Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Schultz, Peter G.; Alfonta, Lital; Chittuluru, Johnathan R.; Deiters, Alexander; Groff, Dan; Summerer, Daniel; Tsao, Meng -Lin; Wang, Jiangyun; Wu, Ning; Xie, Jianming; Zeng, Huaqiang; Seyedsayamdost, Mohammad; Turner, James

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Title: Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Abstract

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.

Inventors:: Schultz, Peter G.; Alfonta, Lital; Chittuluru, Johnathan R.; Deiters, Alexander; Groff, Dan; Summerer, Daniel; Tsao, Meng -Lin; Wang, Jiangyun; Wu, Ning; Xie, Jianming; Zeng, Huaqiang; Seyedsayamdost, Mohammad; Turner, James

Issue Date:: 2015-08-11

Research Org.:: The Scripps Research Inst., La Jolla, CA (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1209477

Patent Number(s):: 9102932

Application Number:: 13/492,296

Assignee:: The Scripps Research Institute (La Jolla, CA)

Patent Classifications (CPCs):: C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {

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C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C12N15/1058 - {Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms}
C12N9/93 - {Ligases

C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C12P21/00 - Preparation of peptides or proteins
C12P21/02 - having a known sequence of two or more amino acids, e.g. glutathione

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DOE Contract Number:: FG03-00ER45601

Resource Type:: Patent

Resource Relation:: Patent File Date: 2012 Jun 08

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

Citation Formats


                    Schultz, Peter G., Alfonta, Lital, Chittuluru, Johnathan R., Deiters, Alexander, Groff, Dan, Summerer, Daniel, Tsao, Meng -Lin, Wang, Jiangyun, Wu, Ning, Xie, Jianming, Zeng, Huaqiang, Seyedsayamdost, Mohammad, and Turner, James. Orthogonal translation components for the in vivo incorporation of unnatural amino acids.  United States: N. p., 2015. 
        Web.

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                    Schultz, Peter G., Alfonta, Lital, Chittuluru, Johnathan R., Deiters, Alexander, Groff, Dan, Summerer, Daniel, Tsao, Meng -Lin, Wang, Jiangyun, Wu, Ning, Xie, Jianming, Zeng, Huaqiang, Seyedsayamdost, Mohammad, & Turner, James. Orthogonal translation components for the in vivo incorporation of unnatural amino acids.  United States.

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                    Schultz, Peter G., Alfonta, Lital, Chittuluru, Johnathan R., Deiters, Alexander, Groff, Dan, Summerer, Daniel, Tsao, Meng -Lin, Wang, Jiangyun, Wu, Ning, Xie, Jianming, Zeng, Huaqiang, Seyedsayamdost, Mohammad, and Turner, James. Tue .  
        "Orthogonal translation components for the in vivo incorporation of unnatural amino acids".  United States.  https://www.osti.gov/servlets/purl/1209477.

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@article{osti_1209477,

  title        = {Orthogonal translation components for the in vivo incorporation of unnatural amino acids},

  author       = {Schultz, Peter G. and Alfonta, Lital and Chittuluru, Johnathan R. and Deiters, Alexander and Groff, Dan and Summerer, Daniel and Tsao, Meng -Lin and Wang, Jiangyun and Wu, Ning and Xie, Jianming and Zeng, Huaqiang and Seyedsayamdost, Mohammad and Turner, James},

  abstractNote = {The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {2015},

  month        = {8}

}

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Works referenced in this record:

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Glycoprotein synthesis
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Unnatural reactive amino acid genetic code additions
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Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
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Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
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Schultz, Peter; Wang, Lei; Anderson, John Christopher
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Site-specific incorporation of redox active amino acids into proteins
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Alfonta, Lital; Schultz, Peter G.; Zhang, Zhiwen
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Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
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Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
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Schultz, Peter; Wang, Lei; Anderson, John Christopher
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Unnatural reactive amino acid genetic code additions
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Deiters, Alexander; Cropp, T. Ashton; Chin, Jason W.
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Site-specific incorporation of redox active amino acids into proteins
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Alfonta, Lital; Schultz, Peter G.; Zhang, Zhiwen
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Composition of orthogonal glutamyl-trna and aminoacyl-trna synthetase pairs and uses thereof
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Anderson, J. Christopher; Schultz, Peter G.; Santoro, Stephen
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Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
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Anderson, J. Christopher; Wu, Ning; Santoro, Stephen
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Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
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Zhang, Zhiwen; Alfonta, Lital; Schultz, Peter
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Expanding the eukaryotic genetic code
patent-application, July 2007

Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
US Patent Application 11/710655; 20070154952
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Expanding the eukaryotic genetic code
patent-application, July 2007

Chin, Jason; Cropp, T. Ashton; Anderson, J. Christopher
US Patent Application 11/710672; 20070166791
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070166791

Glycoprotein synthesis
patent-application, July 2007

Schultz, Peter; Wang, Lei; Zhang, Zhiwen
US Patent Application 11/580203; 20070172915
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070172915

Selective posttranslational modification of phage-displayed polypeptides
patent-application, August 2007

Tsao, Meng-Lin; Tian, Feng; Schultz, Peter
US Patent Application 11/580223; 20070178448
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Glycoprotein synthesis
patent-application, August 2007

Schultz, Peter; Wang, Lei; Zhang, Zhiwen
US Patent Application 11/520849; 20070184517
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070184517

Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
patent-application, October 2007

Wang, Jiangyun; Schultz, Peter
US Patent Application 11/724039; 20070238152
URL: https://image-ppubs.uspto.gov/dirsearch-public/print/downloadPdf/20070238152

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Orthogonal translation components for the in vivo incorporation of unnatural amino acids

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The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetases that can incorporate unnatural amino acids into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
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Similar Records

Title: Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Abstract

Citation Formats

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli journal, August 2002

An Expanded Eukaryotic Genetic Code journal, August 2003

A Genetically Encoded Photocaged Tyrosine journal, April 2006

Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo journal, September 1997

An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli journal, December 2003

A genetically encoded fluorescent amino acid journal, June 2006

The Genetic Incorporation of a Distance Probe into Proteins in Escherichia coli journal, April 2006

Expanding the Genetic Code journal, January 2005

Adding amino acids to the genetic repertoire journal, December 2005

The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination journal, September 2004

Genetic introduction of a diketone-containing amino acid into proteins journal, October 2006

Expanding the Genetic Code of Escherichia coli journal, April 2001

Amylin antagonist peptides and uses therefor patent, December 1996

Proteomic analysis patent, March 2005

Glycoprotein synthesis patent, August 2005

In vivo incorporation of unnatural amino acids patent, May 2006

Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs patent, August 2006

Glycoprotein synthesis patent, October 2006

Glycoprotein synthesis patent, February 2007

Glycoprotein synthesis patent, April 2007

Glycoprotein synthesis patent, May 2007

Glycoprotein synthesis patent, July 2007

Glycoprotein synthesis patent, August 2007

patent-application, October 2004

Unnatural reactive amino acid genetic code additions patent-application, December 2004

Expanding the eukaryotic genetic code patent-application, January 2005

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells patent-application, June 2005

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application, September 2005

Site-specific incorporation of redox active amino acids into proteins patent-application, October 2005

Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination patent-application, December 2005

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application, March 2006

Site specific incorporation of keto amino acids into proteins patent-application, March 2006

In vivo incorporation of alkynyl amino acids into proteins in eubacteria patent-application, April 2006

Site-specific labeling of proteins for NMR studies patent-application, May 2006

In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria patent-application, May 2006

Adding photoregulated amino acids to the genetic code patent-application, June 2006

Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof patent-application, July 2006

Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof patent-application, August 2006

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs patent-application, October 2006

Unnatural reactive amino acid genetic code additions patent-application, November 2006

