Host cells and methods for production of isobutanol
Abstract
Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.
- Inventors:
- Issue Date:
- Research Org.:
- Butamax Advanced Biofuels LLC, Wilmington, DE (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1351895
- Patent Number(s):
- 9422581
- Application Number:
- 13/840,649
- Assignee:
- Butamax Advanced Biofuels LLC (Wilmington, DE)
- Patent Classifications (CPCs):
-
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
C - CHEMISTRY C12 - BIOCHEMISTRY C12P - FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {
- DOE Contract Number:
- AR0000006
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2013 Mar 15
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 09 BIOMASS FUELS
Citation Formats
Anthony, Larry Cameron, He, Hongxian, Huang, Lixuan Lisa, Okeefe, Daniel P., Kruckeberg, Arthur Leo, Li, Yougen, Maggio-Hall, Lori Ann, McElvain, Jessica, Nelson, Mark J., Patnaik, Ranjan, and Rothman, Steven Cary. Host cells and methods for production of isobutanol. United States: N. p., 2016.
Web.
Anthony, Larry Cameron, He, Hongxian, Huang, Lixuan Lisa, Okeefe, Daniel P., Kruckeberg, Arthur Leo, Li, Yougen, Maggio-Hall, Lori Ann, McElvain, Jessica, Nelson, Mark J., Patnaik, Ranjan, & Rothman, Steven Cary. Host cells and methods for production of isobutanol. United States.
Anthony, Larry Cameron, He, Hongxian, Huang, Lixuan Lisa, Okeefe, Daniel P., Kruckeberg, Arthur Leo, Li, Yougen, Maggio-Hall, Lori Ann, McElvain, Jessica, Nelson, Mark J., Patnaik, Ranjan, and Rothman, Steven Cary. Tue .
"Host cells and methods for production of isobutanol". United States. https://www.osti.gov/servlets/purl/1351895.
@article{osti_1351895,
title = {Host cells and methods for production of isobutanol},
author = {Anthony, Larry Cameron and He, Hongxian and Huang, Lixuan Lisa and Okeefe, Daniel P. and Kruckeberg, Arthur Leo and Li, Yougen and Maggio-Hall, Lori Ann and McElvain, Jessica and Nelson, Mark J. and Patnaik, Ranjan and Rothman, Steven Cary},
abstractNote = {Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {8}
}
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Works referenced in this record:
Process for amplifying nucleic acid sequences
patent, July 1987
- Mullis, Kary B.
- US Patent Document 4,683,202
Process for extractive fermentation
patent, September 1989
- Kollerup, Finn; Daugulis, Andrew J.
- US Patent Document 4,865,973
Nucleic acid coding for an .alpha.-acetolactate synthase from lactococcus and its applications
patent, July 1997
- Ehrlich, Stanislav; Godon, Jean-Jacques; Renault, Pierre
- US Patent Document 5,643,779
Method for the production of &rgr;-Hydroxybenzoate in species of pseudomonas and agrobacterium
patent, July 2003
- Ben-Bassat, Arie; Cattermole, Monica; Gatenby, Anthony A.
- US Patent Document 6,586,229
Increased cell resistance to toxic organic substances
patent, November 2005
- Papoutsakis, Eleftherios T.; Tomas, Christopher A.; Tesic, Marija
- US Patent Document 6,960,465
Solvent tolerant microorganisms and methods of isolation
patent, June 2009
- Bramucci, Michael G.; Nagarajan, Vasantha; Sedkova, Natalia
- US Patent Document 7,541,173
Solvent tolerant microorganisms and methods of isolation
patent, February 2010
- Bramucci, Michael G.; Nagarajan, Vasantha; Sedkova, Natalia
- US Patent Document 7,659,104
Fermentive production of four carbon alcohols
patent, December 2010
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 7,851,188
Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
patent, March 2011
- Liao, Der-Ing; Nelson, Mark J.; Bramucci, Michael G.
- US Patent Document 7,910,342
Fermentive production of four carbon alcohols
patent, August 2011
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 7,993,889
Solvent tolerant microorganisms
patent, September 2011
- Bramucci, Michael G.; Kane, Helene M. A.; Nagarajan, Vasantha
- US Patent Document 8,017,364
Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
patent, September 2011
- Dundon, Catherine Asleson; Aristidou, Aristos; Hawkins, Andrew
- US Patent Document 8,017,376
Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
patent, December 2011
- Dundon, Catherine Asleson; Aristidou, Aristos; Hawkins, Andrew
- US Patent Document 8,071,358
Engineered microorganisms capable of producing target compounds under anaerobic conditions
patent, January 2012
- Buelter, Thomas; Meinhold, Peter; Feldman, Reid M. Renny
- US Patent Document 8,097,440
Recovery of higher alcohols from dilute aqueous solutions
patent, January 2012
- Evanko, William A.; Eyal, Aharon; Glassner, David A.
- US Patent Document 8,101,808
Ketol-acid reductoisomerase using NADH
patent, March 2012
- Li, Yougen; Liao, Der-Ing; Nelson, Mark J.
