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Title: In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris

The invention provides orthogonal translation systems for the production of polypeptides comprising unnatural amino acids in methyltrophic yeast such as Pichia pastoris. Methods for producing polypeptides comprising unnatural amino acids in methyltrophic yeast such as Pichia pastoris are also provided.
Inventors:
 [1];  [2]
  1. San Diego, CA
  2. La Jolla, CA
Issue Date:
OSTI Identifier:
1119694
Assignee:
The Scripps Research Institute (La Jolla, CA) DOESC
Patent Number(s):
8,647,840
Application Number:
12/734,995
Contract Number:
FG03-00ER46051
Research Org:
The Scripps Research Institute, La Jolla, CA, USA
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Glycoprotein synthesis
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In vivo incorporation of unnatural amino acids
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Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
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Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
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Site-specific incorporation of redox active amino acids into proteins
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In vivo incorporation of unnatural amino acids
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Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
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Compositions containing, methods involving, and uses of non-natural amino acids and polypeptides
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Unnatural reactive amino acid genetic code additions
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Site-specific incorporation of redox active amino acids into proteins
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Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
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In vivo incorporation of unnatural amino acids
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Expanding the eukaryotic genetic code
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Binding Polypeptides With Diversified And Consensus VH/VL Hypervariable Sequences
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