High-stringency screening of target-binding partners using a microfluidic device
Abstract
The invention provides a method of screening a library of candidate agents by contacting the library with a target in a reaction mixture under a condition of high stringency, wherein the target includes a tag that responds to a controllable force applied to the tag, and passing the members of the library through a microfluidic device in a manner that exposes the library members to the controllable force, thereby displacing members of the library that are bound to the target relative to their unbound counterparts. Kits and systems for use with the methods of the invention are also provided.
- Inventors:
- Issue Date:
- Research Org.:
- The Regents of the University of California , Oakland, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1227363
- Patent Number(s):
- 9200382
- Application Number:
- 12/286,965
- Assignee:
- The Regents of the University of California
- Patent Classifications (CPCs):
-
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY
B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- DOE Contract Number:
- W-7405-ENG-48
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 2008 Oct 03
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 47 OTHER INSTRUMENTATION
Citation Formats
Soh, Hyongsok, Lou, Xinhui, and Lagally, Eric. High-stringency screening of target-binding partners using a microfluidic device. United States: N. p., 2015.
Web.
Soh, Hyongsok, Lou, Xinhui, & Lagally, Eric. High-stringency screening of target-binding partners using a microfluidic device. United States.
Soh, Hyongsok, Lou, Xinhui, and Lagally, Eric. Tue .
"High-stringency screening of target-binding partners using a microfluidic device". United States. https://www.osti.gov/servlets/purl/1227363.
@article{osti_1227363,
title = {High-stringency screening of target-binding partners using a microfluidic device},
author = {Soh, Hyongsok and Lou, Xinhui and Lagally, Eric},
abstractNote = {The invention provides a method of screening a library of candidate agents by contacting the library with a target in a reaction mixture under a condition of high stringency, wherein the target includes a tag that responds to a controllable force applied to the tag, and passing the members of the library through a microfluidic device in a manner that exposes the library members to the controllable force, thereby displacing members of the library that are bound to the target relative to their unbound counterparts. Kits and systems for use with the methods of the invention are also provided.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Dec 01 00:00:00 EST 2015},
month = {Tue Dec 01 00:00:00 EST 2015}
}
Works referenced in this record:
Methods for identifying nucleic acid ligands
patent, December 1993
- Gold, Larry; Tuerk, Craig
- US Patent Document 5,270,163
Microfluidic devices for high gradient magnetic separation
patent-application, January 2004
- Ward, Michael Dennis; Grodzinski, Piotr; Liu, Robin Hui
- US Patent Application 10/202462; 20040018611
Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures: A Universal Tool for Development of Aptamers
journal, March 2005
- Berezovski, Maxim; Drabovich, Andrei; Krylova, Svetlana M.
- Journal of the American Chemical Society, Vol. 127, Issue 9
Non-SELEX Selection of Aptamers
journal, February 2006
- Berezovski, Maxim; Musheev, Michael; Drabovich, Andrei
- Journal of the American Chemical Society, Vol. 128, Issue 5
Systematic Evolution of a DNA Aptamer Binding to Rat Brain Tumor Microvessels: SELECTIVE TARGETING OF ENDOTHELIAL REGULATORY PROTEIN PIGPEN
journal, February 2001
- Blank, Michael; Weinschenk, Toni; Priemer, Martin
- Journal of Biological Chemistry, Vol. 276, Issue 19
Selection of single-stranded DNA molecules that bind and inhibit human thrombin
journal, February 1992
- Bock, Louis C.; Griffin, Linda C.; Latham, John A.
- Nature, Vol. 355, Issue 6360
SELEX: Just another separation?
journal, January 2005
- Bowser, Michael T.
- The Analyst, Vol. 130, Issue 2
Automated selection of anti-Protein aptamers
journal, October 2001
- Cox, J. Colin; Ellington, Andrew D.
- Bioorganic & Medicinal Chemistry, Vol. 9, Issue 10, p. 2525-2531
Automated selection of aptamers against protein targets translated in vitro: from gene to aptamer
journal, October 2002
- Cox, J. C.
- Nucleic Acids Research, Vol. 30, Issue 20
Automated RNA Selection
journal, December 1998
- Cox, J. C.; Rudolph, P.; Ellington, A. D.
- Biotechnology Progress, Vol. 14, Issue 6
A tenascin-C aptamer identified by tumor cell SELEX: Systematic evolution of ligands by exponential enrichment
journal, December 2003
- Daniels, D. A.; Chen, H.; Hicke, B. J.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 26, p. 15416-15421
Selection of Smart Aptamers by Equilibrium Capillary Electrophoresis of Equilibrium Mixtures (ECEEM)
journal, August 2005
- Drabovich, Andrei; Berezovski, Maxim; Krylov, Sergey N.
- Journal of the American Chemical Society, Vol. 127, Issue 32
In vitro selection of RNA molecules that bind specific ligands
journal, August 1990
- Ellington, Andrew D.; Szostak, Jack W.
