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Title: Host cells and methods for production of isobutanol

Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
Inventors:
; ; ; ; ; ; ; ; ; ;
Issue Date:
OSTI Identifier:
1303694
Assignee:
Butamax Advanced Biofuels LLC (Wilmington, DE) ARPA-E
Patent Number(s):
9,422,582
Application Number:
13/840,676
Contract Number:
AR0000006
Resource Relation:
Patent File Date: 2013 Mar 15
Research Org:
Butamax Advanced Biofuels LLC, Wilmington, DE (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES

Works referenced in this record:

Solventogenic enzymes of Clostridium acetobutylicum: catalytic properties, genetic organization, and transcriptional regulation
journal, October 1995

New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation
journal, June 1998
  • Dürre, P.
  • Applied Microbiology and Biotechnology, Vol. 49, Issue 6, p. 639-648
  • DOI: 10.1007/s002530051226

Technologies for butanol recovery integrated with fermentations
journal, March 1992
  • Groot, W. J.; van der Lans, R. G. J. M.; Luyben, K. Ch. A. M.
  • Process Biochemistry, Vol. 27, Issue 2, p. 61-75
  • DOI: 10.1016/0032-9592(92)80012-R

SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling
journal, January 1997

Improved methods for building protein models in electron density maps and the location of errors in these models
journal, March 1991
  • Jones, T. A.; Zou, J. Y.; Cowan, S. W.
  • Acta Crystallographica Section A Foundations of Crystallography, Vol. 47, Issue 2, p. 110-119
  • DOI: 10.1107/S0108767390010224

Effect of aliphatic alcohols on growth and degree of saturation of membrane lipids in Acinetobacter calcoaceticus
journal, March 2003
  • Kabelitz, Nadja; Santos, Pedro M.; Heipieper, Hermann J.
  • FEMS Microbiology Letters, Vol. 220, Issue 2, p. 223-227
  • DOI: 10.1016/S0378-1097(03)00103-4

One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000
  • Datsenko, K. A.; Wanner, B. L.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
  • DOI: 10.1073/pnas.120163297

Fusel Alcohols Production in Beer Fermentation Processes
journal, January 1994

Modular expression and secretion vectors for Bacillus subtilis
journal, May 1992

High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening
journal, December 1993

Plasmid vectors for Gram-positive bacteria switching from high to low copy number
journal, January 1996

Sequences of versatile broad-host-range vectors of the RK2 family
journal, July 2003

Crystal Structure of E.coli Alcohol Dehydrogenase YqhD: Evidence of a Covalently Modified NADP Coenzyme
journal, September 2004
  • Sulzenbacher, Gerlind; Alvarez, Karine; van den Heuvel, Robert H. H.
  • Journal of Molecular Biology, Vol. 342, Issue 2, p. 489-502
  • DOI: 10.1016/j.jmb.2004.07.034

CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994
  • Thompson, Julie D.; Higgins, Desmond G.; Gibson, Toby J.
  • Nucleic Acids Research, Vol. 22, Issue 22, p. 4673-4680
  • DOI: 10.1093/nar/22.22.4673

Functional Analysis of Three Plasmids from Lactobacillus plantarum
journal, March 2005

Purification and characterization of a fusion protein of plant acetohydroxy acid synthase and acetohydroxy acid isomeroreductase
journal, May 1997

Guerbet condensation of methanol with n-propanol to isobutyl alcohol over heterogeneous bifunctional catalysts based on Mg–Al mixed oxides partially substituted by different metal components
journal, May 2005
  • Carlini, Carlo; Marchionna, Mario; Noviello, Marilena
  • Journal of Molecular Catalysis A: Chemical, Vol. 232, Issue 1-2, p. 13-20
  • DOI: 10.1016/j.molcata.2004.12.037

Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension
journal, April 1989

An Investigation of the Metabolism of Valine to Isobutyl Alcohol in Saccharomyces cerevisiae
journal, October 1998
  • Dickinson, J. Richard; Harrison, Scott J.; Hewlins, Michael J. E.
  • Journal of Biological Chemistry, Vol. 273, Issue 40, p. 25751-25756
  • DOI: 10.1074/jbc.273.40.25751

Towards a new interaction enzyme:coenzyme
journal, April 2005
  • Martínez-Júlvez, Marta; Tejero, Jesús; R. Peregrina, José
  • Biophysical Chemistry, Vol. 115, Issue 2-3, p. 219-224
  • DOI: 10.1016/j.bpc.2004.12.034

Crystal Structure of Class I Acetohydroxy Acid Isomeroreductase from Pseudomonas aeruginosa
journal, April 2003

NADP-Dependent enzymes. I: Conserved stereochemistry of cofactor binding
journal, May 1997

The X-ray structure of Brassica napus β-keto acyl carrier protein reductase and its implications for substrate binding and catalysis
journal, April 2000

Prediction of the occurrence of the ADP-binding βαβ-fold in proteins, using an amino acid sequence fingerprint
journal, January 1986
  • Wierenga, Rik K.; Terpstra, Peter; Hol, Wim G. J.
  • Journal of Molecular Biology, Vol. 187, Issue 1, p. 101-107
  • DOI: 10.1016/0022-2836(86)90409-2

General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH
journal, June 2013
  • Brinkmann-Chen, S.; Flock, T.; Cahn, J. K. B.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 27, p. 10946-10951
  • DOI: 10.1073/pnas.1306073110

A single amino acid substitution in lactate dehydrogenase improves the catalytic efficiency with an alternative coenzyme
journal, January 1990
  • Feeney, Raymond; Clarke, Anthony R.; Holbrook, J. John
  • Biochemical and Biophysical Research Communications, Vol. 166, Issue 2, p. 667-672
  • DOI: 10.1016/0006-291X(90)90861-G

Fast and sensitive multiple sequence alignments on a microcomputer
journal, April 1989

A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum
journal, April 2006

Characterization of short-chain dehydrogenase/reductase homologues of Escherichia coli (YdfG) and Saccharomyces cerevisiae (YMR226C)
journal, January 2003
  • Fujisawa, Hisae; Nagata, Shinji; Misono, Haruo
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1645, Issue 1, p. 89-94
  • DOI: 10.1016/S1570-9639(02)00533-2

Strain engineering for stereoselective bioreduction of dicarbonyl compounds by yeast reductases
journal, April 2005

Enzymatic ketone reduction: mapping the substrate profile of a short-chain alcohol dehydrogenase (YMR226c) from Saccharomyces cerevisiae
journal, August 2007

Secoiridoid and flavonoid glycosides from Gonocaryum calleryanum
journal, May 1995

Stoichiometry and compartmentation of NADH metabolism in Saccharomyces cerevisiae
journal, January 2001