- US Patent Document 8,129,162
Reduced by-product accumulation for improved production of isobutanol
patent, March 2012
- Buelter, Thomas; Hawkins, Andrew; Porter-Scheinman, Stephanie
- US Patent Document 8,133,715
Reduced by-product accumulation for improved production of isobutanol
patent, April 2012
- Buelter, Thomas; Hawkins, Andrew; Porter-Scheinman, Stephanie
- US Patent Document 8,153,415
Reduced by-product accumulation for improved production of isobutanol
patent, April 2012
- Lies, Doug; Porter-Scheinman, Stephanie; Kelly, Julie
- US Patent Document 8,158,404
Fermentive production of four carbon alcohols
patent, May 2012
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,178,328
Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
patent, May 2012
- Bramucci, Michael G.; Eliot, Andrew C.; Maggio-Hall, Lori Ann
- US Patent Document 8,188,250
Production of 2-butanol and 2-butanone employing aminobutanol phosphate phospholyase
patent, June 2012
- Eliot, Andrew C.; Nagarajan, Vasantha; Huang, Lixuan Lisa
- US Patent Document 8,206,970
Ketol-acid reductoisomerase using NADH
patent, July 2012
- Li, Yougen; Liao, Der-Ing; Nelson, Mark J.
- US Patent Document 8,222,017
Cytosolic isobutanol pathway localization for the production of isobutanol
patent, July 2012
- Urano, Jun; Dundon, Catherine Asleson
- US Patent Document 8,232,089
Recombinant yeast host cell with Fe-S cluster proteins and methods of using thereof
patent, August 2012
- Anthony, Larry Cameron; Flint, Dennis; Suh, Wonchul
- US Patent Document 8,241,878
Fermentive production of four carbon alcohols
patent, September 2012
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,273,558
Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids
patent, September 2012
- Dundon, Catherine Asleson; Aristidou, Aristos; Hawkins, Andrew
- US Patent Document 8,273,565
Fermentive production of four carbon alcohols
patent, October 2012
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,283,144
Production of four carbon alcohols using improved strain
patent, February 2013
- Larossa, Robert A.; Smulski, Dana R.; Nagarajan, Vasantha
- US Patent Document 8,372,612
Yeast strain for production of four carbon alcohols
patent, March 2013
- Larossa, Robert A.
- US Patent Document 8,389,252
Enhanced pyruvate to 2,3-butanediol conversion in lactic acid bacteria
patent, June 2013
- Paul, Brian James
- US Patent Document 8,455,224
Yeast with increased butanol tolerance involving high osmolarity/glycerol response pathway
patent, June 2013
- Bramucci, Michael G.; Larossa, Robert A.; Smulski, Dana R.
- US Patent Document 8,455,225
Yeast organism producing isobutanol at a high yield
patent, June 2013
- Feldman, Reid M. Renny; Gunawardena, Uvini; Urano, Jun
- US Patent Document 8,455,239
Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
patent, June 2013
- Anthony, Larry Cameron
- US Patent Document 8,465,964
Strain comprising increased expression of a CFA coding region for butanol production
patent, August 2013
- Flint, Dennis; Larossa, Robert A.; Nagarajan, Vasantha
- US Patent Document 8,518,678
Yeast with increased butanol tolerance involving filamentous growth response
patent, October 2013
- Bramucci, Michael G.; Larossa, Robert A.; Singh, Manjari
- US Patent Document 8,557,562
Yeast with increased butanol tolerance involving a multidrug efflux pump gene
patent, December 2013
- Van Dyk, Tina K.
- US Patent Document 8,614,085
Enhanced dihydroxy-acid dehydratase activity in lactic acid bacteria
patent, January 2014
- Paul, Brian James; Suh, Wonchul
- US Patent Document 8,637,281
Expression of hexose kinase in recombinant host cells
patent, January 2014
- Anthony, Larry Cameron; Kruckeberg, Arthur Leo
- US Patent Document 8,637,289
Strain for butanol production with increased membrane unsaturated trans fatty acids
patent, February 2014
- Flint, Dennis; Van Dyk, Tina K.
- US Patent Document 8,652,823
Enhanced pyruvate to acetolactate conversion in yeast
patent, March 2014
- Anthony, Larry Cameron; Maggio-Hall, Lori Ann
- US Patent Document 8,669,094
Butanol dehydrogenase enzyme from the bacterium Achromobacter xylosoxidans
patent, April 2014
- Bramucci, Michael G.; Eliot, Andrew C.; Maggio-Hall, Lori Ann
- US Patent Document 8,691,540
Fermentive production of four carbon alcohols
patent, May 2014
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,735,114
Alcohol dehydrogenases (ADH) useful for fermentive production of lower alkyl alcohols
patent, July 2014
- Satagopan, Sriram; O'Keefe, Daniel P.; Gude, Janardhan
- US Patent Document 8,765,433
Increased production of isobutanol in yeast with reduced mitochondrial amino acid biosynthesis
patent, July 2014
- Anthony, Larry Cameron
- US Patent Document 8,785,166
Yeast with increased butanol tolerance involving cell wall integrity pathway
patent, August 2014
- Bramucci, Michael G.; Larossa, Robert A.; Smulski, Dana R.
- US Patent Document 8,795,992
Production of isobutanol in yeast mitochondria
patent, September 2014
- Anthony, Larry Cameron; Huang, Lixuan Lisa; Ye, Rick W.
- US Patent Document 8,828,694
Fermentive production of four carbon alcohols
patent, September 2014
- Donaldson, Gail K.; Eliot, Andrew C.; Maggio-Hall, Lori Ann
- US Patent Document 8,828,704
Recombinant host cells comprising phosphoketolases
patent, October 2014
- Dauner, Michael; Maggio-Hall, Lori Ann; Tomb, Jean-Francois
- US Patent Document 8,871,488
Fermentive production of four carbon alcohols
patent, November 2014
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,889,385
Ketol-acid reductoisomerase using NADH
patent, November 2014
- Li, Yougen; Liao, Der-Ing; Nelson, Mark J.
