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Title: High-stringency screening of target-binding partners using a microfluidic device

Abstract

The invention provides a method of screening a library of candidate agents by contacting the library with a target in a reaction mixture under a condition of high stringency, wherein the target includes a tag that responds to a controllable force applied to the tag, and passing the members of the library through a microfluidic device in a manner that exposes the library members to the controllable force, thereby displacing members of the library that are bound to the target relative to their unbound counterparts. Kits and systems for use with the methods of the invention are also provided.

Inventors:
; ;
Issue Date:
Research Org.:
The Regents of the University of California , Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1227363
Patent Number(s):
9,200,382
Application Number:
12/286,965
Assignee:
The Regents of the University of California
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 Oct 03
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Soh, Hyongsok, Lou, Xinhui, and Lagally, Eric. High-stringency screening of target-binding partners using a microfluidic device. United States: N. p., 2015. Web.
Soh, Hyongsok, Lou, Xinhui, & Lagally, Eric. High-stringency screening of target-binding partners using a microfluidic device. United States.
Soh, Hyongsok, Lou, Xinhui, and Lagally, Eric. Tue . "High-stringency screening of target-binding partners using a microfluidic device". United States. https://www.osti.gov/servlets/purl/1227363.
@article{osti_1227363,
title = {High-stringency screening of target-binding partners using a microfluidic device},
author = {Soh, Hyongsok and Lou, Xinhui and Lagally, Eric},
abstractNote = {The invention provides a method of screening a library of candidate agents by contacting the library with a target in a reaction mixture under a condition of high stringency, wherein the target includes a tag that responds to a controllable force applied to the tag, and passing the members of the library through a microfluidic device in a manner that exposes the library members to the controllable force, thereby displacing members of the library that are bound to the target relative to their unbound counterparts. Kits and systems for use with the methods of the invention are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {12}
}

Patent:

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