skip to main content
DOE Patents title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush)

Abstract

The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac-TAG production. Additionally, oils produced by methods of the present inventions comprising genes and proteins are contemplated for use as biodiesel fuel, in polymer production and as naturally produced food oils with reduced calories.

Inventors:
; ;
Issue Date:
Research Org.:
Board of Trustees of Michigan State University, East Lansing, MI (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1250460
Patent Number(s):
9,328,335
Application Number:
13/519,660
Assignee:
Board of Trustees of Michigan State University (East Lansing, MI)
DOE Contract Number:  
FC02-07ER64494
Resource Type:
Patent
Resource Relation:
Patent File Date: 2010 Dec 29
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Durrett, Timothy, Ohlrogge, John, and Pollard, Michael. Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush). United States: N. p., 2016. Web.
Durrett, Timothy, Ohlrogge, John, & Pollard, Michael. Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush). United States.
Durrett, Timothy, Ohlrogge, John, and Pollard, Michael. Tue . "Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush)". United States. https://www.osti.gov/servlets/purl/1250460.
@article{osti_1250460,
title = {Method to produce acetyldiacylglycerols (ac-TAGs) by expression of an acetyltransferase gene isolated from Euonymus alatus (burning bush)},
author = {Durrett, Timothy and Ohlrogge, John and Pollard, Michael},
abstractNote = {The present invention relates to novel diacylglycerol acyltransferase genes and proteins, and methods of their use. In particular, the invention describes genes encoding proteins having diacylglycerol acetyltransferase activity, specifically for transferring an acetyl group to a diacylglycerol substrate to form acetyl-Triacylglycerols (ac-TAGS), for example, a 3-acetyl-1,2-diacyl-sn-glycerol. The present invention encompasses both native and recombinant wild-type forms of the transferase, as well as mutants and variant forms. The present invention also relates to methods of using novel diacylglycerol acyltransferase genes and proteins, including their expression in transgenic organisms at commercially viable levels, for increasing production of 3-acetyl-1,2-diacyl-sn-glycerols in plant oils and altering the composition of oils produced by microorganisms, such as yeast, by increasing ac-TAG production. Additionally, oils produced by methods of the present inventions comprising genes and proteins are contemplated for use as biodiesel fuel, in polymer production and as naturally produced food oils with reduced calories.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2016},
month = {5}
}

Patent:

Save / Share:

Works referenced in this record:

Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase: Plant cDNAs encoding diacylglycerol acyltransferase
journal, January 2000


Automated protein function prediction--the genomic challenge
journal, May 2006

  • Friedberg, I.
  • Briefings in Bioinformatics, Vol. 7, Issue 3, p. 225-242
  • DOI: 10.1093/bib/bbl004

Functional Analysis of Conserved Histidines in ADP-Glucose Pyrophosphorylase from Escherichia coli
journal, March 1998

  • Hill, Margaret A.; Preiss, Jack
  • Biochemical and Biophysical Research Communications, Vol. 244, Issue 2, p. 573-577
  • DOI: 10.1006/bbrc.1998.8301

Protein tolerance to random amino acid change
journal, June 2004

  • Guo, H. H.; Choe, J.; Loeb, L. A.
  • Proceedings of the National Academy of Sciences, Vol. 101, Issue 25, p. 9205-9210
  • DOI: 10.1073/pnas.0403255101

Rapid cDNA sequencing (expressed sequence tags) from a directionally cloned human infant brain cDNA library
journal, August 1993

  • Adams, Mark D.; Soares, M. Bento; Kerlavage, Anthony R.
  • Nature Genetics, Vol. 4, Issue 4
  • DOI: 10.1038/ng0893-373

Efficient functioning of plant promoters and poly(A) sites in Xenopus oocytes
journal, January 1989

  • Ballas, Nurit; Broido, Shimshon; Soreq, Hermona
  • Nucleic Acids Research, Vol. 17, Issue 19
  • DOI: 10.1093/nar/17.19.7891

An integrated overview of seed development in Arabidopsis thaliana ecotype WS
journal, February 2002

  • Baud, Sébastien; Boutin, Jean-Pierre; Miquel, Martine
  • Plant Physiology and Biochemistry, Vol. 40, Issue 2, p. 151-160
  • DOI: 10.1016/S0981-9428(01)01350-X

Identification of differentially expressed mRNA species by an improved disply technique (DDRT-PCR)
journal, January 1993

  • Bauer, David; Muüller, Heiko; Reich, Jens
  • Nucleic Acids Research, Vol. 21, Issue 18
  • DOI: 10.1093/nar/21.18.4272

An analysis of differential display shows a strong bias towards high copy number mRNAs
journal, January 1995

  • Bertioli, David J.; Schlichter, Ursula H. A.; Adams, Michael J.
  • Nucleic Acids Research, Vol. 23, Issue 21
  • DOI: 10.1093/nar/23.21.4520

A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation
journal, July 1983

  • Bevan, Michael W.; Flavell, Richard B.; Chilton, Mary-Dell
  • Nature, Vol. 304, Issue 5922
  • DOI: 10.1038/304184a0

A Rapid Method of Total Lipid Extraction and Purification
journal, August 1959

  • Bligh, E. G.; Dyer, W. J.
  • Canadian Journal of Biochemistry and Physiology, Vol. 37, Issue 8
  • DOI: 10.1139/o59-099

Hygromycin B phosphotransferase as a selectable marker for DNA transfer experiments with higher eucaryotic cells.
journal, December 1984

  • Blochlinger, K.; Diggelmann, H.
  • Molecular and Cellular Biology, Vol. 4, Issue 12
  • DOI: 10.1128/MCB.4.12.2929

Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil
journal, October 2008


Introns increase gene expression in cultured maize cells.
journal, December 1987

  • Callis, J.; Fromm, M.; Walbot, V.
  • Genes & Development, Vol. 1, Issue 10, p. 1183-1200
  • DOI: 10.1101/gad.1.10.1183

Transgenic sorghum plants via microprojectile bombardment
journal, December 1993

  • Casas, A. M.; Kononowicz, A. K.; Zehr, U. B.
  • Proceedings of the National Academy of Sciences, Vol. 90, Issue 23
  • DOI: 10.1073/pnas.90.23.11212

New RNA Polymerase from Escherichia coli infected with Bacteriophage T7
journal, October 1970

  • Chamberlin, Michael; McGrath, Janet; Waskell, Lucy
  • Nature, Vol. 228, Issue 5268, p. 227-231
  • DOI: 10.1038/228227a0

Biosynthesis of Phytosterol Esters: Identification of a Sterol O -Acyltransferase in Arabidopsis
journal, September 2007

  • Chen, Qilin; Steinhauer, Lee; Hammerlindl, Joe
  • Plant Physiology, Vol. 145, Issue 3
  • DOI: 10.1104/pp.107.106278

Stable Transformation of Soybean Callus by DNA-Coated Gold Particles
journal, July 1988

  • Christou, Paul; McCabe, Dennis E.; Swain, William F.
  • Plant Physiology, Vol. 87, Issue 3
  • DOI: 10.1104/pp.87.3.671

Integration of foreign DNA following microinjection of tobacco mesophyll protoplasts
journal, February 1986

  • Crossway, Anne; Oakes, Janette V.; Irvine, Jonathan M.
  • MGG Molecular & General Genetics, Vol. 202, Issue 2
  • DOI: 10.1007/BF00331634

Phospholipid:diacylglycerol acyltransferase: An enzyme that catalyzes the acyl-CoA-independent formation of triacylglycerol in yeast and plants
journal, May 2000

  • Dahlqvist, A.; Stahl, U.; Lenman, M.
  • Proceedings of the National Academy of Sciences, Vol. 97, Issue 12, p. 6487-6492
  • DOI: 10.1073/pnas.120067297

Genetically Engineered Fertile Indica-Rice Recovered from Protoplasts
journal, August 1990

  • Datta, Swapan K.; Peterhans, Alex; Datta, Karabi
  • Nature Biotechnology, Vol. 8, Issue 8
  • DOI: 10.1038/nbt0890-736