- Nature, Vol. 346, Issue 6287
Combination of amplification and post-amplification strategies to improve optical DNA sensing
journal, December 2003
- Giakoumaki, Elsa; Minunni, Maria; Tombelli, Sara
- Biosensors and Bioelectronics, Vol. 19, Issue 4, p. 337-344
Diagnostic potential of PhotoSELEX-evolved ssDNA aptamers
journal, August 2000
- Golden, Mace C.; Collins, Brian D.; Willis, Michael C.
- Journal of Biotechnology, Vol. 81, Issue 2-3, p. 167-178
Methods developed for SELEX
journal, October 2006
- Gopinath, Subash Chandra Bose
- Analytical and Bioanalytical Chemistry, Vol. 387, Issue 1
An RNA aptamer that distinguishes between closely related human influenza viruses and inhibits haemagglutinin-mediated membrane fusion
journal, March 2006
- Gopinath, S. C. B.
- Journal of General Virology, Vol. 87, Issue 3
Marker-specific sorting of rare cells using dielectrophoresis
journal, October 2005
- Hu, X.; Bessette, P. H.; Qian, J.
- Proceedings of the National Academy of Sciences, Vol. 102, Issue 44, p. 15757-15761
Continuous microfluidic immunomagnetic cell separation
journal, November 2004
- Inglis, David W.; Riehn, R.; Austin, R. H.
- Applied Physics Letters, Vol. 85, Issue 21
A planar polymer microfluidic electrocapture device for beads immobilization
journal, August 2006
- Jönsson, Mats; Lindberg, Ulf
- Journal of Micromechanics and Microengineering, Vol. 16, Issue 10
Aptamer-Based Sensor Arrays for the Detection and Quantitation of Proteins
journal, July 2004
- Kirby, Romy; Cho, Eun Jeong; Gehrke, Brian
- Analytical Chemistry, Vol. 76, Issue 14
A mathematical analysis of SELEX
journal, February 2007
- Levine, Howard A.; Nilsen-Hamilton, Marit
- Computational Biology and Chemistry, Vol. 31, Issue 1, p. 11-35
Dynamic arraying of microbeads for bioassays in microfluidic channels
journal, May 2005
- Lilliehorn, Tobias; Nilsson, Mikael; Simu, Urban
- Sensors and Actuators B: Chemical, Vol. 106, Issue 2, p. 851-858
In vitro selection of DNA aptamers binding ethanolamine
journal, December 2005
- Mann, Doerthe; Reinemann, Christine; Stoltenburg, Regina
- Biochemical and Biophysical Research Communications, Vol. 338, Issue 4, p. 1928-1934
In Vitro Selection of High-Affinity DNA Ligands for Human IgE Using Capillary Electrophoresis
journal, September 2004
- Mendonsa, Shaun D.; Bowser, Michael T.
- Analytical Chemistry, Vol. 76, Issue 18
In Vitro Evolution of Functional DNA Using Capillary Electrophoresis
journal, January 2004
- Mendonsa, Shaun D.; Bowser, Michael T.
- Journal of the American Chemical Society, Vol. 126, Issue 1
Selection of RNA aptamers against human influenza virus hemagglutinin using surface plasmon resonance
journal, July 2005
- Misono, Tomoko S.; Kumar, Penmetcha K. R.
- Analytical Biochemistry, Vol. 342, Issue 2, p. 312-317
An improved method for the in vitro evolution of aptamers and applications in protein detection and purification
journal, September 2003
- Murphy, M. B.
- Nucleic Acids Research, Vol. 31, Issue 18
One-step selection of Vaccinia virus-binding DNA aptamers by MonoLEX
journal, January 2007
- Nitsche, Andreas; Kurth, Andreas; Dunkhorst, Anna
- BMC Biotechnology, Vol. 7, Issue 1
Diffusion coefficient of DNA molecules during free solution electrophoresis
journal, July 2001
- Nkodo, Axel E.; Garnier, Jean M.; Tinland, Bernard
- ELECTROPHORESIS, Vol. 22, Issue 12, p. 2424-2432
In Vitro Selection of Structure-Switching Signaling Aptamers
journal, February 2005
- Nutiu, Razvan; Li, Yingfu
- Angewandte Chemie International Edition, Vol. 44, Issue 7, p. 1061-1065
Microfluidic Protein Detection through Genetically Engineered Bacterial Cells
journal, December 2006
- Oh, Sang-Hyun; Lee, Sang-Ho; Kenrick, Sophia A.
- Journal of Proteome Research, Vol. 5, Issue 12
Isolation of virus-neutralizing RNAs from a large pool of random sequences.
journal, December 1995
- Pan, W.; Craven, R. C.; Qiu, Q.