- US Patent Document 8,895,307
Engineering resistance to aliphatic alcohols
patent, December 2014
- Alsaker, Keith; Grenfell-Lee, Daniel; Hudson, Michael
- US Patent Document 8,906,666
Ketol-acid reductoisomerase using NADH
patent, December 2014
- Li, Yougen; Liao, Der-Ing; Nelson, Mark J.
- US Patent Document 8,911,981
Yeast strain for production of four carbon alcohols
patent, January 2015
- Larossa, Robert A.
- US Patent Document 8,940,511
Production of isobutanol by a microorganism with enhanced acetolactate synthase activity
patent, February 2015
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,945,859
Ketol-acid reductoisomerase using NADH
patent, February 2015
- Li, Yougen; Liao, Der-Ing; Nelson, Mark J.
- US Patent Document 8,945,899
Fermentive production of four carbon alcohols
patent, February 2015
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Document 8,951,774
Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases
patent, February 2015
- Flint, Dennis; Rothman, Steven Cary; Suh, Wonchul
- US Patent Document 8,951,937
Enhanced pyruvate to acetolactate conversion in yeast
patent, February 2015
- Anthony, Larry Cameron; Maggio-Hall, Lori Ann
- US Patent Document 8,956,850
Recombinant host cell comprising a diol dehydratase
patent, February 2015
- Donaldson, Gail K.; Eliot, Andrew C.; Nagarajan, Vasantha
- US Patent Document 8,962,298
Enhanced pyruvate to acetolactate conversion in yeast
patent, March 2015
- Anthony, Larry Cameron; Maggio-Hall, Lori Ann
- US Patent Document 8,969,065
Fermentive production of four carbon alcohols
patent, March 2015
- Donaldson, Gail K.; Eliot, Andrew C.; Nagarajan, Vasantha
- US Patent Document 8,980,612
Method for producing ethanol using raw starch
patent-application, November 2004
- Lewis, Stephen M.; Van Hulzen, Shon Erron; Finck, John Michael
- US Patent Application 10/798226; 20040234649
Treatment of biomass to obtain fermentable sugars
patent-application, February 2007
- Dunson, Jr., James B.; Elander, Richard T.; Tucker, III, Melvin P.
- US Patent Application 11/402757; 20070031918
Fermentative Production Of Four Carbon Alcohols
patent-application, April 2007
- Donaldson, Gail K.; Eliot, Andrew C.; Flint, Dennis
- US Patent Application 11/586315; 20070092957
Fermentive Production Of Four Carbon Alcohols
patent-application, July 2008
- Donaldson, Gail K.; Huang, Lixuan Lisa; Maggio-Hall, Lori Ann
- US Patent Application 11/527995; 20080182308
Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes
patent-application, October 2008
- Liao, Der-Ing; Nelson, Mark J.; Bramucci, Michael G.
- US Patent Application 12/103844; 20080261230
Identification and use of Bacterial [2FE-2S] Dihydroxy-acid Dehydratases
patent-application, April 2010
- Flint, Dennis; Rothman, Steven Cary; Suh, Wonchul
- US Patent Application 12/569636; 20100081154
Enhanced Iron-sulfur Cluster Formation for Increased Dihydroxy-Acid Dehydratase Activity in Lactic Acid Bacteria
patent-application, April 2010
- Paul, Brain James; Suh, Wonchul
- US Patent Application 12/569103; 20100081182
Carbon Pathway Optimized Production Hosts for the Production of Isobutanol
patent-application, May 2010
- Anthony, Larry Cameron; Dauner, Michael; Donaldson, Gail K.
- US Patent Application 12/606410; 20100120105
Engineered microorganisms capable of producing target compounds under anaerobic conditions
patent-application, June 2021
- Buelter, Thomas; Meinhold, Peter; Feldman, Reid M. Renny
- US Patent Application 12/610784; 20100143997
Cytosolic Isobutanol Pathway Localization for the Production of Isobutanol
patent-application, March 2011
- Urano, Jun; Dundon, Catherine Asleson; Meinhold, Peter
- US Patent Application 12/855276; 20110076733
Bacterial Strains for Butanol Production
patent-application, August 2011
- Euler, Lori Jean; Flint, Dennis; Paul, Brian James
- US Patent Application 12/899760; 20110195505
Fermentive Production of Isobutanol Using Highly Effective Ketol-acid Reductoisomerase Enzymes
patent-application, October 2011
- Nagarajan, Vasantha; Paul, Brian James; Suh, Wonchul
- US Patent Application 12/893077; 20110244536
Engineering Resistance to Aliphatic Alcohols
patent-application, March 2012
- Alsaker, Keith; Grenfell-Lee, Daniel; Hudson, Michael
- US Patent Application 13/214310; 20120058541
Fermentive Production of Four Carbon Alcohols
patent-application, August 2012
- Donaldson, Gail K.; Eliot, Andrew C.; Nagarajan, Vasantha
- US Patent Application 13/281872; 20120196341
Lignocellulosic Hydrolysates as Feedstocks for Isobutanol Fermentation
patent-application, February 2013
- Dobson, Ian David; Kruckeberg, Arthur Leo
- US Patent Application 13/525032; 20130035515
Acetate Supplementation of Medium for Butanologens
patent-application, September 2013
- Maggio-Hall, Lori Ann
- US Patent Application 13/833369; 20130252296
Host Cells and Methods for Production of Isobutanol
patent-application, January 2014
- Anthony, Larry Cameron; He, Hongxian; Huang, Lixuan Lisa
- US Patent Application 13/840649; 20140030782
Host Cells and Methods for Production of Isobutanol
patent-application, January 2014
- Anthony, Larry Cameron; He, Hongxian; Huang, Lixuan Lisa
- US Patent Application 13/840676; 20140030783
Activity of FE-S Cluster Requiring Proteins
patent-application, February 2014
- Flint, Dennis; Paul, Brian James; Ye, Rick W.
