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Title: Modified cellulose synthase gene from Arabidopsis thaliana confers herbicide resistance to plants

Abstract

Cellulose synthase ("CS"), a key enzyme in the biosynthesis of cellulose in plants is inhibited by herbicides comprising thiazolidinones such as 5-tert-butyl-carbamoyloxy-3-(3-trifluromethyl)phenyl-4-thiazolidinone (TZ), isoxaben and 2,6-dichlorobenzonitrile (DCB). Two mutant genes encoding isoxaben and TZ-resistant cellulose synthase have been isolated from isoxaben and TZ-resistant Arabidopsis thaliana mutants. When compared with the gene coding for isoxaben or TZ-sensitive cellulose synthase, one of the resistant CS genes contains a point mutation, wherein glycine residue 998 is replaced by an aspartic acid. The other resistant mutation is due to a threonine to isoleucine change at amino acid residue 942. The mutant CS gene can be used to impart herbicide resistance to a plant; thereby permitting the utilization of the herbicide as a single application at a concentration which ensures the complete or substantially complete killing of weeds, while leaving the transgenic crop plant essentially undamaged.

Inventors:
 [1];  [2]
  1. Portola Valley, CA
  2. Golm, DE
Issue Date:
Research Org.:
Carnegie Institute of Washington
Sponsoring Org.:
USDOE
OSTI Identifier:
912868
Patent Number(s):
7241878
Application Number:
10/721,996
Assignee:
U.S. Department of Energy (Washington, DC)
Patent Classifications (CPCs):
C - CHEMISTRY C12 - BIOCHEMISTRY C12N - MICROORGANISMS OR ENZYMES
DOE Contract Number:  
FG02-94ER20133
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Somerville, Chris R, and Scheible, Wolf. Modified cellulose synthase gene from Arabidopsis thaliana confers herbicide resistance to plants. United States: N. p., 2007. Web.
Somerville, Chris R, & Scheible, Wolf. Modified cellulose synthase gene from Arabidopsis thaliana confers herbicide resistance to plants. United States.
Somerville, Chris R, and Scheible, Wolf. Tue . "Modified cellulose synthase gene from Arabidopsis thaliana confers herbicide resistance to plants". United States. https://www.osti.gov/servlets/purl/912868.
@article{osti_912868,
title = {Modified cellulose synthase gene from Arabidopsis thaliana confers herbicide resistance to plants},
author = {Somerville, Chris R and Scheible, Wolf},
abstractNote = {Cellulose synthase ("CS"), a key enzyme in the biosynthesis of cellulose in plants is inhibited by herbicides comprising thiazolidinones such as 5-tert-butyl-carbamoyloxy-3-(3-trifluromethyl)phenyl-4-thiazolidinone (TZ), isoxaben and 2,6-dichlorobenzonitrile (DCB). Two mutant genes encoding isoxaben and TZ-resistant cellulose synthase have been isolated from isoxaben and TZ-resistant Arabidopsis thaliana mutants. When compared with the gene coding for isoxaben or TZ-sensitive cellulose synthase, one of the resistant CS genes contains a point mutation, wherein glycine residue 998 is replaced by an aspartic acid. The other resistant mutation is due to a threonine to isoleucine change at amino acid residue 942. The mutant CS gene can be used to impart herbicide resistance to a plant; thereby permitting the utilization of the herbicide as a single application at a concentration which ensures the complete or substantially complete killing of weeds, while leaving the transgenic crop plant essentially undamaged.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2007},
month = {7}
}

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Works referenced in this record:

A Second Locus, Ixr B1 in Arabidopsis thaliana, that Confers Resistance to the Herbicide Isoxaben
journal, March 1990


Modifications of cellulose synthase confer resistance to isoxaben and thiazolidinone herbicides in Arabidopsis Ixr1 mutants
journal, August 2001