Site-specific incorporation of redox active amino acids into proteins patent-application, January 2007

Composition of orthogonal glutamyl-trna and aminoacyl-trna synthetase pairs and uses thereof patent-application, January 2007

Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof patent-application, February 2007

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells patent-application, May 2007

Expanding the eukaryotic genetic code patent-application, July 2007

Expanding the eukaryotic genetic code patent-application, July 2007

Glycoprotein synthesis patent-application, July 2007

Selective posttranslational modification of phage-displayed polypeptides patent-application, August 2007

Glycoprotein synthesis patent-application, August 2007

Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine patent-application, October 2007

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002

An Expanded Eukaryotic Genetic Code
journal, August 2003

A Genetically Encoded Photocaged Tyrosine
journal, April 2006

Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo
journal, September 1997

An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli
journal, December 2003

A genetically encoded fluorescent amino acid
journal, June 2006

The Genetic Incorporation of a Distance Probe into Proteins in Escherichia coli
journal, April 2006

Expanding the Genetic Code
journal, January 2005

Adding amino acids to the genetic repertoire
journal, December 2005

The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination
journal, September 2004

Genetic introduction of a diketone-containing amino acid into proteins
journal, October 2006

Expanding the Genetic Code of Escherichia coli
journal, April 2001

Amylin antagonist peptides and uses therefor
patent, December 1996

Proteomic analysis
patent, March 2005

Glycoprotein synthesis
patent, August 2005

In vivo incorporation of unnatural amino acids
patent, May 2006

Methods and compositions for the production of orthogonal tRNA-aminoacyl tRNA synthetase pairs
patent, August 2006

Glycoprotein synthesis
patent, October 2006

Glycoprotein synthesis
patent, February 2007

Glycoprotein synthesis
patent, April 2007

Glycoprotein synthesis
patent, May 2007

Glycoprotein synthesis
patent, July 2007

Glycoprotein synthesis
patent, August 2007

Unnatural reactive amino acid genetic code additions
patent-application, December 2004

Expanding the eukaryotic genetic code
patent-application, January 2005

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, June 2005

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, September 2005

Site-specific incorporation of redox active amino acids into proteins
patent-application, October 2005

Site specific incorporation of heavy atom-containing unnatural amino acids into proteins for structure determination
patent-application, December 2005

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, March 2006

Site specific incorporation of keto amino acids into proteins
patent-application, March 2006

In vivo incorporation of alkynyl amino acids into proteins in eubacteria
patent-application, April 2006

Site-specific labeling of proteins for NMR studies
patent-application, May 2006

In vivo site-specific incorporation of N-acetyl-galactosamine amino acids in eubacteria
patent-application, May 2006

Adding photoregulated amino acids to the genetic code
patent-application, June 2006

Compositions of orthogonal leucyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, July 2006

Compositions of orthogonal lysyl-tRNA and aminoacyl-tRNA synthetase pairs and uses thereof
patent-application, August 2006

Methods and composition for the production of orthogonal tRNA-aminoacyltRNA synthetase pairs
patent-application, October 2006

Unnatural reactive amino acid genetic code additions
patent-application, November 2006

Site-specific incorporation of redox active amino acids into proteins
patent-application, January 2007

Composition of orthogonal glutamyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, January 2007

Compositions of orthogonal lysyl-trna and aminoacyl-trna synthetase pairs and uses thereof
patent-application, February 2007

Selective incorporation of 5-hydroxytryptophan into proteins in mammalian cells
patent-application, May 2007

Expanding the eukaryotic genetic code
patent-application, July 2007

Expanding the eukaryotic genetic code
patent-application, July 2007

Glycoprotein synthesis
patent-application, July 2007

Selective posttranslational modification of phage-displayed polypeptides
patent-application, August 2007

Glycoprotein synthesis
patent-application, August 2007

Genetically programmed expression of proteins containing the unnatural amino acid phenylselenocysteine
patent-application, October 2007