Orthogonal translation components for the in vivo incorporation of unnatural amino acids
Abstract
The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
- Inventors:
- Issue Date:
- Research Org.:
- The Scripps Research Inst., La Jolla, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1209477
- Patent Number(s):
- 9102932
- Application Number:
- 13/492,296
- Assignee:
- The Scripps Research Institute (La Jolla, CA)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
- DOE Contract Number:
- FG03-00ER45601
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2012 Jun 08
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Schultz, Peter G., Alfonta, Lital, Chittuluru, Johnathan R., Deiters, Alexander, Groff, Dan, Summerer, Daniel, Tsao, Meng -Lin, Wang, Jiangyun, Wu, Ning, Xie, Jianming, Zeng, Huaqiang, Seyedsayamdost, Mohammad, and Turner, James. Orthogonal translation components for the in vivo incorporation of unnatural amino acids. United States: N. p., 2015.
Web.
Schultz, Peter G., Alfonta, Lital, Chittuluru, Johnathan R., Deiters, Alexander, Groff, Dan, Summerer, Daniel, Tsao, Meng -Lin, Wang, Jiangyun, Wu, Ning, Xie, Jianming, Zeng, Huaqiang, Seyedsayamdost, Mohammad, & Turner, James. Orthogonal translation components for the in vivo incorporation of unnatural amino acids. United States.
Schultz, Peter G., Alfonta, Lital, Chittuluru, Johnathan R., Deiters, Alexander, Groff, Dan, Summerer, Daniel, Tsao, Meng -Lin, Wang, Jiangyun, Wu, Ning, Xie, Jianming, Zeng, Huaqiang, Seyedsayamdost, Mohammad, and Turner, James. Tue .
"Orthogonal translation components for the in vivo incorporation of unnatural amino acids". United States. https://www.osti.gov/servlets/purl/1209477.
@article{osti_1209477,
title = {Orthogonal translation components for the in vivo incorporation of unnatural amino acids},
author = {Schultz, Peter G. and Alfonta, Lital and Chittuluru, Johnathan R. and Deiters, Alexander and Groff, Dan and Summerer, Daniel and Tsao, Meng -Lin and Wang, Jiangyun and Wu, Ning and Xie, Jianming and Zeng, Huaqiang and Seyedsayamdost, Mohammad and Turner, James},
abstractNote = {The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {8}
}
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002
- Chin, J. W.; Martin, A. B.; King, D. S.
- Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024
An Expanded Eukaryotic Genetic Code
journal, August 2003
- Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
- Science, Vol. 301, Issue 5635, p. 964-967
A Genetically Encoded Photocaged Tyrosine
journal, April 2006
- Deiters, Alexander; Groff, Dan; Ryu, Youngha
- Angewandte Chemie International Edition, Vol. 45, Issue 17, p. 2728-2731
Engineering a tRNA and aminoacyl-tRNA synthetase for the site-specific incorporation of unnatural amino acids into proteins in vivo
journal, September 1997
- Liu, D. R.; Magliery, T. J.; Pastrnak, M.
- Proceedings of the National Academy of Sciences, Vol. 94, Issue 19
An archaebacteria-derived glutamyl-tRNA synthetase and tRNA pair for unnatural amino acid mutagenesis of proteins in Escherichia coli
journal, December 2003
- Santoro, S. W.
- Nucleic Acids Research, Vol. 31, Issue 23
A genetically encoded fluorescent amino acid
journal, June 2006
- Summerer, D.; Chen, S.; Wu, N.
- Proceedings of the National Academy of Sciences, Vol. 103, Issue 26, p. 9785-9789
The Genetic Incorporation of a Distance Probe into Proteins in Escherichia coli
journal, April 2006
- Tsao, Meng-Lin; Summerer, Daniel; Ryu, Youngha
- Journal of the American Chemical Society, Vol. 128, Issue 14
Expanding the Genetic Code
journal, January 2005
- Wang, Lei; Schultz, Peter G.
- Angewandte Chemie International Edition, Vol. 44, Issue 1, p. 34-66
Adding amino acids to the genetic repertoire
journal, December 2005
- Xie, Jianming; Schultz, Peter G.
- Current Opinion in Chemical Biology, Vol. 9, Issue 6, p. 548-554
The site-specific incorporation of p-iodo-L-phenylalanine into proteins for structure determination
journal, September 2004
- Xie, Jianming; Wang, Lei; Wu, Ning
- Nature Biotechnology, Vol. 22, Issue 10
Genetic introduction of a diketone-containing amino acid into proteins
journal, October 2006
- Zeng, Huaqiang; Xie, Jianming; Schultz, Peter G.
- Bioorganic & Medicinal Chemistry Letters, Vol. 16, Issue 20, p. 5356-5359
Expanding the Genetic Code of Escherichia coli
journal, April 2001
- Wang, L.; Brock, A.; Herberich, B.
- Science, Vol. 292, Issue 5516, p. 498-500