Self-Buffering system for Cost-Effective production of lactic acid from glucose and xylose using Acid-Tolerant Issatchenkia orientalis

Lee, Ye-Gi; Kang, Nam Kyu; Kim, Chanwoo; Tran, Vinh G.; Cao, Mingfeng; Yoshikuni, Yasuo; Zhao, Huimin; Jin, Yong-Su

doi:10.1016/j.biortech.2024.130641

Self-Buffering system for Cost-Effective production of lactic acid from glucose and xylose using Acid-Tolerant Issatchenkia orientalis

Journal Article · Wed Mar 27 00:00:00 EDT 2024 · Bioresource Technology

DOI:https://doi.org/10.1016/j.biortech.2024.130641· OSTI ID:2377418

^[1]; Kang, Nam Kyu ^[2]; ^[3]; Tran, Vinh G. ^[3]; ^[3]; Yoshikuni, Yasuo ^[4]; ^[3]; ^[3]

Kookmin University, Seoul (Korea); University of Illinois at Urbana-Champaign, IL (United States); University of Illinois Urbana-Champaign
Kyung Hee University, Yongin (Korea, Republic of)
University of Illinois at Urbana-Champaign, IL (United States)
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

This study presents a cost-effective strategy for producing organic acids from glucose and xylose using the acid- tolerant yeast, Issatchenkia orientalis. I. orientalis was engineered to produce lactic acid from xylose, and the resulting strain, SD108XL, successfully converted sorghum hydrolysates into lactic acid. In order to enable low- pH fermentation, a self-buffering strategy, where the lactic acid generated by the SD108XL strain during fermentation served as a buffer, was developed. As a result, the SD108 strain produced 67 g/L of lactic acid from 73 g/L of glucose and 40 g/L of xylose, simulating a sugar composition of sorghum biomass hydrolysates. Moreover, techno-economic analysis underscored the efficiency of the self-buffering strategy in streamlining the downstream process, thereby reducing production costs. These results demonstrate the potential of I. orientalis as a platform strain for the cost-effective production of organic acids from cellulosic hydrolysates.

View Accepted Manuscript (DOE)

Research Organization:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC02-05CH11231; SC0018420

OSTI ID:: 2377418

Journal Information:: Bioresource Technology, Journal Name: Bioresource Technology Vol. 399; ISSN 0960-8524

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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