- Proceedings of the National Academy of Sciences, Vol. 92, Issue 25
Liquid chromatography, electrochromatography and capillary electrophoresis applications of DNA and RNA aptamers
journal, June 2006
- Ravelet, Corinne; Grosset, Catherine; Peyrin, Eric
- Journal of Chromatography A, Vol. 1117, Issue 1, p. 1-10
Complex Target SELEX
journal, January 2008
- Shamah, Steven M.; Healy, Judith M.; Cload, Sharon T.
- Accounts of Chemical Research, Vol. 41, Issue 1
Cell-Specific Aptamer Probes for Membrane Protein Elucidation in Cancer Cells
journal, May 2008
- Shangguan, Dihua; Cao, Zehui; Meng, Ling
- Journal of Proteome Research, Vol. 7, Issue 5
Aptamers Evolved from Cultured Cancer Cells Reveal Molecular Differences of Cancer Cells in Patient Samples
journal, April 2007
- Shangguan, D.; Cao, Z. C.; Li, Y.
- Clinical Chemistry, Vol. 53, Issue 6
FluMag-SELEX as an advantageous method for DNA aptamer selection
journal, July 2005
- Stoltenburg, R.; Reinemann, C.; Strehlitz, B.
- Analytical and Bioanalytical Chemistry, Vol. 383, Issue 1
Single microbead SELEX for efficient ssDNA aptamer generation against botulinum neurotoxin
journal, January 2008
- Tok, Jeffrey B. -H.; Fischer, Nicholas O.
- Chemical Communications, Issue 16
Aptamers-based assays for diagnostics, environmental and food analysis
journal, June 2007
- Tombelli, Sara; Minunni, Maria; Mascini, Marco
- Biomolecular Engineering, Vol. 24, Issue 2, p. 191-200
Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase
journal, August 1990
- Tuerk, C.; Gold, L.
- Science, Vol. 249, Issue 4968
DNA and RNA Aptamers: From Tools for Basic Research Towards Therapeutic Applications
journal, September 2006
- Ulrich, Henning; Trujillo, Cleber; Nery, Arthur
- Combinatorial Chemistry & High Throughput Screening, Vol. 9, Issue 8
Selection of RNA Aptamers that Bind Specifically to the NS3 Protease of Hepatitis C Virus
journal, August 1997
- Urvil, Petri T.; Kakiuchi, Nobuko; Zhou, De-Min
- European Journal of Biochemistry, Vol. 248, Issue 1
Electronic Aptamer-Based Sensors
journal, August 2007
- Willner, Itamar; Zayats, Maya
- Angewandte Chemie International Edition, Vol. 46, Issue 34, p. 6408-6418
Label-Free Electronic Detection of Thrombin in Blood Serum by Using an Aptamer-Based Sensor
journal, August 2005
- Xiao, Yi; Lubin, Arica A.; Heeger, Alan J.
- Angewandte Chemie International Edition, Vol. 44, Issue 34, p. 5456-5459
Selection of Smart Aptamers by Methods of Kinetic Capillary Electrophoresis
journal, May 2006
- Drabovich, Andrei P.; Berezovski, Maxim; Okhonin, Victor
- Analytical Chemistry, Vol. 78, Issue 9
mRNA display: Diversity matters during in vitro selection
journal, April 2001
- Gold, L.
- Proceedings of the National Academy of Sciences, Vol. 98, Issue 9, p. 4825-4826
In Vitro Selection of RNA Aptamers to a Protein Target by Filter Immobilization
book, March 2010
- Hall, Bradley; Arshad, Seyed; Seo, Kyunghyun
- Current Protocols in Nucleic Acid Chemistry
Capillary Electrophoresis-SELEX Selection of Aptamers with Affinity for HIV-1 Reverse Transcriptase
journal, October 2005
- Mosing, Renee K.; Mendonsa, Shaun D.; Bowser, Michael T.
- Analytical Chemistry, Vol. 77, Issue 19
Theoretical Principles of In Vitro Selection Using Combinatorial Nucleic Acid Libraries
book, February 2000
- Vant-Hull, Barry; Gold, Larry; Zichi, Dominic A.
- Current Protocols in Nucleic Acid Chemistry, p. 9.1:9.1.1–9.1.16
Nonequilibrium Capillary Electrophoresis of Equilibrium Mixtures − A Single Experiment Reveals Equilibrium and Kinetic Parameters of Protein−DNA Interactions
journal, November 2002
- Berezovski, Maxim; Krylov, Sergey N.
- Journal of the American Chemical Society, Vol. 124, Issue 46
[14] In vitro selection of RNA aptamers
book, January 2000
- Marshall, Kristin A.; Ellington, Andrew D.
- Methods in Enzymology
Short bioactive Spiegelmers to migraine-associated calcitonin gene-related peptide rapidly identified by a novel approach: Tailored-SELEX
journal, November 2003
- Vater, A.
- Nucleic Acids Research, Vol. 31, Issue 21