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Title: Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Abstract

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.

Inventors:
; ;
Issue Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1176455
Patent Number(s):
8216804
Application Number:
11/665,083
Assignee:
Scripps Research Institute
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
ER46051
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Schultz, Peter G., Xie, Jianming, and Zeng, Huaqiang. Orthogonal translation components for the in vivo incorporation of unnatural amino acids. United States: N. p., 2012. Web.
Schultz, Peter G., Xie, Jianming, & Zeng, Huaqiang. Orthogonal translation components for the in vivo incorporation of unnatural amino acids. United States.
Schultz, Peter G., Xie, Jianming, and Zeng, Huaqiang. Tue . "Orthogonal translation components for the in vivo incorporation of unnatural amino acids". United States. https://www.osti.gov/servlets/purl/1176455.
@article{osti_1176455,
title = {Orthogonal translation components for the in vivo incorporation of unnatural amino acids},
author = {Schultz, Peter G. and Xie, Jianming and Zeng, Huaqiang},
abstractNote = {The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2012},
month = {7}
}

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Works referenced in this record:

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