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Title: Multiple capillary biochemical analyzer with barrier member

A multiple capillary biochemical analyzer for sequencing DNA and performing other analyses, in which a set of capillaries extends from wells in a microtiter plate into a cuvette. In the cuvette the capillaries are held on fixed closely spaced centers by passing through a sandwich construction having a pair of metal shims which squeeze between them a rubber gasket, forming a leak proof seal for an interior chamber in which the capillary ends are positioned. Sheath fluid enters the chamber and entrains filament sample streams from the capillaries. The filament sample streams, and sheath fluid, flow through aligned holes in a barrier member spaced close to the capillary ends, into a collection chamber having a lower glass window. The filament streams are illuminated above the barrier member by a laser, causing them to fluoresce. The fluorescence is viewed end-on by a CCD camera chip located below the glass window. The arrangement ensures an equal optical path length from all fluorescing spots to the CCD chip and also blocks scattered fluorescence illumination, providing more uniform results and an improved signal to noise ratio.
Inventors:
 [1];  [1]
  1. (Edmonton, CA)
Issue Date:
OSTI Identifier:
870647
Assignee:
Board of Governors, University of Alberta (Alberta, CA) CHO
Patent Number(s):
US 5567294
Contract Number:
FG02-91ER61123
Research Org:
University of Alberta
Country of Publication:
United States
Language:
English
Subject:
multiple; capillary; biochemical; analyzer; barrier; sequencing; dna; performing; analyses; set; capillaries; extends; microtiter; plate; cuvette; held; fixed; closely; spaced; centers; passing; sandwich; construction; pair; metal; shims; squeeze; rubber; gasket; forming; leak; proof; seal; interior; chamber; positioned; sheath; fluid; enters; entrains; filament; sample; streams; flow; aligned; holes; close; collection; glass; window; illuminated; laser; causing; fluoresce; fluorescence; viewed; end-on; ccd; camera; chip; located; below; arrangement; ensures; equal; optical; path; length; fluorescing; spots; blocks; scattered; illumination; providing; uniform; results; improved; signal; noise; ratio; sample stream; located below; sequencing dna; fluid enters; glass window; ccd camera; path length; optical path; closely spaced; collection chamber; interior chamber; sheath fluid; noise ratio; capillary biochemical; biochemical analyzer; spaced close; sample streams; multiple capillary; chemical analyzer; microtiter plate; titer plate; leak proof; /204/