- US Patent Application 13/837921; 20140038263
Dhad Variants for Butanol Production
patent-application, July 2014
- Maggio-Hall, Loriann Ann; Paul, Brian James; Rothman, Steven Cary
- US Patent Application 14/142,398; 2014/0186910 Al
Continuous direct solvent extraction of butanol in a fermenting fluidized-bed bioreactor with immobilizedClostridium acetobutylicum
journal, September 1993
- Davison, Brian H.; Thompson, James E.
- Applied Biochemistry and Biotechnology, Vol. 39-40, Issue 1
Identification of FRA1 and FRA2 as Genes Involved in Regulating the Yeast Iron Regulon in Response to Decreased Mitochondrial Iron-Sulfur Cluster Synthesis
journal, February 2008
- Kumánovics, Attila; Chen, Opal S.; Li, Liangtao
- Journal of Biological Chemistry, Vol. 283, Issue 16, p. 10276-10286
Contribution of VanY D,D-carboxypeptidase to glycopeptide resistance in Enterococcus faecalis by hydrolysis of peptidoglycan precursors.
journal, September 1994
- Arthur, M.; Depardieu, F.; Snaith, H. A.
- Antimicrobial Agents and Chemotherapy, Vol. 38, Issue 9
Oxalyl hydroxamates as reaction-intermediate analogs for ketol-acid reductoisomerase
journal, March 1990
- Aulabaugh, Ann; Schloss, John V.
- Biochemistry, Vol. 29, Issue 11
The crystal structure of plant acetohydroxy acid isomeroreductase complexed with NADPH, two magnesium ions and a herbicidal transition state analog determined at 1.65Aresolution
journal, June 1997
- Biou, V.
- The EMBO Journal, Vol. 16, Issue 12
Targeted Disruption of the kstD Gene Encoding a 3-Ketosteroid Delta 1-Dehydrogenase Isoenzyme of Rhodococcus erythropolis Strain SQ1
journal, May 2000
- van der Geize, R.; Hessels, G. I.; van Gerwen, R.
- Applied and Environmental Microbiology, Vol. 66, Issue 5
Mechanism of ketol acid reductoisomerase. Steady-state analysis and metal ion requirement
journal, January 1989
- Chunduru, Srinivas K.; Mrachko, Gregory T.; Calvo, K. C.
- Biochemistry, Vol. 28, Issue 2
Mycobacterium sp.,Rhodococcus erythropolis, andPseudomonas putida behavior in the presence of organic solvents
journal, January 2004
- De Carvalho, Carla C. C. R.; Da Cruz, Alexandra A. R. L.; Pons, Marie-N�elle
- Microscopy Research and Technique, Vol. 64, Issue 3
Biochemical and molecular characterization of α-ketoisovalerate decarboxylase, an enzyme involved in the formation of aldehydes from amino acids by Lactococcus lactis
journal, September 2004
- Plaza, Marta; Fernández de Palencia, Pilar; Peláez, Carmen
- FEMS Microbiology Letters, Vol. 238, Issue 2
Ethanol production from cellulose by coupled saccharification/fermentation usingSaccharomyces cerevisiae and cellulase complex fromSclerotiun rolfsii UV-8 mutant
journal, September 1992
- Deshpande, Mukund V.
- Applied Biochemistry and Biotechnology, Vol. 36, Issue 3, p. 227-234
Solventogenic enzymes of Clostridium acetobutylicum: catalytic properties, genetic organization, and transcriptional regulation
journal, January 1995
- Dürre, P.
- FEMS Microbiology Reviews, Vol. 17, Issue 3
New insights and novel developments in clostridial acetone/butanol/isopropanol fermentation
journal, June 1998
- Dürre, P.
- Applied Microbiology and Biotechnology, Vol. 49, Issue 6, p. 639-648
Characterization of Ypr1p fromSaccharomyces cerevisiae as a 2-methylbutyraldehyde reductase
journal, January 2002
- Ford, Gordon; Ellis, Elizabeth M.
- Yeast, Vol. 19, Issue 12
pAM401-Based Shuttle Vectors That Enable Overexpression of Promoterless Genes and One-Step Purification of Tag Fusion Proteins Directly from Enterococcus faecalis
journal, March 2001
- Fujimoto, S.; Ike, Y.
- Applied and Environmental Microbiology, Vol. 67, Issue 3, p. 1262-1267
Technologies for butanol recovery integrated with fermentations
journal, March 1992
- Groot, W. J.; van der Lans, R. G. J. M.; Luyben, K. Ch. A. M.
- Process Biochemistry, Vol. 27, Issue 2, p. 61-75
SWISS-MODEL and the Swiss-Pdb Viewer: An environment for comparative protein modeling
journal, January 1997
- Guex, Nicolas; Peitsch, Manuel C.
- Electrophoresis, Vol. 18, Issue 15, p. 2714-2723
Improved methods for building protein models in electron density maps and the location of errors in these models
journal, March 1991
- Jones, T. A.; Zou, J. Y.; Cowan, S. W.
- Acta Crystallographica Section A Foundations of Crystallography, Vol. 47, Issue 2, p. 110-119
Effect of aliphatic alcohols on growth and degree of saturation of membrane lipids in Acinetobacter calcoaceticus
journal, March 2003
- Kabelitz, Nadja; Santos, Pedro M.; Heipieper, Hermann J.
