Multiple capillary biochemical analyzer with barrier member

Dovichi, N J; Zhang, J Z

Title: Multiple capillary biochemical analyzer with barrier member

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Abstract

A multiple capillary biochemical analyzer is disclosed for sequencing DNA and performing other analyses, in which a set of capillaries extends from wells in a microtiter plate into a cuvette. In the cuvette the capillaries are held on fixed closely spaced centers by passing through a sandwich construction having a pair of metal shims which squeeze between them a rubber gasket, forming a leak proof seal for an interior chamber in which the capillary ends are positioned. Sheath fluid enters the chamber and entrains filament sample streams from the capillaries. The filament sample streams, and sheath fluid, flow through aligned holes in a barrier member spaced close to the capillary ends, into a collection chamber having a lower glass window. The filament streams are illuminated above the barrier member by a laser, causing them to fluoresce. The fluorescence is viewed end-on by a CCD camera chip located below the glass window. The arrangement ensures an equal optical path length from all fluorescing spots to the CCD chip and also blocks scattered fluorescence illumination, providing more uniform results and an improved signal-to-noise ratio. 12 figs.

Inventors:: Dovichi, N J; Zhang, J Z

Issue Date:: 1996-10-22

Research Org.:: University of Alberta

OSTI Identifier:: 392644

Patent Number(s):: 5567294

Application Number:: PAN: 8-593,877

Assignee:: Univ. of Alberta, Edmonton, AB (Canada)

DOE Contract Number:: FG02-91ER61123

Resource Type:: Patent

Resource Relation:: Other Information: PBD: 22 Oct 1996

Country of Publication:: United States

Language:: English

Subject:: 55 BIOLOGY AND MEDICINE, BASIC STUDIES; DNA SEQUENCERS; DESIGN; DNA SEQUENCING; BIOCHEMISTRY; CHEMICAL ANALYSIS

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                    Dovichi, N J, and Zhang, J Z. Multiple capillary biochemical analyzer with barrier member.  United States: N. p., 1996. 
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                    Dovichi, N J, & Zhang, J Z. Multiple capillary biochemical analyzer with barrier member.  United States.

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                    Dovichi, N J, and Zhang, J Z. Tue .  
        "Multiple capillary biochemical analyzer with barrier member".  United States.

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@article{osti_392644,

  title        = {Multiple capillary biochemical analyzer with barrier member},

  author       = {Dovichi, N J and Zhang, J Z},

  abstractNote = {A multiple capillary biochemical analyzer is disclosed for sequencing DNA and performing other analyses, in which a set of capillaries extends from wells in a microtiter plate into a cuvette. In the cuvette the capillaries are held on fixed closely spaced centers by passing through a sandwich construction having a pair of metal shims which squeeze between them a rubber gasket, forming a leak proof seal for an interior chamber in which the capillary ends are positioned. Sheath fluid enters the chamber and entrains filament sample streams from the capillaries. The filament sample streams, and sheath fluid, flow through aligned holes in a barrier member spaced close to the capillary ends, into a collection chamber having a lower glass window. The filament streams are illuminated above the barrier member by a laser, causing them to fluoresce. The fluorescence is viewed end-on by a CCD camera chip located below the glass window. The arrangement ensures an equal optical path length from all fluorescing spots to the CCD chip and also blocks scattered fluorescence illumination, providing more uniform results and an improved signal-to-noise ratio. 12 figs.},

  doi          = {},

  journal      = {},
number       = ,

  volume       = ,

  place        = {United States},

  year         = {1996},

  month        = {10}

}

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