Data for Self-Buffering System for Cost-Effective Production of Lactic Acid from Glucose and Xylose Using Acid-Tolerant Issatchenkia orientalis

Lee, Ye-Gi; Kang, Nam Kyu; Kim, Chanwoo; Tran, Vinh; Cao, Mingfeng; Yoshikuni, Yasuo; Zhao, Huimin; Jin, Yong-Su

doi:10.13012/B2IDB-4728338_V1

Title: Data for Self-Buffering System for Cost-Effective Production of Lactic Acid from Glucose and Xylose Using Acid-Tolerant Issatchenkia orientalis

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Abstract

This study presents a cost-effective strategy for producing organic acids from glucose and xylose using the acid-tolerant yeast, Issatchenkia orientalis. I. orientalis was engineered to produce lactic acid from xylose, and the resulting strain, SD108XL, successfully converted sorghum hydrolysates into lactic acid. In order to enable low-pH fermentation, a self-buffering strategy, where the lactic acid generated by the SD108XL strain during fermentation served as a buffer, was developed. As a result, the SD108 strain produced 67 g/L of lactic acid from 73 g/L of glucose and 40 g/L of xylose, simulating a sugar composition of sorghum biomass hydrolysates. Moreover, techno-economic analysis underscored the efficiency of the self-buffering strategy in streamlining the downstream process, thereby reducing production costs. These results demonstrate the potential of I. orientalis as a platform strain for the cost-effective production of organic acids from cellulosic hydrolysates.

Authors:

Lee, Ye-Gi;

; Kim, Chanwoo;

;

; Zhao, Huimin; Jin, Yong-Su

Department of Bio and Fermentation Convergence Technology and Center for Bioconvergence, Kookmin University, Seoul 02707, Korea; Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Department of Chemical Engineering, College of Engineering, Kyung Hee University, Yongin, 17104, Republic of Korea; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
arl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Department of Chemical and Biomolecular Engineering, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
US Department of Energy Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Publication Date:: Wed Mar 27 00:00:00 UTC 2024

DOE Contract Number:: SC0018420

Research Org.:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); University of Illinois Urbana-Champaign

Sponsoring Org.:: U.S. Department of Energy (DOE)

Subject:: Conversion; Gene Editing; Hydrolysate; Metabolic Engineering

OSTI Identifier:: 3014796

DOI:: https://doi.org/10.13012/B2IDB-4728338_V1

Citation Formats


                    Lee, Ye-Gi, Kang, Nam Kyu, Kim, Chanwoo, Tran, Vinh, Cao, Mingfeng, Yoshikuni, Yasuo, Zhao, Huimin, and Jin, Yong-Su. Data for Self-Buffering System for Cost-Effective Production of Lactic Acid from Glucose and Xylose Using Acid-Tolerant Issatchenkia orientalis.  United States: N. p., 2024. 
        Web.  doi:10.13012/B2IDB-4728338_V1.

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                    Lee, Ye-Gi, Kang, Nam Kyu, Kim, Chanwoo, Tran, Vinh, Cao, Mingfeng, Yoshikuni, Yasuo, Zhao, Huimin, & Jin, Yong-Su. Data for Self-Buffering System for Cost-Effective Production of Lactic Acid from Glucose and Xylose Using Acid-Tolerant Issatchenkia orientalis.  United States.  doi:https://doi.org/10.13012/B2IDB-4728338_V1

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                    Lee, Ye-Gi, Kang, Nam Kyu, Kim, Chanwoo, Tran, Vinh, Cao, Mingfeng, Yoshikuni, Yasuo, Zhao, Huimin, and Jin, Yong-Su. 2024.  
"Data for Self-Buffering System for Cost-Effective Production of Lactic Acid from Glucose and Xylose Using Acid-Tolerant Issatchenkia orientalis".  United States.  doi:https://doi.org/10.13012/B2IDB-4728338_V1.  https://www.osti.gov/servlets/purl/3014796. Pub date:Wed Mar 27 00:00:00 UTC 2024

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@article{osti_3014796,

  title        = {Data for Self-Buffering System for Cost-Effective Production of Lactic Acid from Glucose and Xylose Using Acid-Tolerant Issatchenkia orientalis},

  author       = {Lee, Ye-Gi and Kang, Nam Kyu and Kim, Chanwoo and Tran, Vinh and Cao, Mingfeng and Yoshikuni, Yasuo and Zhao, Huimin and Jin, Yong-Su},

  abstractNote = {This study presents a cost-effective strategy for producing organic acids from glucose and xylose using the acid-tolerant yeast, Issatchenkia orientalis. I. orientalis was engineered to produce lactic acid from xylose, and the resulting strain, SD108XL, successfully converted sorghum hydrolysates into lactic acid. In order to enable low-pH fermentation, a self-buffering strategy, where the lactic acid generated by the SD108XL strain during fermentation served as a buffer, was developed. As a result, the SD108 strain produced 67 g/L of lactic acid from 73 g/L of glucose and 40 g/L of xylose, simulating a sugar composition of sorghum biomass hydrolysates. Moreover, techno-economic analysis underscored the efficiency of the self-buffering strategy in streamlining the downstream process, thereby reducing production costs. These results demonstrate the potential of I. orientalis as a platform strain for the cost-effective production of organic acids from cellulosic hydrolysates.},

  doi          = {10.13012/B2IDB-4728338_V1},

  journal      = {},

  number       = ,

  volume       = ,

  place        = {United States},

  year         = {Wed Mar 27 00:00:00 UTC 2024},

  month        = {Wed Mar 27 00:00:00 UTC 2024}

}

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DOI: https://doi.org/10.13012/B2IDB-4728338_V1

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Self-Buffering system for Cost-Effective production of lactic acid from glucose and xylose using Acid-Tolerant Issatchenkia orientalis

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