- FEMS Microbiology Letters, Vol. 220, Issue 2, p. 223-227
One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products
journal, May 2000
- Datsenko, K. A.; Wanner, B. L.
- Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6640-6645
A small cryptic plasmid from Rhodococcus erythropolis: characterization and utility for gene expression
journal, July 2003
- Kostichka, K.; Tao, L.; Bramucci, M.
- Applied Microbiology and Biotechnology, Vol. 62, Issue 1
Hidden Markov Models in Computational Biology
journal, February 1994
- Krogh, Anders; Brown, Michael; Mian, I. Saira
- Journal of Molecular Biology, Vol. 235, Issue 5
Characterization of the Saccharomyces cerevisiae YMR318C (ADH6) gene product as a broad specificity NADPH-dependent alcohol dehydrogenase: relevance in aldehyde reduction
journal, January 2002
- Larroy, Carol; Fernandez, M. Rosario; Gonzalez, Eva
- Biochemical Journal, Vol. 361, Issue 1, p. 163-172
New thermosensitive plasmid for gram-positive bacteria.
journal, September 1992
- Maguin, E.; Duwat, P.; Hege, T.
- Journal of Bacteriology, Vol. 174, Issue 17, p. 5633-5638
Modular expression and secretion vectors for Bacillus subtilis
journal, May 1992
- Nagarajan, Vasantha; Albertson, Helene; Chen, Mario
- Gene, Vol. 114, Issue 1, p. 121-126
Construction of Improved Temperature-Sensitive and Mobilizable Vectors and Their Use for Constructing Mutations in the Adhesin-Encoding acm Gene of Poorly Transformable Clinical Enterococcus faecium Strains
journal, January 2006
- Nallapareddy, S. R.; Singh, K. V.; Murray, B. E.
- Applied and Environmental Microbiology, Vol. 72, Issue 1
High- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening
journal, December 1993
- O'Sullivan, Daniel J.; Klaenhammer, Todd R.
- Gene, Vol. 137, Issue 2, p. 227-231
Use of alkaline phosphatase fusions to study protein secretion in Bacillus subtilis.
journal, April 1991
- Payne, M. S.; Jackson, E. N.
- Journal of Bacteriology, Vol. 173, Issue 7
Plasmid vectors for Gram-positive bacteria switching from high to low copy number
journal, January 1996
- Renault, Pierre; Corthier, Gerard; Goupil, Nathalie
- Gene, Vol. 183, Issue 1-2, p. 175-182
Sequences of versatile broad-host-range vectors of the RK2 family
journal, July 2003
- Scott, Heather N.; Laible, Philip D.; Hanson, Deborah K.
- Plasmid, Vol. 50, Issue 1, p. 74-79
Identification, Cloning, and Characterization of a Lactococcus lactis Branched-Chain -Keto Acid Decarboxylase Involved in Flavor Formation
journal, January 2005
- Smit, B. A.; van Hylckama Vlieg, J. E. T.; Engels, W. J. M.
- Applied and Environmental Microbiology, Vol. 71, Issue 1
Proliferation and metabolic significance of peroxisomes in Candida boidinii during growth on d-alanine or oleic acid as the sole carbon source
journal, April 1990
- Sulter, G. J.; Waterham, H. R.; Goodman, J. M.
- Archives of Microbiology, Vol. 153, Issue 5
Crystal Structure of E.coli Alcohol Dehydrogenase YqhD: Evidence of a Covalently Modified NADP Coenzyme
journal, September 2004
- Sulzenbacher, Gerlind; Alvarez, Karine; van den Heuvel, Robert H. H.
- Journal of Molecular Biology, Vol. 342, Issue 2, p. 489-502
A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.
journal, February 1985
- Tabor, S.; Richardson, C. C.
- Proceedings of the National Academy of Sciences, Vol. 82, Issue 4
Analysis of the Conjugal Transfer System of the Pheromone- Independent Highly Transferable Enterococcus Plasmid pMG1: Identification of a tra Gene (traA) Up-Regulated during Conjugation
journal, October 2002
- Tanimoto, K.; Ike, Y.
- Journal of Bacteriology, Vol. 184, Issue 20, p. 5800-5804
Construction of highly efficient E. coli expression systems containing low oxygen induced promoter and partition region
journal, February 2005
- Liu, Tao; Chen, Jing-yu; Zheng, Zhong
- Applied Microbiology and Biotechnology, Vol. 68, Issue 3
CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
journal, January 1994
- Thompson, Julie D.; Higgins, Desmond G.; Gibson, Toby J.
- Nucleic Acids Research, Vol. 22, Issue 22, p. 4673-4680
Transcriptional Analysis of Butanol Stress and Tolerance in Clostridium acetobutylicum
journal, March 2004
- Tomas, C. A.; Beamish, J.; Papoutsakis, E. T.
- Journal of Bacteriology, Vol. 186, Issue 7
The crystal structure of a bacterial Class II ketol-acid reductoisomerase: Domain conservation and evolution
journal, December 2005
- Tyagi, Rajiv; Duquerroy, Stephane; Navaza, Jorge
- Protein Science, Vol. 14, Issue 12
Functional Analysis of Three Plasmids from Lactobacillus plantarum
journal, March 2005
- van Kranenburg, R.; Golic, N.; Bongers, R.
