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Title: Multiple capillary biochemical analyzer with barrier member

Abstract

A multiple capillary biochemical analyzer for sequencing DNA and performing other analyses, in which a set of capillaries extends from wells in a microtiter plate into a cuvette. In the cuvette the capillaries are held on fixed closely spaced centers by passing through a sandwich construction having a pair of metal shims which squeeze between them a rubber gasket, forming a leak proof seal for an interior chamber in which the capillary ends are positioned. Sheath fluid enters the chamber and entrains filament sample streams from the capillaries. The filament sample streams, and sheath fluid, flow through aligned holes in a barrier member spaced close to the capillary ends, into a collection chamber having a lower glass window. The filament streams are illuminated above the barrier member by a laser, causing them to fluoresce. The fluorescence is viewed end-on by a CCD camera chip located below the glass window. The arrangement ensures an equal optical path length from all fluorescing spots to the CCD chip and also blocks scattered fluorescence illumination, providing more uniform results and an improved signal to noise ratio.

Inventors:
 [1];  [1]
  1. Edmonton, CA
Issue Date:
Research Org.:
University of Alberta
OSTI Identifier:
870647
Patent Number(s):
5567294
Assignee:
Board of Governors, University of Alberta (Alberta, CA)
Patent Classifications (CPCs):
G - PHYSICS G01 - MEASURING G01N - INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
DOE Contract Number:  
FG02-91ER61123
Resource Type:
Patent
Country of Publication:
United States
Language:
English
Subject:
multiple; capillary; biochemical; analyzer; barrier; sequencing; dna; performing; analyses; set; capillaries; extends; microtiter; plate; cuvette; held; fixed; closely; spaced; centers; passing; sandwich; construction; pair; metal; shims; squeeze; rubber; gasket; forming; leak; proof; seal; interior; chamber; positioned; sheath; fluid; enters; entrains; filament; sample; streams; flow; aligned; holes; close; collection; glass; window; illuminated; laser; causing; fluoresce; fluorescence; viewed; end-on; ccd; camera; chip; located; below; arrangement; ensures; equal; optical; path; length; fluorescing; spots; blocks; scattered; illumination; providing; uniform; results; improved; signal; noise; ratio; sample stream; located below; sequencing dna; fluid enters; glass window; ccd camera; path length; optical path; closely spaced; collection chamber; interior chamber; sheath fluid; noise ratio; capillary biochemical; biochemical analyzer; spaced close; sample streams; multiple capillary; chemical analyzer; microtiter plate; titer plate; leak proof; /204/

Citation Formats

Dovichi, Norman J, and Zhang, Jian Z. Multiple capillary biochemical analyzer with barrier member. United States: N. p., 1996. Web.
Dovichi, Norman J, & Zhang, Jian Z. Multiple capillary biochemical analyzer with barrier member. United States.
Dovichi, Norman J, and Zhang, Jian Z. Mon . "Multiple capillary biochemical analyzer with barrier member". United States. https://www.osti.gov/servlets/purl/870647.
@article{osti_870647,
title = {Multiple capillary biochemical analyzer with barrier member},
author = {Dovichi, Norman J and Zhang, Jian Z},
abstractNote = {A multiple capillary biochemical analyzer for sequencing DNA and performing other analyses, in which a set of capillaries extends from wells in a microtiter plate into a cuvette. In the cuvette the capillaries are held on fixed closely spaced centers by passing through a sandwich construction having a pair of metal shims which squeeze between them a rubber gasket, forming a leak proof seal for an interior chamber in which the capillary ends are positioned. Sheath fluid enters the chamber and entrains filament sample streams from the capillaries. The filament sample streams, and sheath fluid, flow through aligned holes in a barrier member spaced close to the capillary ends, into a collection chamber having a lower glass window. The filament streams are illuminated above the barrier member by a laser, causing them to fluoresce. The fluorescence is viewed end-on by a CCD camera chip located below the glass window. The arrangement ensures an equal optical path length from all fluorescing spots to the CCD chip and also blocks scattered fluorescence illumination, providing more uniform results and an improved signal to noise ratio.},
doi = {},
journal = {},
number = ,
volume = ,
place = {United States},
year = {1996},
month = {1}
}

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