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Title: Genes encoding chavicol/eugenol synthase from the creosote bush Larrea tridentata

Particular aspects provide novel methods for redirecting carbon allocation in plants or cell culture from lignification to inherently more useful and tractable materials, and to facilitate the generation of, e.g., biofuels from the remaining plant ro culture biomass. Particular aspects provided novel methods for converting monolignols into allyl/propenyl phenols, and for chavicol/eugenol formation or production. Additional aspects relate to the discovery of novel chavicol/eugenol synthases that convert p-coumaryl/coniferyl alcohol esters into chavicol/eugenol, and to novel compositions (e.g., novel proteins and nucleic acids encoding same), and novel methods using same for producing or forming chavicol/eugenol and other derivatives in cell culture and/or genetically modified plants, and for re-engineering the composition of plant biomass. Particular aspects provide novel methods for generation in culture or in planta of liquid/combustible allyl/propenyl phenols, and these phenolic products are utilized for (non-ethanol) biofuel/bioenergy purposes, while the remaining plant biomass facilitates the generation of other biofuels.
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Issue Date:
OSTI Identifier:
Washington State University (Pullman, WA) OAK
Patent Number(s):
Application Number:
Contract Number:
Resource Relation:
Patent File Date: 2007 May 29
Research Org:
Washington State Univ., Pullman, WA (United States)
Sponsoring Org:
Country of Publication:
United States

Works referenced in this record:

(+)-Larreatricin hydroxylase, an enantio-specific polyphenol oxidase from the creosote bush (Larrea tridentata)
journal, September 2003
  • Cho, M.-H.; Moinuddin, S. G. A.; Helms, G. L.
  • Proceedings of the National Academy of Sciences, Vol. 100, Issue 19, p. 10641-10646
  • DOI: 10.1073/pnas.1934562100

Dirigent phenoxy radical coupling advances and challenges
journal, August 2005

Eugenol and isoeugenol, characteristic aromatic constituents of spices, are biosynthesized via reduction of a coniferyl alcohol ester
journal, June 2006
  • Koeduka, T.; Fridman, E.; Gang, D. R.
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  • DOI: 10.1073/pnas.0603732103

Plant genetic engineering to improve biomass characteristics for biofuels
journal, June 2006