Title: Study of polymer molecules and conformations with a nanopore

Abstract

The invention features methods for evaluating the conformation of a polymer, for example, for determining the conformational distribution of a plurality of polymers and to detect binding or denaturation events. The methods employ a nanopore which the polymer, e.g., a nucleic acid, traverses. As the polymer traverses the nanopore, measurements of transport properties of the nanopore yield data on the conformation of the polymer.

Inventors:

Golovchenko, Jene A ^[1]; Li, Jiali ^[2]; Stein, Derek ^[3]; Gershow, Marc H ^[4]

---

+ Show Inventor Affiliations

1. Lexington, MA
2. Fayetteville, AR
3. Delft, NL
4. Cambridge, MA

Issue Date:

Tue Dec 07 00:00:00 EST 2010

Research Org.:

Harvard College

Sponsoring Org.:

USDOE

OSTI Identifier:

1011416

Patent Number(s):

7846738

Application Number:

10/918,959

Assignee:

CHO

Patent Classifications (CPCs):

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

Show more

C - CHEMISTRY C12 - BIOCHEMISTRY C12Q - MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS

C12Q1/6811 - Selection methods for production or design of target specific oligonucleotides or binding molecules
C12Q2563/143 - Magnetism, e.g. magnetic label
C12Q2565/133 - conformational analysis
C12Q2565/629 - being a microfluidic device
C12Q2565/631 - being a biochannel or pore

G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES

G01N33/48721 - {Investigating individual macromolecules, e.g. by translocation through nanopores
G01N33/50 - Chemical analysis of biological material, e.g. blood, urine

Y - NEW / CROSS SECTIONAL TECHNOLOGIES Y10 - TECHNICAL SUBJECTS COVERED BY FORMER USPC Y10T - TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION

Y10T436/14 - Heterocyclic carbon compound [i.e. , O, S, N, Se, Te, as only ring hetero atom]
Y10T436/142222 - Hetero-O [e.g., ascorbic acid, etc.]
Y10T436/143333 - Saccharide [e.g., DNA, etc.]

Show less

DOE Contract Number:  

US patent application 10/918,959

Resource Type:

Patent

Country of Publication:

United States

Language:

English