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Title: Multiple capillary biochemical analyzer

A multiple capillary analyzer allows detection of light from multiple capillaries with a reduced number of interfaces through which light must pass in detecting light emitted from a sample being analyzed, using a modified sheath flow cuvette. A linear or rectangular array of capillaries is introduced into a rectangular flow chamber. Sheath fluid draws individual sample streams through the cuvette. The capillaries are closely and evenly spaced and held by a transparent retainer in a fixed position in relation to an optical detection system. Collimated sample excitation radiation is applied simultaneously across the ends of the capillaries in the retainer. Light emitted from the excited sample is detected by the optical detection system. The retainer is provided by a transparent chamber having inward slanting end walls. The capillaries are wedged into the chamber. One sideways dimension of the chamber is equal to the diameter of the capillaries and one end to end dimension varies from, at the top of the chamber, slightly greater than the sum of the diameters of the capillaries to, at the bottom of the chamber, slightly smaller than the sum of the diameters of the capillaries. The optical system utilizes optic fibres to deliver light tomore » individual photodetectors, one for each capillary tube. A filter or wavelength division demultiplexer may be used for isolating fluorescence at particular bands.« less
Inventors:
 [1];  [1]
  1. (Edmonton, CA)
Issue Date:
OSTI Identifier:
870013
Assignee:
Governors of University of Alberta (Edmonton, CA) CHO
Patent Number(s):
US 5439578
Contract Number:
FG02-91ER61123
Research Org:
University of Alberta
Country of Publication:
United States
Language:
English
Subject:
multiple; capillary; biochemical; analyzer; allows; detection; light; capillaries; reduced; interfaces; pass; detecting; emitted; sample; analyzed; modified; sheath; flow; cuvette; linear; rectangular; array; introduced; chamber; fluid; draws; individual; streams; closely; spaced; held; transparent; retainer; fixed; position; relation; optical; collimated; excitation; radiation; applied; simultaneously; excited; detected; provided; inward; slanting; walls; wedged; sideways; dimension; equal; diameter; varies; top; slightly; diameters; bottom; utilizes; optic; fibres; deliver; photodetectors; tube; filter; wavelength; division; demultiplexer; isolating; fluorescence; particular; bands; sample stream; optical detection; excitation radiation; sheath flow; multiple capillaries; capillary tube; light emitted; fixed position; flow chamber; sheath fluid; rectangular array; wavelength division; capillary biochemical; biochemical analyzer; sample streams; multiple capillary; detecting light; chemical analyzer; individual sample; rectangular flow; allows detection; /204/356/