skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: High-stringency screening of target-binding partners using a microfluidic device

Abstract

The invention provides a method of screening a library of candidate agents by contacting the library with a target in a reaction mixture under a condition of high stringency, wherein the target includes a tag that responds to a controllable force applied to the tag, and passing the members of the library through a microfluidic device in a manner that exposes the library members to the controllable force, thereby displacing members of the library that are bound to the target relative to their unbound counterparts. Kits and systems for use with the methods of the invention are also provided.

Inventors:
; ;
Issue Date:
Research Org.:
The Regents of the University of California , Oakland, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1227363
Patent Number(s):
9200382
Application Number:
12/286,965
Assignee:
The Regents of the University of California
Patent Classifications (CPCs):
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
DOE Contract Number:  
W-7405-ENG-48
Resource Type:
Patent
Resource Relation:
Patent File Date: 2008 Oct 03
Country of Publication:
United States
Language:
English
Subject:
47 OTHER INSTRUMENTATION

Citation Formats

Soh, Hyongsok, Lou, Xinhui, and Lagally, Eric. High-stringency screening of target-binding partners using a microfluidic device. United States: N. p., 2015. Web.
Soh, Hyongsok, Lou, Xinhui, & Lagally, Eric. High-stringency screening of target-binding partners using a microfluidic device. United States.
Soh, Hyongsok, Lou, Xinhui, and Lagally, Eric. Tue . "High-stringency screening of target-binding partners using a microfluidic device". United States. https://www.osti.gov/servlets/purl/1227363.
@article{osti_1227363,
title = {High-stringency screening of target-binding partners using a microfluidic device},
author = {Soh, Hyongsok and Lou, Xinhui and Lagally, Eric},
abstractNote = {The invention provides a method of screening a library of candidate agents by contacting the library with a target in a reaction mixture under a condition of high stringency, wherein the target includes a tag that responds to a controllable force applied to the tag, and passing the members of the library through a microfluidic device in a manner that exposes the library members to the controllable force, thereby displacing members of the library that are bound to the target relative to their unbound counterparts. Kits and systems for use with the methods of the invention are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2015},
month = {12}
}

Patent:

Save / Share:

Works referenced in this record:

Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures: A Universal Tool for Development of Aptamers
journal, March 2005


Non-SELEX Selection of Aptamers
journal, February 2006


Systematic Evolution of a DNA Aptamer Binding to Rat Brain Tumor Microvessels: SELECTIVE TARGETING OF ENDOTHELIAL REGULATORY PROTEIN PIGPEN
journal, February 2001


Selection of single-stranded DNA molecules that bind and inhibit human thrombin
journal, February 1992


SELEX: Just another separation?
journal, January 2005


Automated selection of anti-Protein aptamers
journal, October 2001


Automated RNA Selection
journal, December 1998


A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment
journal, December 2003


Selection of Smart Aptamers by Equilibrium Capillary Electrophoresis of Equilibrium Mixtures (ECEEM)
journal, August 2005


In vitro selection of RNA molecules that bind specific ligands
journal, August 1990


Combination of amplification and post-amplification strategies to improve optical DNA sensing
journal, December 2003


Diagnostic potential of PhotoSELEX-evolved ssDNA aptamers
journal, August 2000


Methods developed for SELEX
journal, October 2006


Marker-specific sorting of rare cells using dielectrophoresis
journal, October 2005


Continuous microfluidic immunomagnetic cell separation
journal, November 2004


A planar polymer microfluidic electrocapture device for beads immobilization
journal, August 2006


Aptamer-Based Sensor Arrays for the Detection and Quantitation of Proteins
journal, July 2004


A mathematical analysis of SELEX
journal, February 2007


In vitro selection of DNA aptamers binding ethanolamine
journal, December 2005


In Vitro Selection of High-Affinity DNA Ligands for Human IgE Using Capillary Electrophoresis
journal, September 2004


In Vitro Evolution of Functional DNA Using Capillary Electrophoresis
journal, January 2004


Selection of RNA aptamers against human influenza virus hemagglutinin using surface plasmon resonance
journal, July 2005


One-step selection of Vaccinia virus-binding DNA aptamers by MonoLEX
journal, January 2007


Diffusion coefficient of DNA molecules during free solution electrophoresis
journal, July 2001


In Vitro Selection of Structure-Switching Signaling Aptamers
journal, February 2005


Microfluidic Protein Detection through Genetically Engineered Bacterial Cells
journal, December 2006


Isolation of virus-neutralizing RNAs from a large pool of random sequences.
journal, December 1995


Liquid chromatography, electrochromatography and capillary electrophoresis applications of DNA and RNA aptamers
journal, June 2006


Complex Target SELEX
journal, January 2008


Cell-Specific Aptamer Probes for Membrane Protein Elucidation in Cancer Cells
journal, May 2008


FluMag-SELEX as an advantageous method for DNA aptamer selection
journal, July 2005


Single microbead SELEX for efficient ssDNA aptamer generation against botulinum neurotoxin
journal, January 2008


Aptamers-based assays for diagnostics, environmental and food analysis
journal, June 2007


DNA and RNA Aptamers: From Tools for Basic Research Towards Therapeutic Applications
journal, September 2006


Selection of RNA Aptamers that Bind Specifically to the NS3 Protease of Hepatitis C Virus
journal, August 1997


Electronic Aptamer-Based Sensors
journal, August 2007


Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor
journal, August 2005


Selection of Smart Aptamers by Methods of Kinetic Capillary Electrophoresis
journal, May 2006


mRNA display: Diversity matters during in vitro selection
journal, April 2001


In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
book, March 2010


Capillary Electrophoresis-SELEX Selection of Aptamers with Affinity for HIV-1 Reverse Transcriptase
journal, October 2005


Theoretical Principles of In Vitro Selection Using Combinatorial Nucleic Acid Libraries
book, February 2000