Title: High-stringency screening of target-binding partners using a microfluidic device

Abstract

The invention provides a method of screening a library of candidate agents by contacting the library with a target in a reaction mixture under a condition of high stringency, wherein the target includes a tag that responds to a controllable force applied to the tag, and passing the members of the library through a microfluidic device in a manner that exposes the library members to the controllable force, thereby displacing members of the library that are bound to the target relative to their unbound counterparts. Kits and systems for use with the methods of the invention are also provided.

Inventors:

Soh, Hyongsok; Lou, Xinhui; Lagally, Eric

Issue Date:

Tue Dec 01 00:00:00 EST 2015

Research Org.:

The Regents of the University of California , Oakland, CA (United States)

Sponsoring Org.:

USDOE

OSTI Identifier:

1227363

Patent Number(s):

9200382

Application Number:

12/286,965

Assignee:

The Regents of the University of California

Patent Classifications (CPCs):

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

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B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01J - CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY

B01J2219/005 - Beads
B01J2219/00563 - Magnetic means
B01J2219/00722 - Nucleotides
B01J2219/00725 - Peptides
B01J2219/085 - creating magnetic fields

B - PERFORMING OPERATIONS B01 - PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL B01L - CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE

B01L2200/0652 - Sorting or classification of particles or molecules
B01L2400/043 - magnetic forces
B01L2400/0454 - radiation pressure, optical tweezers
B01L3/502761 - {specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules

C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES

C12N15/1013 - {by using magnetic beads}
C12N15/1034 - {Isolating an individual clone by screening libraries}

C - CHEMISTRY C40 - COMBINATORIAL TECHNOLOGY C40B - COMBINATORIAL CHEMISTRY

C40B30/04 - by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
C40B70/00 - Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes

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DOE Contract Number:  

W-7405-ENG-48

Resource Type:

Patent

Resource Relation:

Patent File Date: 2008 Oct 03

Country of Publication:

United States

Language:

English

Subject:

47 OTHER INSTRUMENTATION