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Title: Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Abstract

The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.

Inventors:
; ; ; ; ; ; ; ; ; ; ; ;
Issue Date:
Research Org.:
The Scripps Research Inst., La Jolla, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1209477
Patent Number(s):
9102932
Application Number:
13/492,296
Assignee:
The Scripps Research Institute (La Jolla, CA)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG03-00ER45601
Resource Type:
Patent
Resource Relation:
Patent File Date: 2012 Jun 08
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Schultz, Peter G., Alfonta, Lital, Chittuluru, Johnathan R., Deiters, Alexander, Groff, Dan, Summerer, Daniel, Tsao, Meng -Lin, Wang, Jiangyun, Wu, Ning, Xie, Jianming, Zeng, Huaqiang, Seyedsayamdost, Mohammad, and Turner, James. Orthogonal translation components for the in vivo incorporation of unnatural amino acids. United States: N. p., 2015. Web.
Schultz, Peter G., Alfonta, Lital, Chittuluru, Johnathan R., Deiters, Alexander, Groff, Dan, Summerer, Daniel, Tsao, Meng -Lin, Wang, Jiangyun, Wu, Ning, Xie, Jianming, Zeng, Huaqiang, Seyedsayamdost, Mohammad, & Turner, James. Orthogonal translation components for the in vivo incorporation of unnatural amino acids. United States.
Schultz, Peter G., Alfonta, Lital, Chittuluru, Johnathan R., Deiters, Alexander, Groff, Dan, Summerer, Daniel, Tsao, Meng -Lin, Wang, Jiangyun, Wu, Ning, Xie, Jianming, Zeng, Huaqiang, Seyedsayamdost, Mohammad, and Turner, James. Tue . "Orthogonal translation components for the in vivo incorporation of unnatural amino acids". United States. https://www.osti.gov/servlets/purl/1209477.
@article{osti_1209477,
title = {Orthogonal translation components for the in vivo incorporation of unnatural amino acids},
author = {Schultz, Peter G. and Alfonta, Lital and Chittuluru, Johnathan R. and Deiters, Alexander and Groff, Dan and Summerer, Daniel and Tsao, Meng -Lin and Wang, Jiangyun and Wu, Ning and Xie, Jianming and Zeng, Huaqiang and Seyedsayamdost, Mohammad and Turner, James},
abstractNote = {The invention relates to orthogonal pairs of tRNAs and aminoacyl-tRNA synthetase that can incorporate unnatural amino acid into proteins produced in eubacterial host cells such as E. coli, or in a eukaryotic host such as a yeast cell. The invention provides, for example but not limited to, novel orthogonal synthetases, methods for identifying and making the novel synthetases, methods for producing proteins containing unnatural amino acids, and translation systems.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {8}
}

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Works referenced in this record:

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An Expanded Eukaryotic Genetic Code
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