Production of high levels of 8:0 and 10:0 fatty acids in transgenic canola by overexpression of Ch FatB2, a thioesterase cDNA from Cuphea hookeriana
journal, February 1996


Two Novel Thioesterases Are Key Determinants of the Bimodal Distribution of Acyl Chain Length of Cuphea palustris Seed Oil
journal, January 1996


A distinct DGAT with sn-3 acetyltransferase activity that synthesizes unusual, reduced-viscosity oils in Euonymus and transgenic seeds
journal, May 2010

  • Durrett, T. P.; McClosky, D. D.; Tumaney, A. W.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 20, p. 9464-9469
  • DOI: 10.1073/pnas.1001707107

Plant triacylglycerols as feedstocks for the production of biofuels
journal, May 2008


Development and potential of genetically engineered oilseeds
journal, December 2005

  • Dyer, John M.; Mullen, Robert T.
  • Seed Science Research, Vol. 15, Issue 4
  • DOI: 10.1079/SSR2005216

Liposome-mediated delivery of tobacco mosaic virus RNA into tobacco protoplasts: A sensitive assay for monitoring liposome-protoplast interactions
journal, March 1982

  • Fraley, R. T.; Dellaporta, S. L.; Papahadjopoulos, D.
  • Proceedings of the National Academy of Sciences, Vol. 79, Issue 6
  • DOI: 10.1073/pnas.79.6.1859

Expression of genes transferred into monocot and dicot plant cells by electroporation.
journal, September 1985

  • Fromm, M.; Taylor, L. P.; Walbot, V.
  • Proceedings of the National Academy of Sciences, Vol. 82, Issue 17, p. 5824-5828
  • DOI: 10.1073/pnas.82.17.5824

Inheritance and Expression of Chimeric Genes in the Progeny of Transgenic Maize Plants
journal, September 1990

  • Fromm, Michael E.; Morrish, Fionnuala; Armstrong, Charles
  • Nature Biotechnology, Vol. 8, Issue 9
  • DOI: 10.1038/nbt0990-833

Isolation of a ubiquitin-ribosomal protein gene (ubi3) from potato and expression of its promoter in transgenic plants
journal, January 1994

  • Garbarino, Joan E.; Belknap, William R.
  • Plant Molecular Biology, Vol. 24, Issue 1
  • DOI: 10.1007/BF00040579

Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants.
journal, July 1990


Effect of deletions in the cauliflower mosaic virus polyadenylation sequence on the choice of the polyadenylation sites in tobacco protoplasts
journal, April 1991

  • Guerineau, Fran�ois; Brooks, Louise; Mullineaux, Philip
  • MGG Molecular & General Genetics, Vol. 226-226, Issue 1-2
  • DOI: 10.1007/BF00273597

Amplified Restriction Fragment Length Polymorphism-Based mRNA Fingerprinting Using a Single Restriction Enzyme That Recognizes a 4-bp Sequence
journal, May 1997

  • Habu, Yoshiki; Fukada-Tanaka, Sachiko; Hisatomi, Yasuyo
  • Biochemical and Biophysical Research Communications, Vol. 234, Issue 2
  • DOI: 10.1006/bbrc.1997.6666

A Polyethylene Glycol-Mediated Protoplast Transformation System for Production of Fertile Transgenic Rice Plants
journal, July 1990

  • Hayashimoto, Akio; Li, Zhijian; Murai, Norimoto
  • Plant Physiology, Vol. 93, Issue 3
  • DOI: 10.1104/pp.93.3.857

Cloning and characterization of a cDNA encoding diacylglycerol acyltransferase from castor bean
journal, April 2004


Regulation of diacylglycerol acyltransferase in developing seeds of castor
journal, September 2004


Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins
journal, March 1984

  • Hedrick, Stephen M.; Nielsen, Ellen A.; Kavaler, Joshua
  • Nature, Vol. 308, Issue 5955
  • DOI: 10.1038/308153a0

Production of Transgenic Soybean Plants Using Agrobacterium-Mediated DNA Transfer
journal, August 1988

  • Hinchee, Maud A. W.; Connor-Ward, Dannette V.; Newell, Christine A.
  • Nature Biotechnology, Vol. 6, Issue 8
  • DOI: 10.1038/nbt0888-915

