In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris

Young, Travis; Schultz, Peter G

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Title: In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris

Abstract

The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methyltrophic yeast such as Pichia pastoris. Methods for producing polypeptides comprising unnatural amino acids in methyltrophic yeast such as Pichia pastoris are also provided.

Inventors:

Young, Travis ^[1]; Schultz, Peter G ^[2]

San Diego, CA
La Jolla, CA

Issue Date:: 2014-02-11

Research Org.:: The Scripps Research Institute, La Jolla, CA, USA

Sponsoring Org.:: USDOE

OSTI Identifier:: 1119694

Patent Number(s):: 8647840

Application Number:: 12/734,995

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

Show more

C - CHEMISTRY C07 - ORGANIC CHEMISTRY C07K - PEPTIDES
C07K2319/00 - Fusion polypeptide
C07K2319/55 - containing a fusion with a toxin, e.g. diphteria toxin

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/815 - {for yeasts other than Saccharomyces}
C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P21/02 - having a known sequence of two or more amino acids, e.g. glutathione

Show less

DOE Contract Number:: FG03-00ER46051

Resource Type:: Patent

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Young, Travis, and Schultz, Peter G. In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris.  United States: N. p., 2014. 
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                    Young, Travis, & Schultz, Peter G. In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris.  United States.

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                    Young, Travis, and Schultz, Peter G. Tue .  
        "In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris".  United States.  https://www.osti.gov/servlets/purl/1119694.

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@article{osti_1119694,

  title        = {In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris},

  author       = {Young, Travis and Schultz, Peter G},

  abstractNote = {The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methyltrophic yeast such as Pichia pastoris. Methods for producing polypeptides comprising unnatural amino acids in methyltrophic yeast such as Pichia pastoris are also provided.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2014},

  month        = {2}

}

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Works referenced in this record:

Glycoprotein synthesis
patent, August 2005

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 6,927,042
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/6927042

In vivo incorporation of unnatural amino acids
patent, May 2006

Schultz, Peter G.; Wang, Lei; Anderson, John Christopher
US Patent Document 7,045,337
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7045337

Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
patent, August 2006

Schultz, Peter G.; Wang, Lei; Anderson, John Christopher
US Patent Document 7,083,970
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7083970

Glycoprotein synthesis
patent, October 2006

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,129,333
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7129333

Glycoprotein synthesis
patent, February 2007

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,183,082
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7183082

Glycoprotein synthesis
patent, April 2007

Shultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,199,222
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7199222

Glycoprotein synthesis
patent, May 2007

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,217,809
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7217809

Glycoprotein synthesis
patent, July 2007

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,238,510
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7238510

Glycoprotein synthesis
patent, August 2007

Schultz, Peter G.; Wang, Lei; Zhang, Zhiwen
US Patent Document 7,262,040
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7262040

Derivatization of non-natural amino acids and polypeptides
patent, June 2008

Miao, Zhenwei; Liu, Junjie; Norman, Thea
US Patent Document 7,385,028
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/7385028

patent-application, October 2004

Schultz, Peter G.; Wang, Lei
US Patent Application 10/744899; 20040198637
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040198637

Unnatural reactive amino acid genetic code additions
patent-application, December 2004

Deiters, Alexander; Cropp, T. Ashton; Chin, Jason W.
US Patent Application 10/826919; 20040265952
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20040265952

Expanding the eukaryotic genetic code
patent-application, January 2005

Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
US Patent Application 10/825867; 20050009049
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050009049

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, June 2005

Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter
US Patent Application 11/016348; 20050136513
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050136513

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, September 2005

Schultz, Peter; Wang, Lei; Anderson, John Christopher
US Patent Application 11/009635; 20050208536
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050208536

Site-specific incorporation of redox active amino acids into proteins
patent-application, October 2005

Alfonta, Lital; Schultz, Peter G.; Zhang, Zhiwen
US Patent Application 10/965218; 20050227318
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050227318

In vivo incorporation of unnatural amino acids
patent-application, November 2005

Schultz, Peter; Wang, Lei; Anderson, John Christopher
US Patent Application 11/017550; 20050250183
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050250183

Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
patent-application, December 2005

Xie, Jianming; Wang, Lei; Wu, Ning
US Patent Application 11/137850; 20050272121
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20050272121

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, March 2006

Schultz, Peter; Wang, Lei; Anderson, John Christopher
US Patent Application 11/002387; 20060063244
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060063244

In vivo incorporation of alkynyl amino acids into proteins in eubacteria
patent-application, April 2006

Deiters, Alexander; Schultz, Peter
US Patent Application 11/232425; 20060073507
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060073507

Site-specific labeling of proteins for NMR studies
patent-application, May 2006

Deiters, Alexander; Geierstanger, Bernhard H.; Schultz, Peter G.
US Patent Application 11/233466; 20060110784
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060110784

In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria
patent-application, May 2006

Schultz, Peter G.; Hanson, Sarah R.; Xu, Ran
US Patent Application 11/255601; 20060110796
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060110796

Adding photoregulated amino acids to the genetic code
patent-application, June 2006

Deiters, Alexander; Wu, Ning; Schultz, Peter G.
US Patent Application 11/233508; 20060134746
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060134746

Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, July 2006

Anderson, J. Christopher; Schultz, Peter G.
US Patent Application 10/563655; 20060160175
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060160175

Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
patent-application, August 2006

Anderson, J. Christopher; Wu, Ning; Santoro, Stephen
US Patent Application 11/326970; 20060177900
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060177900

Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
patent-application, September 2006

Miao, Zhenwei; Liu, Junjie; Norman, Thea
US Patent Application 11/313956; 20060217532
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060217532

Unnatural reactive amino acid genetic code additions
patent-application, November 2006

Deiters, Alexander; Cropp, T. Ashton; Chin, Jason W.
US Patent Application 10/561121; 20060246509
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20060246509

Site-specific incorporation of redox active amino acids into proteins
patent-application, January 2007

Alfonta, Lital; Schultz, Peter G.; Zhang, Zhiwen
US Patent Application 10/575991; 20070009990
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070009990

Composition of orthogonal glutamyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, January 2007

Anderson, J. Christopher; Schultz, Peter G.; Santoro, Stephen
US Patent Application 10/563656; 20070020634
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070020634

Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, February 2007

Anderson, J. Christopher; Wu, Ning; Santoro, Stephen
US Patent Application 10/563686; 20070042461
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070042461

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, May 2007

Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter
US Patent Application 10/580987; 20070111193
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070111193

In vivo incorporation of unnatural amino acids
patent-application, May 2007

Schultz, Peter; Wang, Lei; Anderson, John Christopher
US Patent Application 11/583551; 20070117184
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070117184

Expanding the eukaryotic genetic code
patent-application, July 2007

Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
US Patent Application 11/710655; 20070154952
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070154952

Binding Polypeptides With Diversified And Consensus VH/VL Hypervariable Sequences
patent-application, July 2007

Dennis, Mark; Liang, Wei-Ching; Wu, Yan
US Patent Application 11/557466; 20070160598
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070160598

Expanding the eukaryotic genetic code
patent-application, July 2007

Chin, Jason; Cropp, T. Ashton; Anderson, J. Christopher
US Patent Application 11/710672; 20070166791
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070166791

Glycoprotein synthesis
patent-application, July 2007

Schultz, Peter; Wang, Lei; Zhang, Zhiwen
US Patent Application 11/580203; 20070172915
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070172915

Selective posttranslational modification of phage-displayed polypeptides
patent-application, August 2007

Tsao, Meng-Lin; Tian, Feng; Schultz, Peter
US Patent Application 11/580223; 20070178448
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070178448

Glycoprotein synthesis
patent-application, August 2007

Schultz, Peter; Wang, Lei; Zhang, Zhiwen
US Patent Application 11/520849; 20070184517
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070184517

Binding Polypeptides and Uses Thereof
patent-application, August 2007

Sidhu, Sachdev; Birtalan, Sara; Fellouse, Frederic
US Patent Application 11/566120; 20070202552
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070202552

Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
patent-application, October 2007

Wang, Jiangyun; Schultz, Peter
US Patent Application 11/724039; 20070238152
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070238152

Systems for the expression of orthogonal translation components in eubacterial host cells
patent-application, December 2007

Ryu, Youngha; Schultz, Peter
US Patent Application 11/715672; 20070281335
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070281335

Modified Human Growth Hormone
patent-application, May 2008

Cho, Ho Sung; Daniel, Thomas; Dimarchi, Richard
US Patent Application 11/923992; 20080102124
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20080102124

Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
patent-application, November 2010

Young, Travis; Schultz, Peter G.
US Patent Application 12/653274; 20100297693
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20100297693

