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Title: Sampling probe for microarray read out using electrospray mass spectrometry

Abstract

An automated electrospray based sampling system and method for analysis obtains samples from surface array spots having analytes. The system includes at least one probe, the probe including an inlet for flowing at least one eluting solvent to respective ones of a plurality of spots and an outlet for directing the analyte away from the spots. An automatic positioning system is provided for translating the probe relative to the spots to permit sampling of any spot. An electrospray ion source having an input fluidicly connected to the probe receives the analyte and generates ions from the analyte. The ion source provides the generated ions to a structure for analysis to identify the analyte, preferably being a mass spectrometer. The probe can be a surface contact probe, where the probe forms an enclosing seal along the periphery of the array spot surface.

Inventors:
Issue Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1175073
Patent Number(s):
6803566
Application Number:
10/123,629
Assignee:
UT-Battelle, LLC (Oak Ridge, TN)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
H - ELECTRICITY H01 - BASIC ELECTRIC ELEMENTS H01J - ELECTRIC DISCHARGE TUBES OR DISCHARGE LAMPS
DOE Contract Number:  
AC05-00OR22725
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Van Berkel, Gary J. Sampling probe for microarray read out using electrospray mass spectrometry. United States: N. p., 2004. Web.
Van Berkel, Gary J. Sampling probe for microarray read out using electrospray mass spectrometry. United States.
Van Berkel, Gary J. Tue . "Sampling probe for microarray read out using electrospray mass spectrometry". United States. https://www.osti.gov/servlets/purl/1175073.
@article{osti_1175073,
title = {Sampling probe for microarray read out using electrospray mass spectrometry},
author = {Van Berkel, Gary J.},
abstractNote = {An automated electrospray based sampling system and method for analysis obtains samples from surface array spots having analytes. The system includes at least one probe, the probe including an inlet for flowing at least one eluting solvent to respective ones of a plurality of spots and an outlet for directing the analyte away from the spots. An automatic positioning system is provided for translating the probe relative to the spots to permit sampling of any spot. An electrospray ion source having an input fluidicly connected to the probe receives the analyte and generates ions from the analyte. The ion source provides the generated ions to a structure for analysis to identify the analyte, preferably being a mass spectrometer. The probe can be a surface contact probe, where the probe forms an enclosing seal along the periphery of the array spot surface.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {2004},
month = {10}
}

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Works referenced in this record:

A Fully Integrated Monolithic Microchip Electrospray Device for Mass Spectrometry
journal, September 2000


High-Throughput Microfabricated CE/ESI-MS:  Automated Sampling from a Microwell Plate
journal, June 2001


Error-Tolerant Identification of Peptides in Sequence Databases by Peptide Sequence Tags
journal, December 1994


Automated High-Throughput Infusion ESI-MS with Direct Coupling to a Microtiter Plate
journal, April 2001


Identification of Bacteriophage MS2 Coat Protein from E. coli Lysates via Ion Trap Collisional Activation of Intact Protein Ions
journal, March 2001