Cloning of a cDNA encoding diacylglycerol acyltransferase from Arabidopsis thaliana and its functional expression
journal, June 1999


Diacylglycerol acyltransferase in maturing safflower seeds: its influences on the fatty acid composition of triacylglycerol and on the rate of triacylglycerol synthesis
journal, January 1988

  • Ichihara, Ken'ichi; Takahashi, Toshiaki; Fujii, Shoji
  • Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, Vol. 958, Issue 1, p. 125-129
  • DOI: 10.1016/0005-2760(88)90253-6

High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens
journal, June 1996

  • Ishida, Yuji; Saito, Hideaki; Ohta, Shozo
  • Nature Biotechnology, Vol. 14, Issue 6, p. 745-750
  • DOI: 10.1038/nbt0696-745

Fluorescent differential display: Arbitrarily primed RT-PCR fingerprinting on an automated DNA sequencer
journal, September 1994


Regeneration of transgenic, microspore-derived, fertile barley
journal, October 1994

  • Jähne, A.; Becker, D.; Brettschneider, R.
  • Theoretical and Applied Genetics, Vol. 89, Issue 4
  • DOI: 10.1007/BF00225390

Seed-Specific Over-Expression of an Arabidopsis cDNA Encoding a Diacylglycerol Acyltransferase Enhances Seed Oil Content and Seed Weight
journal, June 2001

  • Jako, Colette; Kumar, Arvind; Wei, Yangdou
  • Plant Physiology, Vol. 126, Issue 2
  • DOI: 10.1104/pp.126.2.861

Industrial oils from transgenic plants
journal, April 2003


Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis
journal, January 1987


A Replicating RNA Molecule Suitable for a Detailed Analysis of Extracellular Evolution and Replication
journal, October 1972

  • Kacian, D. L.; Mills, D. R.; Kramer, F. R.
  • Proceedings of the National Academy of Sciences, Vol. 69, Issue 10
  • DOI: 10.1073/pnas.69.10.3038

A Novel Bifunctional Wax Ester Synthase/Acyl-CoA:Diacylglycerol Acyltransferase Mediates Wax Ester and Triacylglycerol Biosynthesis in Acinetobacter calcoaceticus ADP1
journal, December 2002

  • Kalscheuer, Rainer; Steinbüchel, Alexander
  • Journal of Biological Chemistry, Vol. 278, Issue 10, p. 8075-8082
  • DOI: 10.1074/jbc.M210533200

Increased fatty acid production in potato by engineering of acetyl-CoA carboxylase
journal, March 2004


Factors Influencing Gene Delivery into Zea Mays Cells by High–Velocity Microprojectiles
journal, May 1988

  • Klein, T. M.; Gradziel, T.; Fromm, M. E.
  • Nature Biotechnology, Vol. 6, Issue 5
  • DOI: 10.1038/nbt0588-559

Genetic Transformation of Maize Cells by Particle Bombardment
journal, September 1989

  • Klein, Theodore M.; Kornstein, Laura; Sanford, John C.
  • Plant Physiology, Vol. 91, Issue 1
  • DOI: 10.1104/pp.91.1.440

Transfer of foreign genes into intact maize cells with high-velocity microprojectiles
journal, June 1988

  • Klein, T. M.; Fromm, M.; Weissinger, A.
  • Proceedings of the National Academy of Sciences, Vol. 85, Issue 12
  • DOI: 10.1073/pnas.85.12.4305

Transformation of the developing barley endosperm by particle bombardment
journal, October 1991


Field Performance of Elite Transgenic Maize Plants Expressing an Insecticidal Protein Derived from Bacillus thuringiensis
journal, February 1993

  • Koziel, Michael G.; Beland, Gary L.; Bowman, Cindy
  • Nature Biotechnology, Vol. 11, Issue 2
  • DOI: 10.1038/nbt0293-194

Transgenic Maize for the Control of European Corn Borer and Other Maize Insect Pests
journal, May 1996