Detailed Analysis Concerning the Biodistribution and Metabolism of Human Calcitonin-Derived Cell-Penetrating Peptides
journal, August 2008

Neundorf, I.; Rennert, R.; Franke, J.
Bioconjugate Chemistry, Vol. 19, Issue 8, p. 1596-1603
https://doi.org/10.1021/bc800149f

Yeast Systems for the Commercial Production of Heterologous Proteins
journal, November 1991

Buckholz, Richard G.; Gleeson, Martin A. G.
Bio/Technology, Vol. 9, Issue 11, p. 1067-1072
https://doi.org/10.1038/nbt1191-1067

Heterologous protein expression in the methylotrophic yeast Pichia pastoris
journal, January 2000

Cereghino, Joan Lin; Cregg, James M.
FEMS Microbiology Reviews, Vol. 24, Issue 1, p. 45-66
https://doi.org/10.1111/j.1574-6976.2000.tb00532.x

Expression of Foreign Genes in the yeast Pichia pastoris
book, January 2001

Cereghino, Geoffrey P. Lin; Sunga, Anthony J.; Cereghino, Joan Lin
Genetic Engineering: Principles and Methods
https://doi.org/10.1007/0-306-47572-3_9

An Improved System for the Generation and Analysis of Mutant Proteins Containing Unnatural Amino Acids in Saccharomyces cerevisiae
journal, August 2007

Chen, Shawn; Schultz, Peter G.; Brock, Ansgar
Journal of Molecular Biology, Vol. 371, Issue 1, p. 112-122
https://doi.org/10.1016/j.jmb.2007.05.017

An Expanded Eukaryotic Genetic Code
journal, August 2003

Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
Science, Vol. 301, Issue 5635, p. 964-967
https://doi.org/10.1126/science.1084772

Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review
journal, April 2006

Cos, Oriol; Ramón, Ramón; Montesinos, José Luis
Microbial Cell Factories, Vol. 5, Issue 1
https://doi.org/10.1186/1475-2859-5-17

Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
journal, October 2003

Deiters, Alexander; Cropp, T. Ashton; Mukherji, Mridul
Journal of the American Chemical Society, Vol. 125, Issue 39, p. 11782-11783
https://doi.org/10.1021/ja0370037

Nucleophilic Catalysis of Oxime Ligation
journal, November 2006

Dirksen, Anouk; Hackeng, Tilman M.; Dawson, Philip E.
Angewandte Chemie International Edition, Vol. 45, Issue 45, p. 7581-7584
https://doi.org/10.1002/anie.200602877

Control of protein phosphorylation with a genetically encoded photocaged amino acid
journal, October 2007

Lemke, Edward A.; Summerer, Daniel; Geierstanger, Bernhard H.
Nature Chemical Biology, Vol. 3, Issue 12, p. 769-772
https://doi.org/10.1038/nchembio.2007.44

Optimization of humanized IgGs in glycoengineered Pichia pastoris
journal, January 2006

Li, Huijuan; Sethuraman, Natarajan; Stadheim, Terrance A.
Nature Biotechnology, Vol. 24, Issue 2, p. 210-215
https://doi.org/10.1038/nbt1178

Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation
journal, January 2005

Ohya, Tomoshi; Ohyama, Masao; Kobayashi, Kaoru
Biotechnology and Bioengineering, Vol. 90, Issue 7, p. 876-887
https://doi.org/10.1002/bit.20507

A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code
journal, September 2000

Pastrnak, Miro; Magliery, Thomas J.; Schultz, Peter G.
Helvetica Chimica Acta, Vol. 83, Issue 9, p. 2277-2286
https://doi.org/10.1002/1522-2675(20000906)83:9<2277::AID-HLCA2277>3.0.CO;2-L

A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris
journal, August 1998

Shen, Shigang; Sulter, Greitje; Jeffries, Thomas W.
Gene, Vol. 216, Issue 1, p. 93-102
https://doi.org/10.1016/S0378-1119(98)00315-1

High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris
journal, May 1989

Sreekrishna, K.; Nelles, Lynn; Potenz, Rica
Biochemistry, Vol. 28, Issue 9
https://doi.org/10.1021/bi00435a074

A genetically encoded fluorescent amino acid
journal, June 2006

Summerer, D.; Chen, S.; Wu, N.
Proceedings of the National Academy of Sciences, Vol. 103, Issue 26, p. 9785-9789
https://doi.org/10.1073/pnas.0603965103

