Compositions containing nucleosides and manganese and their uses

Title: Compositions containing nucleosides and manganese and their uses

This invention encompasses methods of preserving protein function by contacting a protein with a composition comprising one or more purine or pyrimidine nucleosides (such as e.g., adenosine or uridine) and an antioxidant (such as e.g., manganese). In addition, the invention encompasses methods of treating and/or preventing a side effect of radiation exposure and methods of preventing a side effect of radiotherapy comprising administration of a pharmaceutically effective amount of a composition comprising one or more purine or pyrimidine nucleosides (such as e.g., adenosine or uridine) and an antioxidant (such as e.g., manganese) to a subject in need thereof. The compositions may comprise D. radiodurans extracts.

Inventors:: Daly, Michael J.; Gaidamakova, Elena K.; Matrosova, Vera Y.; Levine, Rodney L.; Wehr, Nancy B.

Issue Date:: 2015-11-17

OSTI Identifier:: 1226548

Assignee:: The Henry M. Jackson Foundation for Advancement of Military Medicine, Inc. (Bethesda, MD) CHO

Patent Number(s):: 9,186,406

Application Number:: 12/673,709

Contract Number:: FG02-04ER63918

Resource Relation:: Patent File Date: 2008 Aug 18

Research Org:: The Henry M. Jackson Foundation for Advancement of Military Medicine, Inc., Bethesda, MD (United States)

Sponsoring Org:: USDOE

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES

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Other works cited in this record:

Stabilized liquid enzyme composition for glucose determination
patent, January 1990

Gawronski, Thomas
US Patent Document 4,897,346
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F4897346

Process for the production on an antiviral vaccine, particularly anti-foot and mouth disease vaccine
patent, September 1990

Lombardo, Jorge; Smolko, Eduardo; Ugalde, Rodolfo
US Patent Document 4,959,211
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F4959211

Infant formulas and nutrition products enriched with nucleosides and/or nucleotides and processes for their preparation
patent, November 1991

Gil, Angel; Morales, Daniel; Valverde, Eduardo
US Patent Document 5,066,500
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F5066500

Method and apparatus of a defined serumfree medical solution
patent, April 1995

Lindstrom, Richard; Skelnik, Debra
US Patent Document 5,407,669
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F5407669

Nutritive medium for the culture of microorganism
patent, July 1996

Jay, Corinne
US Patent Document 5,536,645
URL: http://patft.uspto.gov/netacgi/nph-Parser?Sect2=PTO1&Sect2=HITOFF&p=1&u=%2Fnetahtml%2FPTO%2Fsearch-bool.html&r=1&f=G&l=50&d=PALL&RefSrch=yes&Query=PN%2F5536645

Protein Oxidation Implicated as the Primary Determinant of Bacterial Radioresistance
journal, March 2007

Daly, Michael J.; Gaidamakova, Elena K.; Matrosova, Vera Y.
PLoS Biology, Vol. 5, Issue 4, Article No. e92
DOI: 10.1371/journal.pbio.0050092

Accumulation of Mn(II) in Deinococcus radiodurans Facilitates Gamma-Radiation Resistance
journal, November 2004

Daly, M. J.; Gaidamakova, E. K.; Matrosova, V. Y.
Science, Vol. 306, Issue 5698, p. 1025-1028
DOI: 10.1126/science.1103185

Small-Molecule Antioxidant Proteome-Shields in Deinococcus radiodurans
journal, September 2010

Daly, Michael J.; Gaidamakova, Elena K.; Matrosova, Vera Y.
PLoS ONE, Vol. 5, Issue 9, Article No. e12570
DOI: 10.1371/journal.pone.0012570

Identification of protein components and quantitative immunoassay for SEC2 in staphylococcin injection
journal, August 2009

Ding, Ding; Huang, Peng; Sun, Hongying
Journal of Pharmaceutical and Biomedical Analysis, Vol. 50, Issue 1, p. 79-85
DOI: 10.1016/j.jpba.2009.03.024

Preserving Immunogenicity of Lethally Irradiated Viral and Bacterial Vaccine Epitopes Using a Radio- Protective Mn²⁺-Peptide Complex from Deinococcus
journal, July 2012

Gaidamakova, Elena K.; Myles, Ian A.; McDaniel, Dennis P.
Cell Host & Microbe, Vol. 12, Issue 1, p. 117-124
DOI: 10.1016/j.chom.2012.05.011

Protein damage and death by radiation in Escherichia coli and Deinococcus radiodurans
journal, July 2010

Krisko, A.; Radman, M.
Proceedings of the National Academy of Sciences, Vol. 107, Issue 32, p. 14373-14377
DOI: 10.1073/pnas.1009312107

A Manganese Porphyrin Complex is a Novel Radiation Protector
journal, July 2004

Lee, Jin Hyup; Park, Jeen-Woo
Free Radical Biology and Medicine, Vol. 37, Issue 2, p. 272-283
DOI: 10.1016/j.freeradbiomed.2004.04.029

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