  • Koziel, Michael G.; Carozzi, Nadine B.; Desai, Nalini
  • Annals of the New York Academy of Sciences, Vol. 792, Issue 1 Engineering P
  • DOI: 10.1111/j.1749-6632.1996.tb32502.x

In vitro transformation of plant protoplasts with Ti-plasmid DNA
journal, March 1982

  • Krens, F. A.; Molendijk, L.; Wullems, G. J.
  • Nature, Vol. 296, Issue 5852
  • DOI: 10.1038/296072a0

DGAT2 Is a New Diacylglycerol Acyltransferase Gene Family: PURIFICATION, CLONING, AND EXPRESSION IN INSECT CELLS OF TWO POLYPEPTIDES FROM
journal, July 2001

  • Lardizabal, Kathryn D.; Mai, Jennifer T.; Wagner, Nicholas W.
  • Journal of Biological Chemistry, Vol. 276, Issue 42
  • DOI: 10.1074/jbc.M106168200

Purification of a Jojoba Embryo Wax Synthase, Cloning of its cDNA, and Production of High Levels of Wax in Seeds of Transgenic Arabidopsis
journal, March 2000

  • Lardizabal, Kathryn D.; Metz, James G.; Sakamoto, Tetsuo
  • Plant Physiology, Vol. 122, Issue 3
  • DOI: 10.1104/pp.122.3.645

Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction
journal, August 1992


Distribution and cloning of eukaryotic mRNAs by means of differential display: refinements and optimization
journal, January 1993


Regulation of inducible and tissue-specific gene expression
journal, June 1987

  • Maniatis, T.; Goodbourn, S.; Fischer, J. A.
  • Science, Vol. 236, Issue 4806, p. 1237-1245
  • DOI: 10.1126/science.3296191

Stable Transformation of Soybean (Glycine Max) by Particle Acceleration
journal, August 1988

  • McCabe, Dennis E.; Swain, William F.; Martinell, Brian J.
  • Nature Biotechnology, Vol. 6, Issue 8
  • DOI: 10.1038/nbt0888-923

Seed-Specific Heterologous Expression of a Nasturtium FAE Gene in Arabidopsis Results in a Dramatic Increase in the Proportion of Erucic Acid
journal, August 2004

  • Mietkiewska, Elzbieta; Giblin, E. Michael; Wang, Song
  • Plant Physiology, Vol. 136, Issue 1
  • DOI: 10.1104/pp.104.046839

Isolation of a Gene Encoding a 1,2-Diacylglycerol- sn -acetyl-CoA Acetyltransferase from Developing Seeds of Euonymus alatus
journal, December 2004

  • Milcamps, Anne; Tumaney, Ajay W.; Paddock, Troy
  • Journal of Biological Chemistry, Vol. 280, Issue 7
  • DOI: 10.1074/jbc.M410276200

Upstream sequences other than AAUAAA are required for efficient messenger RNA 3'-end formation in plants.
journal, December 1990

  • Mogen, B. D.; MacDonald, M. H.; Graybosch, R.
  • The Plant Cell, Vol. 2, Issue 12
  • DOI: 10.1105/tpc.2.12.1261

Tales of poly(A): a review
journal, July 1990


Production of novel oils in plants
journal, April 1999


Characterization of cDNAs encoding diacylglycerol acyltransferase from cultures of Brassica napus and sucrose-mediated induction of enzyme biosynthesis
journal, February 2002

  • Nykiforuk, C.
  • Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, Vol. 1580, Issue 2-3
  • DOI: 10.1016/S1388-1981(01)00200-1

Identification of DNA sequences required for activity of the cauliflower mosaic virus 35S promoter
journal, February 1985

  • Odell, Joan T.; Nagy, Ferenc; Chua, Nam-Hai
  • Nature, Vol. 313, Issue 6005, p. 810-812
  • DOI: 10.1038/313810a0

Lipid Biosynthesis
journal, July 1995

  • Ohlrogge, J.; Browse, J.
  • THE PLANT CELL ONLINE, Vol. 7, Issue 7, p. 957-970
  • DOI: 10.1105/tpc.7.7.957

Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression
journal, November 1992

