Multiple capillary biochemical analyzer
Abstract
A multiple capillary analyzer allows detection of light from multiple capillaries with a reduced number of interfaces through which light must pass in detecting light emitted from a sample being analyzed, using a modified sheath flow cuvette. A linear or rectangular array of capillaries is introduced into a rectangular flow chamber. Sheath fluid draws individual sample streams through the cuvette. The capillaries are closely and evenly spaced and held by a transparent retainer in a fixed position in relation to an optical detection system. Collimated sample excitation radiation is applied simultaneously across the ends of the capillaries in the retainer. Light emitted from the excited sample is detected by the optical detection system. The retainer is provided by a transparent chamber having inward slanting end walls. The capillaries are wedged into the chamber. One sideways dimension of the chamber is equal to the diameter of the capillaries and one end to end dimension varies from, at the top of the chamber, slightly greater than the sum of the diameters of the capillaries to, at the bottom of the chamber, slightly smaller than the sum of the diameters of the capillaries. The optical system utilizes optic fibers to deliver light tomore »
- Inventors:
- Issue Date:
- Research Org.:
- University of Alberta
- OSTI Identifier:
- 100979
- Patent Number(s):
- 5439578
- Application Number:
- PAN: 8-072,096
- Assignee:
- Univ. of Alberta, Edmonton, AB (Canada)
- DOE Contract Number:
- FG02-91ER61123
- Resource Type:
- Patent
- Resource Relation:
- Other Information: PBD: 8 Aug 1995
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 55 BIOLOGY AND MEDICINE, BASIC STUDIES; CAPILLARIES; BIOCHEMISTRY; OPTICAL EQUIPMENT; DESIGN; MEDICAL SUPPLIES; CHEMICAL ANALYSIS; FLUID FLOW; FIBER OPTICS
Citation Formats
Dovichi, N J, and Zhang, J Z. Multiple capillary biochemical analyzer. United States: N. p., 1995.
Web.
Dovichi, N J, & Zhang, J Z. Multiple capillary biochemical analyzer. United States.
Dovichi, N J, and Zhang, J Z. Tue .
"Multiple capillary biochemical analyzer". United States.
@article{osti_100979,
title = {Multiple capillary biochemical analyzer},
author = {Dovichi, N J and Zhang, J Z},
abstractNote = {A multiple capillary analyzer allows detection of light from multiple capillaries with a reduced number of interfaces through which light must pass in detecting light emitted from a sample being analyzed, using a modified sheath flow cuvette. A linear or rectangular array of capillaries is introduced into a rectangular flow chamber. Sheath fluid draws individual sample streams through the cuvette. The capillaries are closely and evenly spaced and held by a transparent retainer in a fixed position in relation to an optical detection system. Collimated sample excitation radiation is applied simultaneously across the ends of the capillaries in the retainer. Light emitted from the excited sample is detected by the optical detection system. The retainer is provided by a transparent chamber having inward slanting end walls. The capillaries are wedged into the chamber. One sideways dimension of the chamber is equal to the diameter of the capillaries and one end to end dimension varies from, at the top of the chamber, slightly greater than the sum of the diameters of the capillaries to, at the bottom of the chamber, slightly smaller than the sum of the diameters of the capillaries. The optical system utilizes optic fibers to deliver light to individual photodetectors, one for each capillary tube. A filter or wavelength division demultiplexer may be used for isolating fluorescence at particular bands. 21 figs.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Aug 08 00:00:00 EDT 1995},
month = {Tue Aug 08 00:00:00 EDT 1995}
}