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Title: Orthogonal translation components for the in vivo incorporation of unnatural amino acids

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.
Inventors:
; ; ; ; ; ; ; ; ; ; ; ;
Issue Date:
OSTI Identifier:
1209477
Assignee:
The Scripps Research Institute (La Jolla, CA) CHO
Patent Number(s):
9,102,932
Application Number:
13/492,296
Contract Number:
FG03-00ER45601
Resource Relation:
Patent File Date: 2012 Jun 08
Research Org:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Other works cited in this record:

Addition of a photocrosslinking amino acid to the genetic code of Escherichia coli
journal, August 2002
  • Chin, J. W.; Martin, A. B.; King, D. S.
  • Proceedings of the National Academy of Sciences, Vol. 99, Issue 17, p. 11020-11024
  • DOI: 10.1073/pnas.172226299

An Expanded Eukaryotic Genetic Code
journal, August 2003
  • Chin, Jason W.; Cropp, T. Ashton; Anderson, J. Christopher
  • Science, Vol. 301, Issue 5635, p. 964-967
  • DOI: 10.1126/science.1084772

A Genetically Encoded Photocaged Tyrosine
journal, April 2006
  • Deiters, Alexander; Groff, Dan; Ryu, Youngha
  • Angewandte Chemie International Edition, Vol. 45, Issue 17, p. 2728-2731
  • DOI: 10.1002/anie.200600264

A genetically encoded fluorescent amino acid
journal, June 2006
  • Summerer, D.; Chen, S.; Wu, N.
  • Proceedings of the National Academy of Sciences, Vol. 103, Issue 26, p. 9785-9789
  • DOI: 10.1073/pnas.0603965103

Expanding the Genetic Code
journal, January 2005
  • Wang, Lei; Schultz, Peter G.
  • Angewandte Chemie International Edition, Vol. 44, Issue 1, p. 34-66
  • DOI: 10.1002/anie.200460627

Adding amino acids to the genetic repertoire
journal, December 2005
  • Xie, Jianming; Schultz, Peter G.
  • Current Opinion in Chemical Biology, Vol. 9, Issue 6, p. 548-554
  • DOI: 10.1016/j.cbpa.2005.10.011

Genetic introduction of a diketone-containing amino acid into proteins
journal, October 2006
  • Zeng, Huaqiang; Xie, Jianming; Schultz, Peter G.
  • Bioorganic & Medicinal Chemistry Letters, Vol. 16, Issue 20, p. 5356-5359
  • DOI: 10.1016/j.bmcl.2006.07.094

Expanding the Genetic Code of Escherichia coli
journal, April 2001

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