New Methods Enabling Efficient Incorporation of Unnatural Amino Acids in Yeast
journal, May 2008

Wang, Qian; Wang, Lei
Journal of the American Chemical Society, Vol. 130, Issue 19, p. 6066-6067
https://doi.org/10.1021/ja800894n

Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002

Wang, L.; Zhang, Z.; Brock, A.
Proceedings of the National Academy of Sciences, Vol. 100, Issue 1, p. 56-61
https://doi.org/10.1073/pnas.0234824100

Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion
journal, June 2007

Wang, Kaihang; Neumann, Heinz; Peak-Chew, Sew Y.
Nature Biotechnology, Vol. 25, Issue 7
https://doi.org/10.1038/nbt1314

Genomic Exploration of the Hemiascomycetous Yeasts: 15. Pichia sorbitophila
journal, December 2000

de Montigny, Jacky; Spehner, Catherine; Souciet, Jean-Luc
FEBS Letters, Vol. 487, Issue 1, p. 87-90
https://doi.org/10.1016/S0014-5793(00)02286-9

tRNA genes and retroelements in the yeast genome
journal, February 1998

Hani, J.
Nucleic Acids Research, Vol. 26, Issue 3
https://doi.org/10.1093/nar/26.3.689

Development and Characterization of a Glucagon-Like Peptide 1-Albumin Conjugate: The Ability to Activate the Glucagon-Like Peptide 1 Receptor In Vivo
journal, March 2003

Kim, J. -G.; Baggio, L. L.; Bridon, D. P.
Diabetes, Vol. 52, Issue 3
https://doi.org/10.2337/diabetes.52.3.751

Albumin as a drug carrier: Design of prodrugs, drug conjugates and nanoparticles
journal, December 2008

Kratz, Felix
Journal of Controlled Release, Vol. 132, Issue 3, p. 171-183
https://doi.org/10.1016/j.jconrel.2008.05.010

Unnatural Amino Acid Incorporation into Virus-Like Particles
journal, April 2008

Strable, Erica; Prasuhn, Duane E.; Udit, Andrew K.
Bioconjugate Chemistry, Vol. 19, Issue 4, p. 866-875
https://doi.org/10.1021/bc700390r

PEGylation, successful approach to drug delivery
journal, November 2005

Veronese, Francesco M.; Pasut, Gianfranco
Drug Discovery Today, Vol. 10, Issue 21, p. 1451-1458
https://doi.org/10.1016/S1359-6446(05)03575-0

Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli
journal, July 2002

Chin, Jason W.; Santoro, Stephen W.; Martin, Andrew B.
Journal of the American Chemical Society, Vol. 124, Issue 31, p. 9026-9027
https://doi.org/10.1021/ja027007w

Incorporation of Nonnatural Amino Acids Into Proteins
journal, June 2004

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Annual Review of Biochemistry, Vol. 73, Issue 1
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Title: In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris

Abstract

Citation Formats

Glycoprotein synthesis patent, August 2005

In vivo incorporation of unnatural amino acids patent, May 2006

Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs patent, August 2006

Glycoprotein synthesis patent, October 2006

Glycoprotein synthesis patent, February 2007

Glycoprotein synthesis patent, April 2007

Glycoprotein synthesis patent, May 2007

Glycoprotein synthesis patent, July 2007

Glycoprotein synthesis patent, August 2007

Derivatization of non-natural amino acids and polypeptides patent, June 2008

patent-application, October 2004

Unnatural reactive amino acid genetic code additions patent-application, December 2004

Expanding the eukaryotic genetic code patent-application, January 2005

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells patent-application, June 2005

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application, September 2005

Site-specific incorporation of redox active amino acids into proteins patent-application, October 2005

In vivo incorporation of unnatural amino acids patent-application, November 2005

Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination patent-application, December 2005

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application, March 2006

In vivo incorporation of alkynyl amino acids into proteins in eubacteria patent-application, April 2006

Site-specific labeling of proteins for NMR studies patent-application, May 2006

In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria patent-application, May 2006

Adding photoregulated amino acids to the genetic code patent-application, June 2006

Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof patent-application, July 2006

Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof patent-application, August 2006

Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides patent-application, September 2006

Unnatural reactive amino acid genetic code additions patent-application, November 2006

Site-specific incorporation of redox active amino acids into proteins patent-application, January 2007

Composition of orthogonal glutamyl-trna and aminoacyl-trna synthetase pairs and uses thereof patent-application, January 2007

Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof patent-application, February 2007

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells patent-application, May 2007

In vivo incorporation of unnatural amino acids patent-application, May 2007

Expanding the eukaryotic genetic code patent-application, July 2007

Binding Polypeptides With Diversified And Consensus VH/VL Hypervariable Sequences patent-application, July 2007

Expanding the eukaryotic genetic code patent-application, July 2007

Glycoprotein synthesis patent-application, July 2007

Selective posttranslational modification of phage-displayed polypeptides patent-application, August 2007

Glycoprotein synthesis patent-application, August 2007

Binding Polypeptides and Uses Thereof patent-application, August 2007

Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine patent-application, October 2007

Systems for the expression of orthogonal translation components in eubacterial host cells patent-application, December 2007

Modified Human Growth Hormone patent-application, May 2008

Production of carrier-peptide conjugates using chemically reactive unnatural amino acids patent-application, November 2010

Detailed Analysis Concerning the Biodistribution and Metabolism of Human Calcitonin-Derived Cell-Penetrating Peptides journal, August 2008

Yeast Systems for the Commercial Production of Heterologous Proteins journal, November 1991

Heterologous protein expression in the methylotrophic yeast Pichia pastoris journal, January 2000

Expression of Foreign Genes in the yeast Pichia pastoris book, January 2001

An Improved System for the Generation and Analysis of Mutant Proteins Containing Unnatural Amino Acids in Saccharomyces cerevisiae journal, August 2007

An Expanded Eukaryotic Genetic Code journal, August 2003

Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review journal, April 2006

Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae journal, October 2003

Nucleophilic Catalysis of Oxime Ligation journal, November 2006

Control of protein phosphorylation with a genetically encoded photocaged amino acid journal, October 2007

Optimization of humanized IgGs in glycoengineered Pichia pastoris journal, January 2006

Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation journal, January 2005

A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code journal, September 2000

A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris journal, August 1998

High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris journal, May 1989

A genetically encoded fluorescent amino acid journal, June 2006

New Methods Enabling Efficient Incorporation of Unnatural Amino Acids in Yeast journal, May 2008

Addition of the keto functional group to the genetic code of Escherichia coli journal, December 2002

Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion journal, June 2007

Genomic Exploration of the Hemiascomycetous Yeasts: 15. Pichia sorbitophila journal, December 2000

tRNA genes and retroelements in the yeast genome journal, February 1998

Development and Characterization of a Glucagon-Like Peptide 1-Albumin Conjugate: The Ability to Activate the Glucagon-Like Peptide 1 Receptor In Vivo journal, March 2003

Albumin as a drug carrier: Design of prodrugs, drug conjugates and nanoparticles journal, December 2008

Unnatural Amino Acid Incorporation into Virus-Like Particles journal, April 2008

PEGylation, successful approach to drug delivery journal, November 2005

Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli journal, July 2002

Incorporation of Nonnatural Amino Acids Into Proteins journal, June 2004

Pyrrolysine is not hardwired for cotranslational insertion at UAG codons journal, February 2007

Improved amber and opal suppressor tRNAs for incorporation of unnatural amino acids in vivo. Part 1: Minimizing misacylation journal, August 2007

Adding amino acids to the genetic repertoire journal, December 2005

Glycoprotein synthesis
patent, August 2005

In vivo incorporation of unnatural amino acids
patent, May 2006

Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
patent, August 2006

Glycoprotein synthesis
patent, October 2006

Glycoprotein synthesis
patent, February 2007

Glycoprotein synthesis
patent, April 2007

Glycoprotein synthesis
patent, May 2007

Glycoprotein synthesis
patent, July 2007

Glycoprotein synthesis
patent, August 2007

Derivatization of non-natural amino acids and polypeptides
patent, June 2008

Unnatural reactive amino acid genetic code additions
patent-application, December 2004

Expanding the eukaryotic genetic code
patent-application, January 2005

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, June 2005

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, September 2005

Site-specific incorporation of redox active amino acids into proteins
patent-application, October 2005

In vivo incorporation of unnatural amino acids
patent-application, November 2005

Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
patent-application, December 2005

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, March 2006

In vivo incorporation of alkynyl amino acids into proteins in eubacteria
patent-application, April 2006

Site-specific labeling of proteins for NMR studies
patent-application, May 2006

In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria
patent-application, May 2006

Adding photoregulated amino acids to the genetic code
patent-application, June 2006

Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, July 2006

Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
patent-application, August 2006

Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
patent-application, September 2006

Unnatural reactive amino acid genetic code additions
patent-application, November 2006

Site-specific incorporation of redox active amino acids into proteins
patent-application, January 2007

Composition of orthogonal glutamyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, January 2007

Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, February 2007

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, May 2007

In vivo incorporation of unnatural amino acids
patent-application, May 2007

Expanding the eukaryotic genetic code
patent-application, July 2007

Binding Polypeptides With Diversified And Consensus VH/VL Hypervariable Sequences
patent-application, July 2007

Expanding the eukaryotic genetic code
patent-application, July 2007

Glycoprotein synthesis
patent-application, July 2007

Selective posttranslational modification of phage-displayed polypeptides
patent-application, August 2007

Glycoprotein synthesis
patent-application, August 2007

Binding Polypeptides and Uses Thereof
patent-application, August 2007

Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
patent-application, October 2007

Systems for the expression of orthogonal translation components in eubacterial host cells
patent-application, December 2007

Modified Human Growth Hormone
patent-application, May 2008

Production of carrier-peptide conjugates using chemically reactive unnatural amino acids
patent-application, November 2010

Detailed Analysis Concerning the Biodistribution and Metabolism of Human Calcitonin-Derived Cell-Penetrating Peptides
journal, August 2008

Yeast Systems for the Commercial Production of Heterologous Proteins
journal, November 1991

Heterologous protein expression in the methylotrophic yeast Pichia pastoris
journal, January 2000

Expression of Foreign Genes in the yeast Pichia pastoris
book, January 2001

An Improved System for the Generation and Analysis of Mutant Proteins Containing Unnatural Amino Acids in Saccharomyces cerevisiae
journal, August 2007

An Expanded Eukaryotic Genetic Code
journal, August 2003

Operational strategies, monitoring and control of heterologous protein production in the methylotrophic yeast Pichia pastoris under different promoters: A review
journal, April 2006

Adding Amino Acids with Novel Reactivity to the Genetic Code of Saccharomyces Cerevisiae
journal, October 2003

Nucleophilic Catalysis of Oxime Ligation
journal, November 2006

Control of protein phosphorylation with a genetically encoded photocaged amino acid
journal, October 2007

Optimization of humanized IgGs in glycoengineered Pichia pastoris
journal, January 2006

Optimization of human serum albumin production in methylotrophic yeast Pichia pastoris by repeated fed-batch fermentation
journal, January 2005

A New Orthogonal Suppressor tRNA/Aminoacyl-tRNA Synthetase Pair for Evolving an Organism with an Expanded Genetic Code
journal, September 2000

A strong nitrogen source-regulated promoter for controlled expression of foreign genes in the yeast Pichia pastoris
journal, August 1998

High-level expression, purification, and characterization of recombinant human tumor necrosis factor synthesized in the methylotrophic yeast Pichia pastoris
journal, May 1989

A genetically encoded fluorescent amino acid
journal, June 2006

New Methods Enabling Efficient Incorporation of Unnatural Amino Acids in Yeast
journal, May 2008

Addition of the keto functional group to the genetic code of Escherichia coli
journal, December 2002

Evolved orthogonal ribosomes enhance the efficiency of synthetic genetic code expansion
journal, June 2007

Genomic Exploration of the Hemiascomycetous Yeasts: 15. Pichia sorbitophila
journal, December 2000

tRNA genes and retroelements in the yeast genome
journal, February 1998

Development and Characterization of a Glucagon-Like Peptide 1-Albumin Conjugate: The Ability to Activate the Glucagon-Like Peptide 1 Receptor In Vivo
journal, March 2003

Albumin as a drug carrier: Design of prodrugs, drug conjugates and nanoparticles
journal, December 2008

Unnatural Amino Acid Incorporation into Virus-Like Particles
journal, April 2008

PEGylation, successful approach to drug delivery
journal, November 2005

Addition of p-Azido-L-phenylalanine to the Genetic Code of Escherichia coli
journal, July 2002

Incorporation of Nonnatural Amino Acids Into Proteins
journal, June 2004

Pyrrolysine is not hardwired for cotranslational insertion at UAG codons
journal, February 2007

Improved amber and opal suppressor tRNAs for incorporation of unnatural amino acids in vivo. Part 1: Minimizing misacylation
journal, August 2007

Adding amino acids to the genetic repertoire
journal, December 2005