Title: In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris

Abstract

The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methyltrophic yeast such as Pichia pastoris. Methods for producing polypeptides comprising unnatural amino acids in methyltrophic yeast such as Pichia pastoris are also provided.

Inventors:

Young, Travis ^[1]; Schultz, Peter G ^[2]

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+ Show Inventor Affiliations

1. San Diego, CA
2. La Jolla, CA

Issue Date:

2014-02-11

Research Org.:

The Scripps Research Institute, La Jolla, CA, USA

Sponsoring Org.:

USDOE

OSTI Identifier:

1119694

Patent Number(s):

8647840

Application Number:

12/734,995

Assignee:

The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

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C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K2319/00 - Fusion polypeptide

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/815 - {for yeasts other than Saccharomyces}
C12N9/93 - {Ligases

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C07K2319/55 - containing a fusion with a toxin, e.g. diphteria toxin

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

C12P21/02 - having a known sequence of two or more amino acids, e.g. glutathione

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DOE Contract Number:  

FG03-00ER46051

Resource Type:

Patent

Country of Publication:

United States

Language:

English

Subject:

59 BASIC BIOLOGICAL SCIENCES