Global to push GA events into
skip to main content

Title: High-stringency screening of target-binding partners using a microfluidic device

The invention provides a method of screening a library of candidate agents by contacting the library with a target in a reaction mixture under a condition of high stringency, wherein the target includes a tag that responds to a controllable force applied to the tag, and passing the members of the library through a microfluidic device in a manner that exposes the library members to the controllable force, thereby displacing members of the library that are bound to the target relative to their unbound counterparts. Kits and systems for use with the methods of the invention are also provided.
Inventors:
; ;
Issue Date:
OSTI Identifier:
1227363
Assignee:
The Regents of the University of California OAK
Patent Number(s):
9,200,382
Application Number:
12/286,965
Contract Number:
W-7405-ENG-48
Resource Relation:
Patent File Date: 2008 Oct 03
Research Org:
The Regents of the University of California , Oakland, CA (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Other works cited in this record:

Automated selection of anti-Protein aptamers
journal, October 2001

A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment
journal, December 2003
  • Daniels, D. A.; Chen, H.; Hicke, B. J.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 26, p. 15416-15421
  • DOI: 10.1073/pnas.2136683100

Combination of amplification and post-amplification strategies to improve optical DNA sensing
journal, December 2003
  • Giakoumaki, Elsa; Minunni, Maria; Tombelli, Sara
  • Biosensors and Bioelectronics, Vol. 19, Issue 4, p. 337-344
  • DOI: 10.1016/S0956-5663(03)00193-3

Diagnostic potential of PhotoSELEX-evolved ssDNA aptamers
journal, August 2000
  • Golden, Mace C.; Collins, Brian D.; Willis, Michael C.
  • Journal of Biotechnology, Vol. 81, Issue 2-3, p. 167-178
  • DOI: 10.1016/S0168-1656(00)00290-X

Marker-specific sorting of rare cells using dielectrophoresis
journal, October 2005
  • Hu, X.; Bessette, P. H.; Qian, J.
  • Proceedings of the National Academy of Sciences, Vol. 102, Issue 44, p. 15757-15761
  • DOI: 10.1073/pnas.0507719102

A mathematical analysis of SELEX
journal, February 2007

In vitro selection of DNA aptamers binding ethanolamine
journal, December 2005
  • Mann, Doerthe; Reinemann, Christine; Stoltenburg, Regina
  • Biochemical and Biophysical Research Communications, Vol. 338, Issue 4, p. 1928-1934
  • DOI: 10.1016/j.bbrc.2005.10.172

Selection of RNA aptamers against human influenza virus hemagglutinin using surface plasmon resonance
journal, July 2005
  • Misono, Tomoko S.; Kumar, Penmetcha K. R.
  • Analytical Biochemistry, Vol. 342, Issue 2, p. 312-317
  • DOI: 10.1016/j.ab.2005.04.013

Diffusion coefficient of DNA molecules during free solution electrophoresis
journal, July 2001

In Vitro Selection of Structure-Switching Signaling Aptamers
journal, February 2005
  • Nutiu, Razvan; Li, Yingfu
  • Angewandte Chemie International Edition, Vol. 44, Issue 7, p. 1061-1065
  • DOI: 10.1002/anie.200461848

Liquid chromatography, electrochromatography and capillary electrophoresis applications of DNA and RNA aptamers
journal, June 2006
  • Ravelet, Corinne; Grosset, Catherine; Peyrin, Eric
  • Journal of Chromatography A, Vol. 1117, Issue 1, p. 1-10
  • DOI: 10.1016/j.chroma.2006.03.101

Aptamers-based assays for diagnostics, environmental and food analysis
journal, June 2007

Electronic Aptamer-Based Sensors
journal, August 2007
  • Willner, Itamar; Zayats, Maya
  • Angewandte Chemie International Edition, Vol. 46, Issue 34, p. 6408-6418
  • DOI: 10.1002/anie.200604524

Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor
journal, August 2005
  • Xiao, Yi; Lubin, Arica A.; Heeger, Alan J.
  • Angewandte Chemie International Edition, Vol. 44, Issue 34, p. 5456-5459
  • DOI: 10.1002/anie.200500989

mRNA display: Diversity matters during in vitro selection
journal, April 2001
  • Gold, L.
  • Proceedings of the National Academy of Sciences, Vol. 98, Issue 9, p. 4825-4826
  • DOI: 10.1073/pnas.091101698

In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
book, March 2010

Theoretical Principles of In Vitro Selection Using Combinatorial Nucleic Acid Libraries
book, February 2000
  • Vant-Hull, Barry; Gold, Larry; Zichi, Dominic A.
  • Current Protocols in Nucleic Acid Chemistry, p. 9.1:9.1.1–9.1.16
  • DOI: 10.1002/0471142700.nc0901s00

Similar records in DOepatents and OSTI.GOV collections: