Data for "Examining Organic Acid Production Potential and Growth-Coupled Strategies in Issatchenkia orientalis Using Constraint-Based Modeling"

Suthers, Patrick; Maranas, Costas

doi:10.13012/B2IDB-2926789_V1

Title: Data for "Examining Organic Acid Production Potential and Growth-Coupled Strategies in Issatchenkia orientalis Using Constraint-Based Modeling"

Dataset
Other Related Research

Abstract

Growth-coupling product formation can facilitate strain stability by aligning industrial objectives with biological fitness. Organic acids make up many building block chemicals that can be produced from sugars obtainable from renewable biomass. Issatchenkia orientalis is a yeast strain tolerant to acidic conditions and is thus a promising host for industrial production of organic acids. Here, we use constraint-based methods to assess the potential of computationally designing growth-coupled production strains for I. orientalis that produce 22 different organic acids under aerobic or microaerobic conditions. We explore native and engineered pathways using glucose or xylose as the carbon substrates as proxy constituents of hydrolyzed biomass. We identified growth-coupled production strategies for 37 of the substrate-product pairs, with 15 pairs achieving production for any growth rate. We systematically assess the strain design solutions and categorize the underlying principles involved.

Authors:

;

Department of Chemical Engineering, The Pennsylvania State University, University Park, Pennsylvania, USA; Center for Advanced Bioenergy and Bioproducts Innovation, The Pennsylvania State University, University Park, Pennsylvania, USA

Publication Date:: Sun May 22 20:00:00 EDT 2022

DOE Contract Number:: SC0018420

Research Org.:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of Illinois Urbana-Champaign

Sponsoring Org.:: U.S. Department of Energy (DOE)

Subject:: Bioproducts; Modeling

OSTI Identifier:: 3015972

DOI:: https://doi.org/10.13012/B2IDB-2926789_V1

Citation Formats


                    Suthers, Patrick, and Maranas, Costas. Data for "Examining Organic Acid Production Potential and Growth-Coupled Strategies in Issatchenkia orientalis Using Constraint-Based Modeling".  United States: N. p., 2022. 
        Web.  doi:10.13012/B2IDB-2926789_V1.

Copy to clipboard


                    Suthers, Patrick, & Maranas, Costas. Data for "Examining Organic Acid Production Potential and Growth-Coupled Strategies in Issatchenkia orientalis Using Constraint-Based Modeling".  United States.  doi:https://doi.org/10.13012/B2IDB-2926789_V1

Copy to clipboard


                    Suthers, Patrick, and Maranas, Costas. 2022.  
"Data for "Examining Organic Acid Production Potential and Growth-Coupled Strategies in Issatchenkia orientalis Using Constraint-Based Modeling"".  United States.  doi:https://doi.org/10.13012/B2IDB-2926789_V1.  https://www.osti.gov/servlets/purl/3015972. Pub date:Sun May 22 20:00:00 EDT 2022

Copy to clipboard


                    
@article{osti_3015972,

  title        = {Data for "Examining Organic Acid Production Potential and Growth-Coupled Strategies in Issatchenkia orientalis Using Constraint-Based Modeling"},

  author       = {Suthers, Patrick and Maranas, Costas},

  abstractNote = {Growth-coupling product formation can facilitate strain stability by aligning industrial objectives with biological fitness. Organic acids make up many building block chemicals that can be produced from sugars obtainable from renewable biomass. Issatchenkia orientalis is a yeast strain tolerant to acidic conditions and is thus a promising host for industrial production of organic acids. Here, we use constraint-based methods to assess the potential of computationally designing growth-coupled production strains for I. orientalis that produce 22 different organic acids under aerobic or microaerobic conditions. We explore native and engineered pathways using glucose or xylose as the carbon substrates as proxy constituents of hydrolyzed biomass. We identified growth-coupled production strategies for 37 of the substrate-product pairs, with 15 pairs achieving production for any growth rate. We systematically assess the strain design solutions and categorize the underlying principles involved.},

  doi          = {10.13012/B2IDB-2926789_V1},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Sun May 22 20:00:00 EDT 2022},

  month        = {Sun May 22 20:00:00 EDT 2022}

}

Copy to clipboard

Dataset:

View Dataset

DOI: https://doi.org/10.13012/B2IDB-2926789_V1

Save / Share:

Export Metadata

Save to My Library

Similar records in DOE Data Explorer and OSTI.GOV collections:

Examining organic acid production potential and growth–coupled strategies in Issatchenkia orientalis using constraint–based modeling

Journal Article Suthers, Patrick F. ; Maranas, Costas D. - Biotechnology Progress

Growth-coupling product formation can facilitate strain stability by aligning industrial objectives with biological fitness. Organic acids make up many building block chemicals that can be produced from sugars obtainable from renewable biomass. Issatchenkia orientalis is a yeast strain tolerant to acidic conditions and is thus a promising host for industrial production of organic acids. Here, we use constraint-based methods to assess the potential of computationally designing growth-coupled production strains for I. orientalis that produce 22 different organic acids under aerobic or microaerobic conditions. We explore native and engineered pathways using glucose or xylose as the carbon substrates as proxy constituentsmore »« less
Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling

Journal Article Suthers, Patrick F. ; Maranas, Costas D. - Biotechnology Progress

Abstract Growth‐coupling product formation can facilitate strain stability by aligning industrial objectives with biological fitness. Organic acids make up many building block chemicals that can be produced from sugars obtainable from renewable biomass. Issatchenkia orientalis is a yeast strain tolerant to acidic conditions and is thus a promising host for industrial production of organic acids. Here, we use constraint‐based methods to assess the potential of computationally designing growth‐coupled production strains for I. orientalis that produce 22 different organic acids under aerobic or microaerobic conditions. We explore native and engineered pathways using glucose or xylose as the carbon substrates as proxymore »« less
Examining organic acid production potential and growth‐coupled strategies in Issatchenkia orientalis using constraint‐based modeling

Journal Article Suthers, Patrick F. ; Maranas, Costas D. - Biotechnology Progress

Abstract Growth‐coupling product formation can facilitate strain stability by aligning industrial objectives with biological fitness. Organic acids make up many building block chemicals that can be produced from sugars obtainable from renewable biomass. Issatchenkia orientalis is a yeast strain tolerant to acidic conditions and is thus a promising host for industrial production of organic acids. Here, we use constraint‐based methods to assess the potential of computationally designing growth‐coupled production strains for I. orientalis that produce 22 different organic acids under aerobic or microaerobic conditions. We explore native and engineered pathways using glucose or xylose as the carbon substrates as proxymore »« less
Data for Self-Buffering System for Cost-Effective Production of Lactic Acid from Glucose and Xylose Using Acid-Tolerant Issatchenkia orientalis

Dataset Lee, Ye-Gi ; Kang, Nam Kyu ; Kim, Chanwoo ; ...

This study presents a cost-effective strategy for producing organic acids from glucose and xylose using the acid-tolerant yeast, Issatchenkia orientalis. I. orientalis was engineered to produce lactic acid from xylose, and the resulting strain, SD108XL, successfully converted sorghum hydrolysates into lactic acid. In order to enable low-pH fermentation, a self-buffering strategy, where the lactic acid generated by the SD108XL strain during fermentation served as a buffer, was developed. As a result, the SD108 strain produced 67 g/L of lactic acid from 73 g/L of glucose and 40 g/L of xylose, simulating a sugar composition of sorghum biomass hydrolysates. Moreover, techno-economicmore »« less
Data for "Decompartmentalization of the yeast mitochondrial metabolism to improve chemical production in Issatchenkia orientalis"

Dataset Zhao, Huimin ; Rabinowitz, Joshua ; Guest, Jeremy ; ...

Microbial production of chemicals may suffer from inadequate cofactor provision, a challenge further exacerbated in yeasts due to compartmentalized cofactor metabolism. Here, we perform cofactor engineering through the decompartmentalization of mitochondrial metabolism to improve succinic acid (SA) production in Issatchenkia orientalis. We localize the reducing equivalents of mitochondrial NADH to the cytosol through cytosolic expression of its pyruvate dehydrogenase (PDH) complex and couple a reductive tricarboxylic acid pathway with a glyoxylate shunt, partially bypassing an NADH-dependent malate dehydrogenase to conserve NADH. Cytosolic SA production reaches a titer of 104 g/L and a yield of 0.85 g/g glucose, surpassing the yield of 0.66 g/gmore »« less

Similar Records