Global to push GA events into
skip to main content

Title: Compositions containing amino acids, phosphate and manganese and their uses

The invention provides methods of producing vaccines directed against microorganisms, with the methods comprising culturing, harvesting and/or suspending the microorganism in the presence of a radiation-protective composition and irradiating the bacteria or viruses with a dose of radiation sufficient to render the microorganism replication-deficient and/or non-infective. The radiation-protective compositions used in the methods of the present invention comprise at least one nucleoside, at least one antioxidant and at least one small peptide. The invention also provides methods of rendering bacteria in culture resistant to ionizing radiation (IR), with these methods comprising culturing the bacteria in the presence of a radiation-protective composition.
Inventors:
;
Issue Date:
OSTI Identifier:
1234646
Assignee:
The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc. (Bethesda, MD) CHO
Patent Number(s):
9,234,168
Application Number:
13/643,794
Contract Number:
FG02-04ER63918
Resource Relation:
Patent File Date: 2011 Apr 29
Research Org:
The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
60 APPLIED LIFE SCIENCES; 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.

Other works cited in this record:

Small-Molecule Antioxidant Proteome-Shields in Deinococcus radiodurans
journal, September 2010
  • Daly, Michael J.; Gaidamakova, Elena K.; Matrosova, Vera Y.
  • PLoS ONE, Vol. 5, Issue 9, Article No. e12570
  • DOI: 10.1371/journal.pone.0012570

Death by protein damage in irradiated cells
journal, January 2012

Protein tolerance to random amino acid change
journal, June 2004
  • Guo, H. H.; Choe, J.; Loeb, L. A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210
  • DOI: 10.1073/pnas.0403255101

Vaccination with Irradiated Listeria Induces Protective T Cell Immunity
journal, July 2006

Accumulation of Mn(II) in Deinococcus radiodurans Facilitates Gamma-Radiation Resistance
journal, November 2004
  • Daly, M. J.; Gaidamakova, E. K.; Matrosova, V. Y.
  • Science, Vol. 306, Issue 5698, p. 1025-1028
  • DOI: 10.1126/science.1103185

Identification of protein components and quantitative immunoassay for SEC2 in staphylococcin injection
journal, August 2009
  • Ding, Ding; Huang, Peng; Sun, Hongying
  • Journal of Pharmaceutical and Biomedical Analysis, Vol. 50, Issue 1, p. 79-85
  • DOI: 10.1016/j.jpba.2009.03.024

Preserving Immunogenicity of Lethally Irradiated Viral and Bacterial Vaccine Epitopes Using a Radio- Protective Mn2+-Peptide Complex from Deinococcus
journal, July 2012
  • Gaidamakova, Elena K.; Myles, Ian A.; McDaniel, Dennis P.
  • Cell Host & Microbe, Vol. 12, Issue 1, p. 117-124
  • DOI: 10.1016/j.chom.2012.05.011

Protein damage and death by radiation in Escherichia coli and Deinococcus radiodurans
journal, July 2010
  • Krisko, A.; Radman, M.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 32, p. 14373-14377
  • DOI: 10.1073/pnas.1009312107

A Manganese Porphyrin Complex is a Novel Radiation Protector
journal, July 2004

Potential Utility of Radioprotective Agents in the Practice of Nuclear Medicine
journal, December 2009
  • Hosseinimehr, Seyed Jalal
  • Cancer Biotherapy & Radiopharmaceuticals, Vol. 24, Issue 6, p. 723-731
  • DOI: 10.1089/cbr.2009.0635

Protein Oxidation Implicated as the Primary Determinant of Bacterial Radioresistance
journal, March 2007
  • Daly, Michael J.; Gaidamakova, Elena K.; Matrosova, Vera Y.
  • PLoS Biology, Vol. 5, Issue 4, Article No. e92
  • DOI: 10.1371/journal.pbio.0050092

Similar records in DOepatents and OSTI.GOV collections: