; Ballantine, S. P.
- Nature Biotechnology, Vol. 9, Issue 5, p. 455-460
Yeast Systems for the Commercial Production of Heterologous Proteins
journal, November 1991
- Buckholz, Richard G.; Gleeson, Martin A. G.
- Bio/Technology, Vol. 9, Issue 11, p. 1067-1072
Heterologous protein expression in the methylotrophic yeast Pichia pastoris
journal, January 2000
- Cereghino, Joan Lin; Cregg, James M.
- FEMS Microbiology Reviews, Vol. 24, Issue 1, p. 45-66
An Improved System for the Generation and Analysis of Mutant Proteins Containing Unnatural Amino Acids in Saccharomyces cerevisiae
journal, August 2007
- Chen, Shawn; Schultz, Peter G.; Brock, Ansgar
- Journal of Molecular Biology, Vol. 371, Issue 1, p. 112-122
Addition of p-Azido-L -phenylalanine to the Genetic Code of Escherichia coli
journal, July 2002
- Chin, Jason W.; Santoro, Stephen W.; Martin, Andrew B.
- Journal of the American Chemical Society, Vol. 124, Issue 31, p. 9026-9027
An Expanded Eukaryotic Genetic Code
journal, August 2003
- Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
- Science, Vol. 301, Issue 5635, p. 964-967
Expression of heterologous proteins in Pichia pastoris: a useful experimental tool in protein engineering and production
journal, January 2005
- Daly, Rachel; Hearn, Milton T. W.
- Journal of Molecular Recognition, Vol. 18, Issue 2, p. 119-138
Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
journal, October 2003
- Deiters, Alexander; Cropp, T. Ashton; Mukherji, Mridul
- Journal of the American Chemical Society, Vol. 125, Issue 39, p. 11782-11783
Nucleophilic Catalysis of Oxime Ligation
journal, November 2006
- Dirksen, Anouk; Hackeng, Tilman M.; Dawson, Philip E.
- Angewandte Chemie International Edition, Vol. 45, Issue 45, p. 7581-7584
tRNA genes and retroelements in the yeast genome
journal, February 1998
- Hani, J.
- Nucleic Acids Research, Vol. 26, Issue 3
Overview of Protein Expression in Pichia pastoris
book, December 1995
- Higgins, David R.; Coligan, John E.; Dunn, Ben M.
- Current Protocols in Protein Science
Development and Characterization of a Glucagon-Like Peptide 1-Albumin Conjugate: The Ability to Activate the Glucagon-Like Peptide 1 Receptor In Vivo
journal, March 2003
- Kim, J. -G.; Baggio, L. L.; Bridon, D. P.
- Diabetes, Vol. 52, Issue 3
Albumin as a drug carrier: Design of prodrugs, drug conjugates and nanoparticles
journal, December 2008
- Kratz, Felix
- Journal of Controlled Release, Vol. 132, Issue 3, p. 171-183
Control of protein phosphorylation with a genetically encoded photocaged amino acid
journal, October 2007
- Lemke, Edward A.; Summerer, Daniel; Geierstanger, Bernhard H.
- Nature Chemical Biology, Vol. 3, Issue 12, p. 769-772
Optimization of humanized IgGs in glycoengineered Pichia pastoris
journal, January 2006
- Li, Huijuan; Sethuraman, Natarajan; Stadheim, Terrance A.
- Nature Biotechnology, Vol. 24, Issue 2, p. 210-215
Detailed Analysis Concerning the Biodistribution and Metabolism of Human Calcitonin-Derived Cell-Penetrating Peptides
journal, August 2008
- Neundorf, I.; Rennert, R.; Franke, J.
- Bioconjugate Chemistry, Vol. 19, Issue 8, p. 1596-1603
Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation
journal, January 2005
- Ohya, Tomoshi; Ohyama, Masao; Kobayashi, Kaoru
- Biotechnology and Bioengineering, Vol. 90, Issue 7, p. 876-887
A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code
journal, September 2000
- Pastrnak, Miro; Magliery, Thomas J.; Schultz, Peter G.
- Helvetica Chimica Acta, Vol. 83, Issue 9, p. 2277-2286
A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris
journal, August 1998
- Shen, Shigang; Sulter, Greitje; Jeffries, Thomas W.
- Gene, Vol. 216, Issue 1, p. 93-102
High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris
journal, May 1989
- Sreekrishna, K.; Nelles, Lynn; Potenz, Rica
- Biochemistry, Vol. 28, Issue 9
Unnatural Amino Acid Incorporation into Virus-Like Particles
journal, April 2008
- Strable, Erica; Prasuhn, Duane E.; Udit, Andrew K.
- Bioconjugate Chemistry, Vol. 19, Issue 4, p. 866-875
A genetically encoded fluorescent amino acid
journal, June 2006
- Summerer, D.; Chen, S.; Wu, N.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 26, p. 9785-9789
PEGylation, successful approach to drug delivery
journal, November 2005
- Veronese, Francesco M.; Pasut, Gianfranco
- Drug Discovery Today, Vol. 10, Issue 21, p. 1451-1458
New Methods Enabling Efficient Incorporation of Unnatural Amino Acids in Yeast
journal, May 2008
- Wang, Qian; Wang, Lei
- Journal of the American Chemical Society, Vol. 130, Issue 19, p. 6066-6067
Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002
- Wang, L.; Zhang, Z.; Brock, A.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 1, p. 56-61
Adding amino acids to the genetic repertoire
journal, December 2005
- Xie, Jianming; Schultz, Peter G.
- Current Opinion in Chemical Biology, Vol. 9, Issue 6, p. 548-554
Expanding the Genetic Repertoire of the Methylotrophic Yeast Pichia pastoris
journal, March 2009
- Young, Travis S.; Ahmad, Insha; Brock, Ansgar
- Biochemistry, Vol. 48, Issue 12, p. 2643-2653
Developing high cell density fed-batch cultivation strategies for heterologous protein production in Pichia pastoris using the nitrogen source-regulated FLD1 Promoter
journal, May 2005
- Resina, David; Cos, Oriol; Ferrer, Pau
- Biotechnology and Bioengineering, Vol. 91, Issue 6, p. 760-767