- Applied and Environmental Microbiology, Vol. 71, Issue 3, p. 1223-1230
Isothermal in vitro amplification of DNA by a restriction enzyme/DNA polymerase system.
journal, January 1992
- Walker, G. T.; Little, M. C.; Nadeau, J. G.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 1, p. 392-396
Purification and characterization of a fusion protein of plant acetohydroxy acid synthase and acetohydroxy acid isomeroreductase
journal, May 1997
- Dumas, Renaud; Biou, Valérie; Douce, Roland
- FEBS Letters, Vol. 408, Issue 2, p. 156-160
Isolation and kinetic properties of acetohydroxy acid isomeroreductase from spinach (Spinacia oleracea) chloroplasts overexpressed in Escherichia coli
journal, December 1992
- Dumas, R.; Job, D.; Ortholand, J. Y.
- Biochemical Journal, Vol. 288, Issue 3
Mevalonate and Nonmevalonate Pathways for the Biosynthesis of Isoprene Units
journal, January 2002
- Kuzuyama, Tomohisa
- Bioscience, Biotechnology, and Biochemistry, Vol. 66, Issue 8
Fusel Alcohols Production in Beer Fermentation Processes
journal, January 1994
- García, Ana I.; García, Luis A.; Díaz, Mario
- Process Biochemistry, Vol. 29, Issue 4, p. 303-309
Guerbet condensation of methanol with n-propanol to isobutyl alcohol over heterogeneous copper chromite/Mg–Al mixed oxides catalysts
journal, October 2004
- Carlini, Carlo; Flego, Cristina; Marchionna, Mario
- Journal of Molecular Catalysis A: Chemical, Vol. 220, Issue 2, p. 215-220
Targeting, disruption, replacement, and allele rescue: Integrative DNA transformation in yeast
book, January 1991
- Rothstein, Rodney
- Guide to Yeast Genetics and Molecular Biology
Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension
journal, April 1989
- Horton, Robert M.; Hunt, Henry D.; Ho, Steffan N.
- Gene, Vol. 77, Issue 1, p. 61-68
Complete Genomic Sequence of the Filamentous Nitrogen-fixing Cyanobacterium Anabaena sp. Strain PCC 7120 (Supplement)
journal, January 2001
- Kaneko, T.
- DNA Research, Vol. 8, Issue 5
An Investigation of the Metabolism of Valine to Isobutyl Alcohol in Saccharomyces cerevisiae
journal, October 1998
- Dickinson, J. Richard; Harrison, Scott J.; Hewlins, Michael J. E.
- Journal of Biological Chemistry, Vol. 273, Issue 40, p. 25751-25756
Formation of ethanol and higher alcohols by immobilized zymomonas mobilis in continuous culture
journal, January 1991
- Oaxaca, V. A.; Jones, L. P.
- Acta Biotechnologica, Vol. 11, Issue 6
Switch of coenzyme specificity of p -hydroxybenzoate hydroxylase 1 1Edited by A. R. Fersht
journal, September 1999
- Eppink, Michel H. M.; Overkamp, Karin M.; Schreuder, Herman A.
- Journal of Molecular Biology, Vol. 292, Issue 1
Switch of Coenzyme Specificity of Mouse Lung Carbonyl Reductase by Substitution of Threonine 38 with Aspartic Acid
journal, January 1997
- Nakanishi, Masayuki; Matsuura, Kazuya; Kaibe, Hiroyuki
- Journal of Biological Chemistry, Vol. 272, Issue 4
Identifying determinants of NADPH specificity in Baeyer-Villiger monooxygenases: NADPH specificity in BVMOs
journal, May 2004
- Kamerbeek, Nanne M.; Fraaije, Marco W.; Janssen, Dick B.
- European Journal of Biochemistry, Vol. 271, Issue 11
Towards a new interaction enzyme:coenzyme
journal, April 2005
- Martínez-Júlvez, Marta; Tejero, Jesús; R. Peregrina, José
- Biophysical Chemistry, Vol. 115, Issue 2-3, p. 219-224
Reversal of the Nucleotide Specificity of Ketol Acid Reductoisomerase by Site-Directed Mutagenesis Identifies the NADPH Binding Site1
journal, February 1997
- Rane, Madhavi J.; Calvo, K. C.
- Archives of Biochemistry and Biophysics, Vol. 338, Issue 1
Crystal Structure of Class I Acetohydroxy Acid Isomeroreductase from Pseudomonas aeruginosa
journal, April 2003
- Ahn, Hyung Jun; Eom, Su Jung; Yoon, Hye-Jin
- Journal of Molecular Biology, Vol. 328, Issue 2, p. 505-515
Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5
journal, June 2005
- Paulsen, Ian T.; Press, Caroline M.; Ravel, Jacques
- Nature Biotechnology, Vol. 23, Issue 7
NADP-Dependent enzymes. I: Conserved stereochemistry of cofactor binding
journal, May 1997
- Carugo, Oliviero; Argos, Patrick
- Proteins: Structure, Function, and Genetics, Vol. 28, Issue 1, p. 10-28
Enzymology, Structure, and Dynamics of Acetohydroxy Acid Isomeroreductase
journal, May 2001
- Dumas, Renaud; Biou, Valérie; Halgand, Frédéric
- Accounts of Chemical Research, Vol. 34, Issue 5
Modification of the Nucleotide Cofactor-binding Site of Cytochrome P-450 Reductase to Enhance Turnover with NADH in Vivo
journal, October 2002
- Elmore, C. Lee; Porter, Todd D.
- Journal of Biological Chemistry, Vol. 277, Issue 50
The X-ray structure of Brassica napus β-keto acyl carrier protein reductase and its implications for substrate binding and catalysis
journal, April 2000
- Fisher, Martin; Kroon, Johan T. M.; Martindale, Wayne
- Structure, Vol. 8, Issue 4, p. 339-347
Computational design of Candida boidinii xylose reductase for altered cofactor specificity
journal, October 2009
- Khoury, George A.; Fazelinia, Hossein; Chin, Jonathan W.