  • Okubo, Kousaku; Hori, Naohiro; Matoba, Ryo
  • Nature Genetics, Vol. 2, Issue 3
  • DOI: 10.1038/ng1192-173

Poly(A) signals
journal, February 1991


Stable transformation of tobacco by electroporation: evidence for plasmid concatenation.
journal, August 1986

  • Riggs, C. D.; Bates, G. W.
  • Proceedings of the National Academy of Sciences, Vol. 83, Issue 15
  • DOI: 10.1073/pnas.83.15.5602

Vectors for selective expression of cloned DNAs by T7 RNA polymerase
journal, January 1987


The TAG1 locus of Arabidopsis encodes for a diacylglycerol acyltransferase
journal, November 1999

  • Routaboul, Jean-Marc; Benning, Christoph; Bechtold, Nicole
  • Plant Physiology and Biochemistry, Vol. 37, Issue 11, p. 831-840
  • DOI: 10.1016/S0981-9428(99)00115-1

Cytosolic Triacylglycerol Biosynthetic Pathway in Oilseeds. Molecular Cloning and Expression of Peanut Cytosolic Diacylglycerol Acyltransferase
journal, June 2006

  • Saha, Saikat; Enugutti, Balaji; Rajakumari, Sona
  • Plant Physiology, Vol. 141, Issue 4
  • DOI: 10.1104/pp.106.082198

Storage Lipid Synthesis Is Non-essential in Yeast
journal, December 2001

  • Sandager, Line; Gustavsson, Maria H.; Ståhl, Ulf
  • Journal of Biological Chemistry, Vol. 277, Issue 8, p. 6478-6482
  • DOI: 10.1074/jbc.M109109200

A dissection of the cauliflower mosaic virus polyadenylation signal.
journal, January 1991

  • Sanfacon, H.; Brodmann, P.; Hohn, T.
  • Genes & Development, Vol. 5, Issue 1
  • DOI: 10.1101/gad.5.1.141

Delivery of Substances into Cells and Tissues Using a Particle Bombardment Process
journal, January 1987

  • Sanford, John C.; Klein, Theodore M.; Wolf, Edward D.
  • Particulate Science and Technology, Vol. 5, Issue 1
  • DOI: 10.1080/02726358708904533

Transgenic Plants as Tools to Study the Molecular Organization of Plant Genes
journal, September 1987


Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray
journal, October 1995


Desaturation and Related Modifications of Fatty Acids
journal, June 1998


Fertile transgenic rice plants regenerated from transformed protoplasts
journal, March 1989

  • Shimamoto, Ko; Terada, Rie; Izawa, Takeshi
  • Nature, Vol. 338, Issue 6212, p. 274-276
  • DOI: 10.1038/338274a0

Gene expression analysis by transcript profiling coupled to a gene database query
journal, August 1999

  • Shimkets, Richard A.; Lowe, David G.; Tai, Julie Tsu-Ning
  • Nature Biotechnology, Vol. 17, Issue 8
  • DOI: 10.1038/11743

Tung Tree DGAT1 and DGAT2 Have Nonredundant Functions in Triacylglycerol Biosynthesis and Are Localized to Different Subdomains of the Endoplasmic Reticulum
journal, August 2006

  • Shockey, Jay M.; Gidda, Satinder K.; Chapital, Dorselyn C.
  • The Plant Cell, Vol. 18, Issue 9
  • DOI: 10.1105/tpc.106.043695

Metabolic engineering of new fatty acids in plants
journal, April 2005

  • Singh, Surinder P.; Zhou, Xue-Rong; Liu, Qing
  • Current Opinion in Plant Biology, Vol. 8, Issue 2, p. 197-203
  • DOI: 10.1016/j.pbi.2005.01.012

Obesity resistance and multiple mechanisms of triglyceride synthesis in mice lacking Dgat
journal, May 2000

  • Smith, Steven J.; Cases, Sylvaine; Jensen, Dalan R.
  • Nature Genetics, Vol. 25, Issue 1
  • DOI: 10.1038/75651

Fertile, Transgenic Oat Plants
journal, December 1992

  • Somers, David A.; Rines, Howard W.; Gu, Weining
  • Nature Biotechnology, Vol. 10, Issue 12
  • DOI: 10.1038/nbt1292-1589

A method for examining expression of homologous genes in plant polyploids
journal, November 1994

  • Song, Keming; Osborn, Thomas C.
  • Plant Molecular Biology, Vol. 26, Issue 4
  • DOI: 10.1007/BF00040689

Bialaphos selection of stable transformants from maize cell culture
journal, May 1990

  • Spencer, T. M.; Gordon-Kamm, W. J.; Daines, R. J.
  • Theoretical and Applied Genetics, Vol. 79, Issue 5
  • DOI: 10.1007/BF00226875

Cloning and Functional Characterization of a Phospholipid:Diacylglycerol Acyltransferase from Arabidopsis
journal, July 2004

  • Ståhl, Ulf; Carlsson, Anders S.; Lenman, Marit
  • Plant Physiology, Vol. 135, Issue 3
  • DOI: 10.1104/pp.104.044354

Long regions of homologous DNA are incorporated into the tobacco plastid genome by transformation.
journal, January 1992


Lipopenia and Skin Barrier Abnormalities in DGAT2-deficient Mice
journal, December 2003

  • Stone, Scot J.; Myers, Heather M.; Watkins, Steven M.
  • Journal of Biological Chemistry, Vol. 279, Issue 12
  • DOI: 10.1074/jbc.M311000200

High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene.
journal, February 1993

  • Svab, Z.; Maliga, P.
  • Proceedings of the National Academy of Sciences, Vol. 90, Issue 3
  • DOI: 10.1073/pnas.90.3.913

Stable transformation of plastids in higher plants.
journal, November 1990

  • Svab, Z.; Hajdukiewicz, P.; Maliga, P.
  • Proceedings of the National Academy of Sciences, Vol. 87, Issue 21
  • DOI: 10.1073/pnas.87.21.8526

Metabolic Engineering of Fatty Acid Biosynthesis in Plants
journal, January 2002


Use of paromomycin as a selective agent for oat transformation
journal, July 1995

  • Torbert, Kimberly A.; Rines, Howard W.; Somers, David A.
  • Plant Cell Reports, Vol. 14, Issue 10
  • DOI: 10.1007/BF00232728

Genetically Transformed Cotton (Gossypium Hirsutum L.) Plants
journal, March 1987

  • Umbeck, Paul; Johnson, Gail; Barton, Ken
  • Bio/Technology, Vol. 5, Issue 3
  • DOI: 10.1038/nbt0387-263

Rapid Production of Transgenic Wheat Plants by Direct Bombardment of Cultured Immature Embryos
journal, December 1993

  • Vasil, Vimla; Srivastava, Vibha; Castillo, Ana M.
  • Nature Biotechnology, Vol. 11, Issue 12
  • DOI: 10.1038/nbt1293-1553

Serial Analysis of Gene Expression
journal, October 1995


The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers
journal, October 1982


The role of enhancers in the regulation of cell-type-specific transcriptional control
journal, July 1986

  • Voss, Stephan D.; Schlokat, Uwe; Gruss, Peter
  • Trends in Biochemical Sciences, Vol. 11, Issue 7, p. 287-289
  • DOI: 10.1016/0968-0004(86)90031-9

A cassette containing the bar gene of Streptomyces hygroscopicus : a selectable marker for plant transformation
journal, January 1990

  • White, Janet; Chang, Sheng-Yung P.; Bibb, Maureen J.
  • Nucleic Acids Research, Vol. 18, Issue 4
  • DOI: 10.1093/nar/18.4.1062

Thematic Review Series: Glycerolipids. DGAT enzymes and triacylglycerol biosynthesis
journal, August 2008

  • Yen, Chi-Liang Eric; Stone, Scot J.; Koliwad, Suneil
  • Journal of Lipid Research, Vol. 49, Issue 11
  • DOI: 10.1194/jlr.R800018-JLR200

Gene Expression Profiles in Normal and Cancer Cells
journal, May 1997


Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene.
journal, June 1997


The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene
journal, September 1999


The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene
journal, September 1999