- Protein Science, Vol. 18, Issue 10
Characterization of 1-Deoxy-d-xylulose 5-Phosphate Reductoisomerase, an Enzyme Involved in Isopentenyl Diphosphate Biosynthesis, and Identification of Its Catalytic Amino Acid Residues
journal, April 2000
- Kuzuyama, Tomohisa; Takahashi, Shunji; Takagi, Motoki
- Journal of Biological Chemistry, Vol. 275, Issue 26
Probing the Determinants of Coenzyme Specificity in Ferredoxin-NADP + Reductase by Site-directed Mutagenesis
journal, January 2001
- Medina, Milagros; Luquita, Alejandra; Tejero, Jesús
- Journal of Biological Chemistry, Vol. 276, Issue 15
General approach to reversing ketol-acid reductoisomerase cofactor dependence from NADPH to NADH
journal, June 2013
- Brinkmann-Chen, S.; Flock, T.; Cahn, J. K. B.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 27, p. 10946-10951
Chemical and biological evolution of a nucleotide-binding protein
journal, July 1974
- Rossmann, Michael G.; Moras, Dino; Olsen, Kenneth W.
- Nature, Vol. 250, Issue 5463
Nucleotide sequence and characterization of a cDNA encoding the acetohydroxy acid isomeroreductase from Arabidopsis thaliana
journal, February 1993
- Curien, Gilles; Dumas, Renaud; Douce, Roland
- Plant Molecular Biology, Vol. 21, Issue 4
Purification and characterization of acetohydroxyacid reductoisomerase from spinach chloroplasts
journal, September 1989
- Dumas, R.; Joyard, J.; Douce, R.
- Biochemical Journal, Vol. 262, Issue 3
Ketol-Acid Reductoisomerase from Barley (Hordeum vulgare) (Purification, Properties, and Specific Inhibition)
journal, November 1993
- Durner, J.; Knorzer, O. C.; Boger, P.
- Plant Physiology, Vol. 103, Issue 3
A single amino acid substitution in lactate dehydrogenase improves the catalytic efficiency with an alternative coenzyme
journal, January 1990
- Feeney, Raymond; Clarke, Anthony R.; Holbrook, J. John
- Biochemical and Biophysical Research Communications, Vol. 166, Issue 2, p. 667-672
Identification and sequence determination of the acetohydroxy acid isomeroreductase gene from Brevibacterium flavum MJ233
journal, January 1993
- Inui, Masayuki; Vertégs, Alain A.; Kobayashi, Miki
- DNA Sequence, Vol. 4, Issue 2
Site-Directed Mutagenesis of a Serine Residue in Cinnamyl Alcohol Dehydrogenase, a Plant NADPH-Dependent Dehydrogenase, Affects the Specificity for the Coenzyme
journal, September 1995
- Lauvergeat, V.; Kennedy, K.; Feuillet, C.
- Biochemistry, Vol. 34, Issue 38
Engineering Escherichia coli to see light
journal, November 2005
- Levskaya, Anselm; Chevalier, Aaron A.; Tabor, Jeffrey J.
- Nature, Vol. 438, Issue 7067
Engineering of Pyridine Nucleotide Specificity of Nitrate Reductase: Mutagenesis of Recombinant CytochromebReductase Fragment ofNeurospora crassaNADPH:Nitrate Reductase
journal, October 1998
- Shiraishi, Naomasa; Croy, Cynthia; Kaur, John
- Archives of Biochemistry and Biophysics, Vol. 358, Issue 1
Probing the mechanism of the bifunctional enzyme ketol-acid reductoisomerase by site-directed mutagenesis of the active site: Mutagenesis of the active site of E. coli KARI
journal, December 2004
- Tyagi, Rajiv; Lee, Yu-Ting; Guddat, Luke W.
- FEBS Journal, Vol. 272, Issue 2
Change of Nucleotide Specificity and Enhancement of Catalytic Efficiency in Single Point Mutants of Vibrio harveyi Aldehyde Dehydrogenase †
journal, August 1999
- Zhang, Lei; Ahvazi, Bijan; Szittner, Rose
- Biochemistry, Vol. 38, Issue 35
Basic local alignment search tool
journal, October 1990
- Altschul, Stephen F.; Gish, Warren; Miller, Webb
- Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410
Environmental Stress Response in Wine Lactic Acid Bacteria: Beyond Bacillus subtilis
journal, January 2006
- Spano, G.; Massa, S.
- Critical Reviews in Microbiology, Vol. 32, Issue 2
Branched-chain amino acid biosynthesis genes in Lactococcus lactis subsp. lactis.
journal, October 1992
- Godon, J. J.; Chopin, M. C.; Ehrlich, S. D.
- Journal of Bacteriology, Vol. 174, Issue 20, p. 6580-6589
CLUSTAL V: improved software for multiple sequence alignment
journal, January 1992
- Higgins, Desmond G.; Bleasby, Alan J.; Fuchs, Rainer
- Bioinformatics, Vol. 8, Issue 2
Fast and sensitive multiple sequence alignments on a microcomputer
journal, April 1989
- Higgins, Desmond G.; Sharp, Paul M.
- Bioinformatics, Vol. 5, Issue 2, p. 151-153
DNA sequences at the ends of transposon Tn5 required for transposition
journal, July 1983
- Johnson, Reid C.; Reznikoff, William S.
- Nature, Vol. 304, Issue 5923
A synthetic promoter library for constitutive gene expression in Lactobacillus plantarum
journal, April 2006
- Rud, I.
- Microbiology, Vol. 152, Issue 4, p. 1011-1019
Use of the Escherichia coli lac repressor and operator to control gene expression in Bacillus subtilis.
journal, January 1984
- Yansura, D. G.; Henner, D. J.
- Proceedings of the National Academy of Sciences, Vol. 81, Issue 2
Regulation of groE expression in Bacillus subtilis: the involvement of the sigma A-like promoter and the roles of the inverted repeat sequence (CIRCE).
journal, October 1995
- Yuan, G.; Wong, S. L.
- Journal of Bacteriology, Vol. 177, Issue 19
Genetically engineered Saccharomyces cerevisiae for kinetic resolution of racemic bicyclo[3.3.1]nonane-2,6-dione
journal, October 2008
- Carlquist, Magnus; Wallentin, Carl-Johan; Wärnmark, Kenneth
- Tetrahedron: Asymmetry, Vol. 19, Issue 19
Decreasing acetic acid accumulation by a glycerol overproducing strain ofSaccharomyces cerevisiae by deleting theALD6 aldehyde dehydrogenase gene
journal, January 2002
- Eglinton, Jeffrey M.; Heinrich, Anthony J.; Pollnitz, Alan P.
- Yeast, Vol. 19, Issue 4
Characterization of short-chain dehydrogenase/reductase homologues of Escherichia coli (YdfG) and Saccharomyces cerevisiae (YMR226C)
journal, January 2003
- Fujisawa, Hisae; Nagata, Shinji; Misono, Haruo
- Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1645, Issue 1, p. 89-94
Strain engineering for stereoselective bioreduction of dicarbonyl compounds by yeast reductases
journal, April 2005
- Johanson, T.; Katz, M.; Gorwagrauslund, M.
- FEMS Yeast Research, Vol. 5, Issue 6-7, p. 513-525
Metabolic Engineering of Glycerol Production in Saccharomyces cerevisiae
journal, June 2002
- Overkamp, K. M.; Bakker, B. M.; Kotter, P.
- Applied and Environmental Microbiology, Vol. 68, Issue 6
Enzymatic ketone reduction: mapping the substrate profile of a short-chain alcohol dehydrogenase (YMR226c) from Saccharomyces cerevisiae
journal, August 2007
- Yang, Yan; Zhu, Dunming; Piegat, Timothy J.
- Tetrahedron: Asymmetry, Vol. 18, Issue 15, p. 1799-1803
Efficient anaerobic whole cell stereoselective bioreduction with recombinant saccharomyces cerevisiae : Stereoselective Bioreduction with
journal, September 2003
- Katz, Michael; Frejd, Torbjörn; Hahn-Hägerdal, Bärbel
- Biotechnology and Bioengineering, Vol. 84, Issue 5
The 'petite-negative' yeast Kluyveromyces lactis has a single gene expressing pyruvate decarboxylase activity
journal, January 1996
- Bianchi, Michele M.; Tizzani, Lorenza; Destruelle, Monika
- Molecular Microbiology, Vol. 19, Issue 1
Characterisation of PDC2, a gene necessary for high level expression of pyruvate decarboxylase structural genes in Saccharomyces cerevlsiae
journal, December 1993
- Hohmann, Stefan
- MGG Molecular & General Genetics, Vol. 241-241, Issue 5-6
Functional analysis of the ALD gene family of Saccharomyces cerevisiae during anaerobic growth on glucose: the NADP+-dependent Ald6p and Ald5p isoforms play a major role in acetate formation
journal, July 2004
- Saint-Prix, F.
- Microbiology, Vol. 150, Issue 7
A proposal for nomenclature of aldehyde dehydrogenases in Saccharomyces cerevisiae and characterization of the stress-inducible ALD2 and ALD3 genes
journal, July 1999
- Navarro-Aviño, Juan P.; Prasad, Rajendra; Miralles, Vicente J.
- Yeast, Vol. 15, Issue 10A, p. 829-842
Reduced pyruvate decarboxylase and increased glycerol-3-phosphate dehydrogenase [NAD+] levels enhance glycerol production in Saccharomyces cerevisiae
journal, October 1996
- Nevoigt, Elke; Stahl, Ulf
- Yeast, Vol. 12, Issue 13, p. 1331-1337
Pyruvate decarboxylase: An indispensable enzyme for growth of Saccharomyces cerevisiae on glucose
journal, March 1996
- Flikweert, Marcel T.; van der Zanden, Linda; Janssen, Wouter M. Th. M.
- Yeast, Vol. 12, Issue 3, p. 247-257
Preparation and Preservation of Freeze-dried Cells of Acetic Acid Bacteria with Aldehyde Oxidase Activity
journal, January 1998
- Nomura, Yukihiro; Yamamoto, Masanori; Matsushita, Kazunobu
- Bioscience, Biotechnology, and Biochemistry, Vol. 62, Issue 6
Secoiridoid and flavonoid glycosides from Gonocaryum calleryanum
journal, May 1995
- Kaneko, Tetsuo; Sakamoto, Mayumi; Ohtani, Kazuhiro
- Phytochemistry, Vol. 39, Issue 1, p. 115-120
Stoichiometry and compartmentation of NADH metabolism in Saccharomyces cerevisiae
journal, January 2001
- Bakker, Barbara M.; Overkamp, Karin M.; van Maris, Antonius J. A.
- FEMS Microbiology Reviews, Vol. 25, Issue 1, p. 15-37